Associations between primary hypertension and genes in the renin angiotensin system: A prospective two-center study in university kebangsaan malaysia medical center and international medical university cardiology clinic

Norsham Juliana, Shaiful Yahaya, Abdul Latiff Mohamed, Roslan Harun

    Research output: Contribution to journalArticle

    Abstract

    This study targeted two candidate genes from the best known regulator of blood pressure; the rennin angiotensin system; the ACE gene lID polymorphism and the angiotensinogen M235T polymorphism. The study aimed to determine the genotypes trend between two different populations; the primary hypertensive patients, and the normal populations. 126 subjects were involved in this study (86 primary hypertensive patients and 40 normal individuals). All demographic factors were considered and analyzed. Insertion1deletion polymorphisms of the ACE gene were determined by an assay based on the polymerase chain reaction (PCR). Polymorphism analysis using PCR-RFLP procedure was used to identify the missense mutation M235T of the AGT gene. All significant data was collected using standardized case report form. The association of the different genotypes and the subjects' condition was analyzed using the chi squared and odds ratio analyses. In the pooled analysis of both groups, it was shown that the polymorphisms in these genes were significantly associated with the incidence of primary hypertension, p>O.O5. Results also showed that the D allele of the ACE gene may be associated with increased risk of primary hypertension (p>O.O5, OR: 3.0 [CI: 1.25 - 5.35]). The angiotensinogen M235T polymorphism also showed a significant result; the T allele is associated with increased risk of primary hypertension (p>O.O5, OR: 2.56[C.I: 1.55 -5.28]). This knowledge of the candidate genes of rennin angiotensin system has rendered it possible to show that gene polymorphism in symphony leads to the individual risk of primary hypertension.

    Original languageEnglish
    Pages (from-to)19-24
    Number of pages6
    JournalMalaysian Journal of Medicine and Health Sciences
    Volume6
    Issue number2
    Publication statusPublished - Jun 2010

    Fingerprint

    Malaysia
    Renin-Angiotensin System
    Cardiology
    Hypertension
    Genes
    Chymosin
    Angiotensinogen
    Angiotensins
    Alleles
    Genotype
    Polymerase Chain Reaction
    Missense Mutation
    Restriction Fragment Length Polymorphisms
    Population
    Odds Ratio
    Demography
    Blood Pressure
    Incidence

    Keywords

    • ACE
    • Hypertension
    • M235T
    • Rennin

    ASJC Scopus subject areas

    • Medicine(all)

    Cite this

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    title = "Associations between primary hypertension and genes in the renin angiotensin system: A prospective two-center study in university kebangsaan malaysia medical center and international medical university cardiology clinic",
    abstract = "This study targeted two candidate genes from the best known regulator of blood pressure; the rennin angiotensin system; the ACE gene lID polymorphism and the angiotensinogen M235T polymorphism. The study aimed to determine the genotypes trend between two different populations; the primary hypertensive patients, and the normal populations. 126 subjects were involved in this study (86 primary hypertensive patients and 40 normal individuals). All demographic factors were considered and analyzed. Insertion1deletion polymorphisms of the ACE gene were determined by an assay based on the polymerase chain reaction (PCR). Polymorphism analysis using PCR-RFLP procedure was used to identify the missense mutation M235T of the AGT gene. All significant data was collected using standardized case report form. The association of the different genotypes and the subjects' condition was analyzed using the chi squared and odds ratio analyses. In the pooled analysis of both groups, it was shown that the polymorphisms in these genes were significantly associated with the incidence of primary hypertension, p>O.O5. Results also showed that the D allele of the ACE gene may be associated with increased risk of primary hypertension (p>O.O5, OR: 3.0 [CI: 1.25 - 5.35]). The angiotensinogen M235T polymorphism also showed a significant result; the T allele is associated with increased risk of primary hypertension (p>O.O5, OR: 2.56[C.I: 1.55 -5.28]). This knowledge of the candidate genes of rennin angiotensin system has rendered it possible to show that gene polymorphism in symphony leads to the individual risk of primary hypertension.",
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    author = "Norsham Juliana and Shaiful Yahaya and Mohamed, {Abdul Latiff} and Roslan Harun",
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