Association of the tumor necrosis factor alpha gene polymorphism with susceptibility and clinical-immunological findings of systemic lupus erythematosus

M. R. Azizah, S. H. Kuak, S. S. Ainol, M. N. Rahim, Y. Normaznah, K. Norella

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Abstract

The etiology of systemic lupus erythematosus (SLE) is unknown but genetic factors seem to play a role in the disease pathogenesis. The tumor necrosis factor alpha (TNFα) gene, encoded at the TNF locus in the MHC class III region, is now known to be an important candidate gene in SLE, due to the proinflammatory activities of the TNFα. The objectives of this study were to examine the role of the TNFα polymorphism for the susceptibility of Malaysian Chinese lupus patients to SLE and to determine its association with organ involvement. The allelic frequencies of the TNFα polymorphic variant (TNF2) of seventy lupus patients were determined during follow-up at the Medical Clinic of the National University Hospital Malaysia by PCR-RFLP technique. Sixty-four females and 6 males with a mean age of 33 ± 12 years were included. Clinical data were obtained from case records. Autoantibody levels were measured by ELISA. Fifty-nine ethnically-matched blood donors were used as controls. The allelic frequency of the TNF2 variant was found to be significantly increased in the patients compared to the controls (52.8% vs 33.8%). SLE patients with the polymorphic TNF2 variant were found to be at increased risk of central nervous system involvement (p = 0.004, RR = 2.59) and to have an increased frequency of anti-La antibodies (p = 0.03). In view of these findings we suggest that TNF2 variant is playing a role in conferring susceptibility to SLE and in the disease pathogenesis.

Original languageEnglish
Pages (from-to)159-163
Number of pages5
JournalAsian Pacific Journal of Allergy and Immunology
Volume22
Issue number2-3
Publication statusPublished - Jun 2004
Externally publishedYes

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Systemic Lupus Erythematosus
Tumor Necrosis Factor-alpha
Genes
Malaysia
Blood Donors
Restriction Fragment Length Polymorphisms
Autoantibodies
Anti-Idiotypic Antibodies
Central Nervous System
Enzyme-Linked Immunosorbent Assay
Polymerase Chain Reaction

ASJC Scopus subject areas

  • Immunology and Allergy

Cite this

Association of the tumor necrosis factor alpha gene polymorphism with susceptibility and clinical-immunological findings of systemic lupus erythematosus. / Azizah, M. R.; Kuak, S. H.; Ainol, S. S.; Rahim, M. N.; Normaznah, Y.; Norella, K.

In: Asian Pacific Journal of Allergy and Immunology, Vol. 22, No. 2-3, 06.2004, p. 159-163.

Research output: Contribution to journalArticle

Azizah, M. R. ; Kuak, S. H. ; Ainol, S. S. ; Rahim, M. N. ; Normaznah, Y. ; Norella, K. / Association of the tumor necrosis factor alpha gene polymorphism with susceptibility and clinical-immunological findings of systemic lupus erythematosus. In: Asian Pacific Journal of Allergy and Immunology. 2004 ; Vol. 22, No. 2-3. pp. 159-163.
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