Association of malaysian helicobacter pylori virulence polymorphisms with severity of gastritis and patients' ethnicity

Alfizah Hanafiah, Mohamed Ramelah, Mohd Rizal Abdul Manaf, Abdullah S. Anwar, Mohamed R. Isa

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background and aim: Polymorphisms of Helicobacter pylori cagA and vacA genes do exist and may contribute to differences in H. pylori infection and gastroduodenal diseases among races in the Malaysian population. This study was conducted to characterize the polymorphisms in H. pylori cagA and vacA in Malaysian population. Methods: A total of 110 H. pylori isolates were genotyped by PCR and sequenced for cagA and PCR-RFLP for vacA. Results: East Asian cagA was predominantly detected (64.5%), whereas vacA s1m1 and s1m2 alleles were detected in 60.9 and 37.3% of strains, respectively. A statistical association between cagA type with patients' ethnicity (p<.0001) and age group >50years old (p=027) was identified. vacA alleles showed significant association with age group >50years old (p=017) and increased neutrophil activity in gastric mucosa (p=028 and p=016 for moderate and marked activity, respectively). Further identification of vacA polymorphism revealed that 84% of strains from Malays and Indians showed one RFLP pattern (RFLP-1), whereas more than one RFLP patterns (RFLP-2, 3, 4, 5, 6, and 8) were predominantly observed in strains from Chinese (82%) (p<.0001). Increasing severity of gastric inflammation was observed in gastric mucosa infected with strains carrying RFLP-2, 3, 4, 5, and 6 (p=037). About 86.6% of H. pylori strains with East Asian cagA were vacA RFLP-2, 3, 4, 5, 6, and 8, and 88% of Western cagA strains were vacA RFLP-1 (p<.0001). Chinese and Indians are susceptible to different virulence genotypes of H. pylori, whereas Malays showed a mixed virulence genotypes. Conclusion: Marked differences in the polymorphisms of cagA and vacA were observed among strains in Malaysian population. This provides a new insight into the pathogenicity of H. pylori in multiracial population.

Original languageEnglish
Pages (from-to)340-349
Number of pages10
JournalHelicobacter
Volume17
Issue number5
DOIs
Publication statusPublished - Oct 2012

Fingerprint

Gastritis
Helicobacter pylori
Restriction Fragment Length Polymorphisms
Virulence
Gastric Mucosa
Population
Alleles
Genotype
Polymerase Chain Reaction
Helicobacter Infections
Stomach
Neutrophils
Age Groups
Inflammation
Genes

Keywords

  • East Asian cagA
  • Helicobacter pylori
  • RFLP polymorphism
  • vacA allele
  • Western cagA

ASJC Scopus subject areas

  • Gastroenterology
  • Infectious Diseases

Cite this

Association of malaysian helicobacter pylori virulence polymorphisms with severity of gastritis and patients' ethnicity. / Hanafiah, Alfizah; Ramelah, Mohamed; Abdul Manaf, Mohd Rizal; Anwar, Abdullah S.; Isa, Mohamed R.

In: Helicobacter, Vol. 17, No. 5, 10.2012, p. 340-349.

Research output: Contribution to journalArticle

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abstract = "Background and aim: Polymorphisms of Helicobacter pylori cagA and vacA genes do exist and may contribute to differences in H. pylori infection and gastroduodenal diseases among races in the Malaysian population. This study was conducted to characterize the polymorphisms in H. pylori cagA and vacA in Malaysian population. Methods: A total of 110 H. pylori isolates were genotyped by PCR and sequenced for cagA and PCR-RFLP for vacA. Results: East Asian cagA was predominantly detected (64.5{\%}), whereas vacA s1m1 and s1m2 alleles were detected in 60.9 and 37.3{\%} of strains, respectively. A statistical association between cagA type with patients' ethnicity (p<.0001) and age group >50years old (p=027) was identified. vacA alleles showed significant association with age group >50years old (p=017) and increased neutrophil activity in gastric mucosa (p=028 and p=016 for moderate and marked activity, respectively). Further identification of vacA polymorphism revealed that 84{\%} of strains from Malays and Indians showed one RFLP pattern (RFLP-1), whereas more than one RFLP patterns (RFLP-2, 3, 4, 5, 6, and 8) were predominantly observed in strains from Chinese (82{\%}) (p<.0001). Increasing severity of gastric inflammation was observed in gastric mucosa infected with strains carrying RFLP-2, 3, 4, 5, and 6 (p=037). About 86.6{\%} of H. pylori strains with East Asian cagA were vacA RFLP-2, 3, 4, 5, 6, and 8, and 88{\%} of Western cagA strains were vacA RFLP-1 (p<.0001). Chinese and Indians are susceptible to different virulence genotypes of H. pylori, whereas Malays showed a mixed virulence genotypes. Conclusion: Marked differences in the polymorphisms of cagA and vacA were observed among strains in Malaysian population. This provides a new insight into the pathogenicity of H. pylori in multiracial population.",
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AU - Hanafiah, Alfizah

