Assessment of cytotoxicity and genotoxicity of stem bark extracts from Canarium odontophyllum Miq. (dabai) against HCT 116 human colorectal cancer cell line

Dayang Fredalina Basri, Zafira Ayushah Zainul Alamin, Chan Kok Meng

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9 Citations (Scopus)

Abstract

Background: Canarium odontophyllum Miq. is a plant species widely known as 'dabai' and can be vastly found in Sarawak. The aim of this study was to assess the cytotoxic and genotoxic effect of extracts from stem bark of C. odontophyllum against HCT 116 human colorectal cancer cell line. Method: The IC50 values of the aqueous, methanol, and acetone extracts against HCT 116 cells as well as the acetone extract against human colon fibroblast cell line CCD-18co were determined using the MTT assay. The concentration of the extracts ranged from 12.5 to 200μg/ml at treatment time of 24, 48 and 72h. Annexin V-FITC/PI labelling assay was employed to determine mode of HCT 116 cell death induced by acetone extract at 48h. The DNA damage induced by the extract in HCT 116 cells was detected using alkaline comet assay at 30min of IC10 and IC25 treatment. Results: Acetone extract exhibited the highest cytotoxic effect against HCT 116 cells compared to methanol and aqueous extract at 24, 48 and 72h. Despite no cytotoxic effect by acetone extract against CCD-18co cells at 24 and 48h, however at 72h, CCD-18co cells proliferated. Apoptosis assessment using Annexin V-FITC/PI labelling assay revealed that the primary cell death was via apoptosis after 48h treatment. Low doses of acetone extract from stem bark of C. odontophyllum showed significant DNA damage in HCT 116 cells with tail moment of 6.187 ± 0.718 A.U and 7.877 ± 0.142 A.U, respectively. Conclusions: Acetone extract from stem bark of C. odontophyllum has high potential in the development of anticancer agent against HCT 116 cells with no cytotoxic effect against human colon fibroblast cells.

Original languageEnglish
Article number36
JournalBMC Complementary and Alternative Medicine
Volume16
Issue number1
DOIs
Publication statusPublished - 29 Jan 2016

Fingerprint

HCT116 Cells
Acetone
Colorectal Neoplasms
Cell Line
Fluorescein-5-isothiocyanate
Annexin A5
DNA Damage
Methanol
Colon
Cell Death
Fibroblasts
Apoptosis
Comet Assay
Malaysia
Antineoplastic Agents
Inhibitory Concentration 50
Tail

Keywords

  • Apoptosis
  • Canarium odontophyllum
  • CCD-18co
  • Colorectal cancer
  • Cytotoxicity
  • Dabai
  • DNA damage
  • HCT 116

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

@article{fc481ea982084866b2ae48732e85ecfb,
title = "Assessment of cytotoxicity and genotoxicity of stem bark extracts from Canarium odontophyllum Miq. (dabai) against HCT 116 human colorectal cancer cell line",
abstract = "Background: Canarium odontophyllum Miq. is a plant species widely known as 'dabai' and can be vastly found in Sarawak. The aim of this study was to assess the cytotoxic and genotoxic effect of extracts from stem bark of C. odontophyllum against HCT 116 human colorectal cancer cell line. Method: The IC50 values of the aqueous, methanol, and acetone extracts against HCT 116 cells as well as the acetone extract against human colon fibroblast cell line CCD-18co were determined using the MTT assay. The concentration of the extracts ranged from 12.5 to 200μg/ml at treatment time of 24, 48 and 72h. Annexin V-FITC/PI labelling assay was employed to determine mode of HCT 116 cell death induced by acetone extract at 48h. The DNA damage induced by the extract in HCT 116 cells was detected using alkaline comet assay at 30min of IC10 and IC25 treatment. Results: Acetone extract exhibited the highest cytotoxic effect against HCT 116 cells compared to methanol and aqueous extract at 24, 48 and 72h. Despite no cytotoxic effect by acetone extract against CCD-18co cells at 24 and 48h, however at 72h, CCD-18co cells proliferated. Apoptosis assessment using Annexin V-FITC/PI labelling assay revealed that the primary cell death was via apoptosis after 48h treatment. Low doses of acetone extract from stem bark of C. odontophyllum showed significant DNA damage in HCT 116 cells with tail moment of 6.187 ± 0.718 A.U and 7.877 ± 0.142 A.U, respectively. Conclusions: Acetone extract from stem bark of C. odontophyllum has high potential in the development of anticancer agent against HCT 116 cells with no cytotoxic effect against human colon fibroblast cells.",
keywords = "Apoptosis, Canarium odontophyllum, CCD-18co, Colorectal cancer, Cytotoxicity, Dabai, DNA damage, HCT 116",
author = "Basri, {Dayang Fredalina} and Alamin, {Zafira Ayushah Zainul} and {Kok Meng}, Chan",
year = "2016",
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doi = "10.1186/s12906-016-1015-2",
language = "English",
volume = "16",
journal = "BMC Complementary and Alternative Medicine",
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T1 - Assessment of cytotoxicity and genotoxicity of stem bark extracts from Canarium odontophyllum Miq. (dabai) against HCT 116 human colorectal cancer cell line

