Assessment of antioxidant and cytotoxicity activities of saponin and crude extracts of chlorophytum borivilianum

Mehdi Farshad Ashraf, Maheran Abd Aziz, Johnson Stanslas, Ismanizan Ismail, Mihdzar Abdul Kadir

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The present paper focused on antioxidant and cytotoxicity assessment of crude and total saponin fraction of Chlorophytum borivilianum as an important medicinal plant. In this study, three different antioxidant activities (2,2-diphenyl-1-picrylhydrazyl radical scavenging (DPPH), ferrous ion chelating (FIC), and β-carotene bleaching (BCB) activity) of crude extract and total saponin fraction of C. borivilianum tubers were performed. Crude extract was found to possess higher free radical scavenging activity (ascorbic acid equivalents 2578 ± 111 mg AA/100 g) and bleaching activity (IC 50 = 0.7 mg mL-1), while total saponin fraction displayed higher ferrous ion chelating (EC50 = 1 mg mL-1). Cytotoxicity evaluation of crude extract and total saponin fraction against MCF-7, PC3, and HCT-116 cancer cell lines using 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) cell viability assay indicated a higher cytotoxicity activity of the crude extract than the total saponin fraction on all cell lines, being most effective and selective on MCF-7 human breast cancer cell line.

Original languageEnglish
Article number216894
JournalThe Scientific World Journal
Volume2013
DOIs
Publication statusPublished - 2013

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Saponins
Cytotoxicity
Complex Mixtures
antioxidant
Antioxidants
bleaching
Cells
cancer
Scavenging
Bleaching
Chelation
medicinal plant
ion
Cell Line
tuber
free radical
ascorbic acid
bromide
Ions
viability

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Medicine(all)

Cite this

Assessment of antioxidant and cytotoxicity activities of saponin and crude extracts of chlorophytum borivilianum. / Ashraf, Mehdi Farshad; Abd Aziz, Maheran; Stanslas, Johnson; Ismail, Ismanizan; Abdul Kadir, Mihdzar.

In: The Scientific World Journal, Vol. 2013, 216894, 2013.

Research output: Contribution to journalArticle

Ashraf, Mehdi Farshad ; Abd Aziz, Maheran ; Stanslas, Johnson ; Ismail, Ismanizan ; Abdul Kadir, Mihdzar. / Assessment of antioxidant and cytotoxicity activities of saponin and crude extracts of chlorophytum borivilianum. In: The Scientific World Journal. 2013 ; Vol. 2013.
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