Ataxia-telangiectasia cell extracts confer radioresistant DNA synthesis on control cells

Rahmah Mohamed, M. F. Lavin

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

We have investigated in greater detail the radioresistant DNA synthesis universally observed in cells from patients with ataxia-telangiectasia (A-T). The approach employed in this study was to permeabilize cells with lysolecithin after γ-irradiation and thus facilitate the introduction of cell extract into these cells. This permeabilization can be reversed by diluting the cells in growth medium. Cells treated in this way show the characteristic inhibition (control cells) or lack of it (A-T cells) after exposure to ionizing radiation. Introduction of A-T cells extracts into control cells prevented the radiationinduced inhibition of DNA synthesis normally observed in these cells. A-T cell extracts did not change the level of radioresistant DNA synthesis in A-T cells. Control cell extracts on the other hand did not influence the pattern of inhibition of DNA synthesis in either cell type. It seems likely that the agent involved is a protein because of its heat lability and sensitivity to trypsin digestion. It has a molecular weight (MW) in the range 20-30000 D. The development of this assay system for a factor conferring radioresistant DNA synthesis on control cells provides a means of purifying this factor, and ultimately an approach to identifying the gene responsible.

Original languageEnglish
Pages (from-to)337-348
Number of pages12
JournalExperimental Cell Research
Volume163
Issue number2
DOIs
Publication statusPublished - 1986
Externally publishedYes

Fingerprint

Ataxia Telangiectasia
Cell Extracts
DNA
T-Lymphocytes
Lysophosphatidylcholines
Ionizing Radiation
Trypsin
Digestion
Hot Temperature
Molecular Weight

ASJC Scopus subject areas

  • Cell Biology

Cite this

Ataxia-telangiectasia cell extracts confer radioresistant DNA synthesis on control cells. / Mohamed, Rahmah; Lavin, M. F.

In: Experimental Cell Research, Vol. 163, No. 2, 1986, p. 337-348.

Research output: Contribution to journalArticle

Mohamed, Rahmah ; Lavin, M. F. / Ataxia-telangiectasia cell extracts confer radioresistant DNA synthesis on control cells. In: Experimental Cell Research. 1986 ; Vol. 163, No. 2. pp. 337-348.
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