ATP bioluminescence: Surface hygiene monitoring in milk preparation room of neonatal intensive care unit

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

ATP Bioluminescence application and standard microbiological analyses were used to evaluate the cleanliness of milk contact surfaces and non-milk contact surfaces in milk preparation room of neonatal intensive care unit (NICU) of Universiti Kebangsaan Malaysia Medical Centre (UKMMC). A total of 44 samples including the breast pump, milk bottle, milk bottle screw top and screw ring, teats, measuring cups, waterless warmer, refrigerator, dishwasher and pasteurizer inner wall were tested on May 2017. 3M Clean and Trace Hygiene Monitoring (UXL100 ATP Test swabs) and the bioluminescence reader Clean-Trace NG Luminometer (3M) were used to measure the Relative Light Unit (RLU) and microbiological analysis using 3M Quick Swab and 3MTM PetrifilmTM for enumeration of aerobic count, Staphylococcus aureus, Enterobacteriaceae, coliform and detection of Escherichia coli (CFU /100cm2 or utensil/item). The RLU values were from 11 to 194 and passed the ATP benchmark for intensive care unit (ICU), < 250 RLU as recommended. Aerobic colony count was only found in waterless warmer (0.05±0.01 mean log CFU/warmer). None of S. aureus, Enterobacteriaceae, E. coli and coliform was detected in all samples. A weak correlation was found between bioluminescence measurements RLU and the microbiological analysis (CFU). However, the use of ATP bioluminescence in monitoring milk preparation room cleanliness can be a useful method for assessing rapidly the surface hygiene as well as to verify the Sanitation Standard Operating Procedure (SSOP) prior to implementation of Hazard Analysis and Critical Control Points (HACCP) in milk preparation room.

Original languageEnglish
Title of host publication2017 UKM FST Postgraduate Colloquium
Subtitle of host publicationProceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium
PublisherAmerican Institute of Physics Inc.
Volume1940
ISBN (Electronic)9780735416321
DOIs
Publication statusPublished - 4 Apr 2018
Event2017 UKM FST Postgraduate Colloquium - Selangor, Malaysia
Duration: 12 Jul 201713 Jul 2017

Other

Other2017 UKM FST Postgraduate Colloquium
CountryMalaysia
CitySelangor
Period12/7/1713/7/17

Fingerprint

hygiene
bioluminescence
milk
adenosine triphosphate
rooms
preparation
cleanliness
bottles
screws
sanitation
Malaysia
enumeration
staphylococcus
refrigerators
readers
Escherichia
breast
hazards
pumps
rings

Keywords

  • ATP Bioluminescence
  • milk preparation room
  • neonatal intensive care unit
  • surface hygiene monitoring

ASJC Scopus subject areas

  • Physics and Astronomy(all)

Cite this

Mohamad, M., Ishak, S., Jaafar, R., & Abdullah Sani, N. (2018). ATP bioluminescence: Surface hygiene monitoring in milk preparation room of neonatal intensive care unit. In 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium (Vol. 1940). [020091] American Institute of Physics Inc.. https://doi.org/10.1063/1.5028006

ATP bioluminescence : Surface hygiene monitoring in milk preparation room of neonatal intensive care unit. / Mohamad, Mahirah; Ishak, Shareena; Jaafar, Rohana; Abdullah Sani, Norrakiah.

2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940 American Institute of Physics Inc., 2018. 020091.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Mohamad, M, Ishak, S, Jaafar, R & Abdullah Sani, N 2018, ATP bioluminescence: Surface hygiene monitoring in milk preparation room of neonatal intensive care unit. in 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. vol. 1940, 020091, American Institute of Physics Inc., 2017 UKM FST Postgraduate Colloquium, Selangor, Malaysia, 12/7/17. https://doi.org/10.1063/1.5028006
Mohamad M, Ishak S, Jaafar R, Abdullah Sani N. ATP bioluminescence: Surface hygiene monitoring in milk preparation room of neonatal intensive care unit. In 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940. American Institute of Physics Inc. 2018. 020091 https://doi.org/10.1063/1.5028006
Mohamad, Mahirah ; Ishak, Shareena ; Jaafar, Rohana ; Abdullah Sani, Norrakiah. / ATP bioluminescence : Surface hygiene monitoring in milk preparation room of neonatal intensive care unit. 2017 UKM FST Postgraduate Colloquium: Proceedings of the University Kebangsaan Malaysia, Faculty of Science and Technology 2017 Postgraduate Colloquium. Vol. 1940 American Institute of Physics Inc., 2018.
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