AU - Ramelah, Mohamed

AU - Abdul Manaf, Mohd Rizal

AU - Anwar, Abdullah S.

AU - Isa, Mohamed R.

PY - 2012/10

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N2 - Background and aim: Polymorphisms of Helicobacter pylori cagA and vacA genes do exist and may contribute to differences in H. pylori infection and gastroduodenal diseases among races in the Malaysian population. This study was conducted to characterize the polymorphisms in H. pylori cagA and vacA in Malaysian population. Methods: A total of 110 H. pylori isolates were genotyped by PCR and sequenced for cagA and PCR-RFLP for vacA. Results: East Asian cagA was predominantly detected (64.5%), whereas vacA s1m1 and s1m2 alleles were detected in 60.9 and 37.3% of strains, respectively. A statistical association between cagA type with patients' ethnicity (p<.0001) and age group >50years old (p=027) was identified. vacA alleles showed significant association with age group >50years old (p=017) and increased neutrophil activity in gastric mucosa (p=028 and p=016 for moderate and marked activity, respectively). Further identification of vacA polymorphism revealed that 84% of strains from Malays and Indians showed one RFLP pattern (RFLP-1), whereas more than one RFLP patterns (RFLP-2, 3, 4, 5, 6, and 8) were predominantly observed in strains from Chinese (82%) (p<.0001). Increasing severity of gastric inflammation was observed in gastric mucosa infected with strains carrying RFLP-2, 3, 4, 5, and 6 (p=037). About 86.6% of H. pylori strains with East Asian cagA were vacA RFLP-2, 3, 4, 5, 6, and 8, and 88% of Western cagA strains were vacA RFLP-1 (p<.0001). Chinese and Indians are susceptible to different virulence genotypes of H. pylori, whereas Malays showed a mixed virulence genotypes. Conclusion: Marked differences in the polymorphisms of cagA and vacA were observed among strains in Malaysian population. This provides a new insight into the pathogenicity of H. pylori in multiracial population.

AB - Background and aim: Polymorphisms of Helicobacter pylori cagA and vacA genes do exist and may contribute to differences in H. pylori infection and gastroduodenal diseases among races in the Malaysian population. This study was conducted to characterize the polymorphisms in H. pylori cagA and vacA in Malaysian population. Methods: A total of 110 H. pylori isolates were genotyped by PCR and sequenced for cagA and PCR-RFLP for vacA. Results: East Asian cagA was predominantly detected (64.5%), whereas vacA s1m1 and s1m2 alleles were detected in 60.9 and 37.3% of strains, respectively. A statistical association between cagA type with patients' ethnicity (p<.0001) and age group >50years old (p=027) was identified. vacA alleles showed significant association with age group >50years old (p=017) and increased neutrophil activity in gastric mucosa (p=028 and p=016 for moderate and marked activity, respectively). Further identification of vacA polymorphism revealed that 84% of strains from Malays and Indians showed one RFLP pattern (RFLP-1), whereas more than one RFLP patterns (RFLP-2, 3, 4, 5, 6, and 8) were predominantly observed in strains from Chinese (82%) (p<.0001). Increasing severity of gastric inflammation was observed in gastric mucosa infected with strains carrying RFLP-2, 3, 4, 5, and 6 (p=037). About 86.6% of H. pylori strains with East Asian cagA were vacA RFLP-2, 3, 4, 5, 6, and 8, and 88% of Western cagA strains were vacA RFLP-1 (p<.0001). Chinese and Indians are susceptible to different virulence genotypes of H. pylori, whereas Malays showed a mixed virulence genotypes. Conclusion: Marked differences in the polymorphisms of cagA and vacA were observed among strains in Malaysian population. This provides a new insight into the pathogenicity of H. pylori in multiracial population.

KW - East Asian cagA

KW - Helicobacter pylori

KW - RFLP polymorphism

KW - vacA allele

KW - Western cagA

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