Arabidopsis AT2G02870 loss of function mutants lead to enhanced production of hydroperoxide lyase pathway genes and products

Muhammad Naeem-ul-Hassan, Zamri Zainal, Ismanizan Ismail, Nur Athirah Abd Hamid, Muhammad Sajad

Research output: Contribution to journalArticle

Abstract

F-box proteins containing variable C-terminal domains make an essential part of SKP1-Cullin-Ring box-F box (SCF) complex. SCF complex catalyzes the final step to link the ubiquitin tag with the target protein, destined for degradation, through F-box protein that confer overall substrate specificity to the complex. In this study, we analyzed the role of At2g02870, a Kelch containing F-box protein from Arabidopsis thaliana, by using reverse genetics strategy. At2g02870 loss of function mutant lines (at2g02870) were analyzed and compared with wild type plants for the expression of genes and products of hydroperoxide lyase (HPL) branch of oxylipin pathway. We found that the at2g02870 plants have enhanced expression of HPL pathway genes and produce more green leaf volatiles (GLV) than the wild type plants. Our results suggested that the gene is involved in the regulation of HPL pathway, possibly through the degradation of enzymes or/ and the regulatory factors of the pathway.

Original languageEnglish
Pages (from-to)3003-3008
Number of pages6
JournalSains Malaysiana
Volume47
Issue number12
DOIs
Publication statusPublished - 1 Dec 2018

Fingerprint

F-box proteins
lyases
hydroperoxides
Arabidopsis
mutants
oxylipins
genes
ubiquitin
substrate specificity
protein degradation
Arabidopsis thaliana
gene expression
degradation
enzymes
leaves

Keywords

  • F-box proteins
  • Hydroperoxide lyase
  • Kelch-repeat
  • Lipoxygenase
  • Oxylipin

ASJC Scopus subject areas

  • General

Cite this

Arabidopsis AT2G02870 loss of function mutants lead to enhanced production of hydroperoxide lyase pathway genes and products. / Naeem-ul-Hassan, Muhammad; Zainal, Zamri; Ismail, Ismanizan; Hamid, Nur Athirah Abd; Sajad, Muhammad.

In: Sains Malaysiana, Vol. 47, No. 12, 01.12.2018, p. 3003-3008.

Research output: Contribution to journalArticle

Naeem-ul-Hassan, Muhammad ; Zainal, Zamri ; Ismail, Ismanizan ; Hamid, Nur Athirah Abd ; Sajad, Muhammad. / Arabidopsis AT2G02870 loss of function mutants lead to enhanced production of hydroperoxide lyase pathway genes and products. In: Sains Malaysiana. 2018 ; Vol. 47, No. 12. pp. 3003-3008.
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