Association of Methylentetraydrofolate reductase (MTHFR) 677 C > T gene polymorphism and homocysteine levels in psoriasis vulgaris patients from Malaysia: A case-control study

Siaw C. Liew, Esha Das-Gupta, Shew F. Wong, Nagarajah Lee, Najeeb Safdar, Adawiyah Jamil

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background: The methylenetetrahydrofolate reductase (MTHFR) enzyme catalyzes the reduction of 5, 10-methylenetetrahydrofolate to 5-methyltetrahydrofolate and methyl donors. The methyl donors are required for the conversion of homocysteine to methionine. Mutation of MTHFR 677 C > T disrupts its thermostability therefore leads to defective enzyme activities and dysregulation of homocysteine levels. Methods. This case-control study (n = 367) was conducted to investigate the correlation of the MTHFR gene polymorphism [NM-005957] and psoriasis vulgaris amongst the Malaysian population. Overnight fasting blood samples were collected from a subgroup of consented psoriasis vulgaris patients and matched controls (n = 84) for the quantification of homocysteine, vitamin B 12and folic acid levels. Results: There was no significant increase of the MTHFR 677 C > T mutation in patients with psoriasis vulgaris compared with controls (Ξ 2 = 0.733, p = 0.392). No significant association between homocysteine levels and MTHFR gene polymorphism in cases and controls were observed (F = 0.91, df = 3, 80, p = 0.44). However, homocysteine levels in cases were negatively correlated with vitamin B 12(r = -0.173) and folic acid (r = -0.345) levels. Vitamin B 12and folic acid levels in cases were also negatively correlated (r = -0.164). Conclusions: Our results indicate that there was no significant association between the MTHFR gene polymorphism and psoriasis vulgaris in the Malaysian population. There was no significant increase of the plasma homocysteine level in the psoriasis patients compared to the controls.

Original languageEnglish
Article number1
JournalNutrition Journal
Volume11
Issue number1
DOIs
Publication statusPublished - 2012
Externally publishedYes

Fingerprint

Malaysia
Homocysteine
Psoriasis
Case-Control Studies
Oxidoreductases
Folic Acid
Genes
Vitamin B Complex
Tissue Donors
Methylenetetrahydrofolate Reductase (NADPH2)
Mutation
Enzymes
Vitamin B 12
Methionine
Population
Fasting

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Association of Methylentetraydrofolate reductase (MTHFR) 677 C > T gene polymorphism and homocysteine levels in psoriasis vulgaris patients from Malaysia : A case-control study. / Liew, Siaw C.; Das-Gupta, Esha; Wong, Shew F.; Lee, Nagarajah; Safdar, Najeeb; Jamil, Adawiyah.

In: Nutrition Journal, Vol. 11, No. 1, 1, 2012.

Research output: Contribution to journalArticle

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abstract = "Background: The methylenetetrahydrofolate reductase (MTHFR) enzyme catalyzes the reduction of 5, 10-methylenetetrahydrofolate to 5-methyltetrahydrofolate and methyl donors. The methyl donors are required for the conversion of homocysteine to methionine. Mutation of MTHFR 677 C > T disrupts its thermostability therefore leads to defective enzyme activities and dysregulation of homocysteine levels. Methods. This case-control study (n = 367) was conducted to investigate the correlation of the MTHFR gene polymorphism [NM-005957] and psoriasis vulgaris amongst the Malaysian population. Overnight fasting blood samples were collected from a subgroup of consented psoriasis vulgaris patients and matched controls (n = 84) for the quantification of homocysteine, vitamin B 12and folic acid levels. Results: There was no significant increase of the MTHFR 677 C > T mutation in patients with psoriasis vulgaris compared with controls (Ξ 2 = 0.733, p = 0.392). No significant association between homocysteine levels and MTHFR gene polymorphism in cases and controls were observed (F = 0.91, df = 3, 80, p = 0.44). However, homocysteine levels in cases were negatively correlated with vitamin B 12(r = -0.173) and folic acid (r = -0.345) levels. Vitamin B 12and folic acid levels in cases were also negatively correlated (r = -0.164). Conclusions: Our results indicate that there was no significant association between the MTHFR gene polymorphism and psoriasis vulgaris in the Malaysian population. There was no significant increase of the plasma homocysteine level in the psoriasis patients compared to the controls.",
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AB - Background: The methylenetetrahydrofolate reductase (MTHFR) enzyme catalyzes the reduction of 5, 10-methylenetetrahydrofolate to 5-methyltetrahydrofolate and methyl donors. The methyl donors are required for the conversion of homocysteine to methionine. Mutation of MTHFR 677 C > T disrupts its thermostability therefore leads to defective enzyme activities and dysregulation of homocysteine levels. Methods. This case-control study (n = 367) was conducted to investigate the correlation of the MTHFR gene polymorphism [NM-005957] and psoriasis vulgaris amongst the Malaysian population. Overnight fasting blood samples were collected from a subgroup of consented psoriasis vulgaris patients and matched controls (n = 84) for the quantification of homocysteine, vitamin B 12and folic acid levels. Results: There was no significant increase of the MTHFR 677 C > T mutation in patients with psoriasis vulgaris compared with controls (Ξ 2 = 0.733, p = 0.392). No significant association between homocysteine levels and MTHFR gene polymorphism in cases and controls were observed (F = 0.91, df = 3, 80, p = 0.44). However, homocysteine levels in cases were negatively correlated with vitamin B 12(r = -0.173) and folic acid (r = -0.345) levels. Vitamin B 12and folic acid levels in cases were also negatively correlated (r = -0.164). Conclusions: Our results indicate that there was no significant association between the MTHFR gene polymorphism and psoriasis vulgaris in the Malaysian population. There was no significant increase of the plasma homocysteine level in the psoriasis patients compared to the controls.

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