AU - Basri, Dayang Fredalina

AU - Alamin, Zafira Ayushah Zainul

AU - Kok Meng, Chan

PY - 2016/1/29

Y1 - 2016/1/29

N2 - Background: Canarium odontophyllum Miq. is a plant species widely known as 'dabai' and can be vastly found in Sarawak. The aim of this study was to assess the cytotoxic and genotoxic effect of extracts from stem bark of C. odontophyllum against HCT 116 human colorectal cancer cell line. Method: The IC50 values of the aqueous, methanol, and acetone extracts against HCT 116 cells as well as the acetone extract against human colon fibroblast cell line CCD-18co were determined using the MTT assay. The concentration of the extracts ranged from 12.5 to 200μg/ml at treatment time of 24, 48 and 72h. Annexin V-FITC/PI labelling assay was employed to determine mode of HCT 116 cell death induced by acetone extract at 48h. The DNA damage induced by the extract in HCT 116 cells was detected using alkaline comet assay at 30min of IC10 and IC25 treatment. Results: Acetone extract exhibited the highest cytotoxic effect against HCT 116 cells compared to methanol and aqueous extract at 24, 48 and 72h. Despite no cytotoxic effect by acetone extract against CCD-18co cells at 24 and 48h, however at 72h, CCD-18co cells proliferated. Apoptosis assessment using Annexin V-FITC/PI labelling assay revealed that the primary cell death was via apoptosis after 48h treatment. Low doses of acetone extract from stem bark of C. odontophyllum showed significant DNA damage in HCT 116 cells with tail moment of 6.187 ± 0.718 A.U and 7.877 ± 0.142 A.U, respectively. Conclusions: Acetone extract from stem bark of C. odontophyllum has high potential in the development of anticancer agent against HCT 116 cells with no cytotoxic effect against human colon fibroblast cells.

AB - Background: Canarium odontophyllum Miq. is a plant species widely known as 'dabai' and can be vastly found in Sarawak. The aim of this study was to assess the cytotoxic and genotoxic effect of extracts from stem bark of C. odontophyllum against HCT 116 human colorectal cancer cell line. Method: The IC50 values of the aqueous, methanol, and acetone extracts against HCT 116 cells as well as the acetone extract against human colon fibroblast cell line CCD-18co were determined using the MTT assay. The concentration of the extracts ranged from 12.5 to 200μg/ml at treatment time of 24, 48 and 72h. Annexin V-FITC/PI labelling assay was employed to determine mode of HCT 116 cell death induced by acetone extract at 48h. The DNA damage induced by the extract in HCT 116 cells was detected using alkaline comet assay at 30min of IC10 and IC25 treatment. Results: Acetone extract exhibited the highest cytotoxic effect against HCT 116 cells compared to methanol and aqueous extract at 24, 48 and 72h. Despite no cytotoxic effect by acetone extract against CCD-18co cells at 24 and 48h, however at 72h, CCD-18co cells proliferated. Apoptosis assessment using Annexin V-FITC/PI labelling assay revealed that the primary cell death was via apoptosis after 48h treatment. Low doses of acetone extract from stem bark of C. odontophyllum showed significant DNA damage in HCT 116 cells with tail moment of 6.187 ± 0.718 A.U and 7.877 ± 0.142 A.U, respectively. Conclusions: Acetone extract from stem bark of C. odontophyllum has high potential in the development of anticancer agent against HCT 116 cells with no cytotoxic effect against human colon fibroblast cells.

KW - Apoptosis

KW - Canarium odontophyllum

KW - CCD-18co

KW - Colorectal cancer

KW - Cytotoxicity

KW - Dabai

KW - DNA damage

KW - HCT 116

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DO - 10.1186/s12906-016-1015-2

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C2 - 26822971

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VL - 16

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