Assessment of tumourigenic potential in long-term cryopreserved human adipose-derived stem cells

Kar Wey Yong, Wan Kamarul Zaman Wan Safwani, Feng Xu, Xiaohui Zhang, Jane Ru Choi, Wan Abu Bakar Wan Abas, Siti Zawiah Omar, Mat Adenan Noor Azmi, Chua Kien Hui, Belinda Pingguan-Murphy

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cryopreservation represents an efficient way to preserve human mesenchymal stem cells (hMSCs) at early culture/passage, and allows pooling of cells to achieve sufficient cells required for off-the-shelf use in clinical applications, e.g. cell-based therapies and regenerative medicine. To fully apply cryopreserved hMSCs in a clinical setting, it is necessary to evaluate their biosafety, e.g. chromosomal abnormality and tumourigenic potential. To date, many studies have demonstrated that cryopreserved hMSCs display no chromosomal abnormalities. However, the tumourigenic potential of cryopreserved hMSCs has not yet been evaluated. In the present study, we cryopreserved human adipose-derived mesenchymal stem cells (hASCs) for 3months, using a slow freezing method with various cryoprotective agents (CPAs), followed by assessment of the tumourigenic potential of the cryopreserved hASCs after thawing and subculture. We found that long-term cryopreserved hASCs maintained normal levels of the tumour suppressor markers p53, p21, p16 and pRb, hTERT, telomerase activity and telomere length. Further, we did not observe significant DNA damage or signs of p53 mutation in cryopreserved hASCs. Our findings suggest that long-term cryopreserved hASCs are at low risk of tumourigenesis. These findings aid in establishing the biosafety profile of cryopreserved hASCs, and thus establishing low hazardous risk perception with the use of long-term cryopreserved hASCs for future clinical applications.

Original languageEnglish
JournalJournal of Tissue Engineering and Regenerative Medicine
DOIs
Publication statusAccepted/In press - 2016

Fingerprint

Stem cells
Mesenchymal Stromal Cells
Stem Cells
Chromosome Aberrations
Cryoprotective Agents
Risk perception
Thawing
Regenerative Medicine
Telomerase
Cryopreservation
Telomere
Tumor Biomarkers
Cell- and Tissue-Based Therapy
Cell culture
Freezing
DNA Damage
Tumors
DNA
Mutation

Keywords

  • Biosafety
  • Clinical applications
  • Human adipose-derived stem cells
  • Long-term cryopreservation
  • Low risk
  • Tumourigenic potential assessment

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Biomaterials

Cite this

Yong, K. W., Safwani, W. K. Z. W., Xu, F., Zhang, X., Choi, J. R., Abas, W. A. B. W., ... Pingguan-Murphy, B. (Accepted/In press). Assessment of tumourigenic potential in long-term cryopreserved human adipose-derived stem cells. Journal of Tissue Engineering and Regenerative Medicine. https://doi.org/10.1002/term.2120

Assessment of tumourigenic potential in long-term cryopreserved human adipose-derived stem cells. / Yong, Kar Wey; Safwani, Wan Kamarul Zaman Wan; Xu, Feng; Zhang, Xiaohui; Choi, Jane Ru; Abas, Wan Abu Bakar Wan; Omar, Siti Zawiah; Azmi, Mat Adenan Noor; Kien Hui, Chua; Pingguan-Murphy, Belinda.

In: Journal of Tissue Engineering and Regenerative Medicine, 2016.

Research output: Contribution to journalArticle

Yong, Kar Wey ; Safwani, Wan Kamarul Zaman Wan ; Xu, Feng ; Zhang, Xiaohui ; Choi, Jane Ru ; Abas, Wan Abu Bakar Wan ; Omar, Siti Zawiah ; Azmi, Mat Adenan Noor ; Kien Hui, Chua ; Pingguan-Murphy, Belinda. / Assessment of tumourigenic potential in long-term cryopreserved human adipose-derived stem cells. In: Journal of Tissue Engineering and Regenerative Medicine. 2016.
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