Aquilaria malaccensis polyploids as improved planting materials

A. R Siti Suhaila, M. S. Norihan, M. Norwati, M. A Nor Azah, M. C. Mahani, N. Parameswari, I. K. Kodi, J. Mailina, A. Azrina, H. Nor Hasnida, I. Haliza, A. Nazirah, Y. Muhd Fuad

    Research output: Contribution to journalArticle

    1 Citation (Scopus)

    Abstract

    Aquilaria malaccensis is an agarwood-producing timber species used in many traditional remedies and modern therapeutic treatments and perfume industries. In this study, we aimed to enhance A. malaccensis phytochemical content through in-vitro polyploidisation. Shoot tip and nodal segment from 8-week-old in-vitro A. malaccensis plantlets were treated with different concentrations of colchicine and trifluralin at various exposure times to obtain polyploids. Tetraploid plantlets (10%) was obtained using nodal segment explants treated with 0.1 mM trifluralin at 120 hours. Chemical profiling of diploid and tetraploid samples (leaf, stem and root) was evaluated separately using headspace-solid phase microextraction (HS-SPME) combined with gas chromatograph mass spectrometry (GCMS). Phytochemical content increased in tetraploid, particularly in stem whereby the total phytochemical contents were 43.19% in tetraploid compared with 5.87% in diploid. The HS-SPME-GCMS analyses showed that tetraploid stem contained high levels of sesquiterpenoids found in agarwood oil such as α-eudesmol (18.3%), α-gurjunene (8.61%) and γ-gurjunene (6.22%). On the other hand, aromadendrene (2.49%) and α-humulene (3.38%) were detected in diploid samples. Tetraploid leaf samples were observed to contain α-humulene (3.79%) while diploid only contained (2E) tridecenol (19%). There were no significant differences between diploid and tetraploid in terms of total phytochemical content in root samples. Nevertheless, high sesquiterpenoid content, γ-gurjunene (14.0%), was detected in tetraploid sample while γ-muurolene (2.96%), in diploid. α-Guaiene content was higher in root samples of diploid (6.49%) than tetraploid (1.09%). These results demonstrated that tetraploid plantlets led to higher yield of total phytochemical content and might facilitate production of high quality A. malaccensis clones.

    Original languageEnglish
    Pages (from-to)376-387
    Number of pages12
    JournalJournal of Tropical Forest Science
    Volume27
    Issue number3
    Publication statusPublished - 2015

    Fingerprint

    Aquilaria malaccensis
    polyploidy
    tetraploidy
    trifluralin
    stem
    planting
    diploidy
    mass spectrometry
    phytopharmaceuticals
    gas
    timber
    clone
    plantlets
    humulene
    shoot
    sampling
    oil
    sesquiterpenoids
    industry
    stems

    Keywords

    • Colchicine
    • Phytochemical content
    • Polyploidisation
    • Trifluralin

    ASJC Scopus subject areas

    • Forestry

    Cite this

    Suhaila, A. R. S., Norihan, M. S., Norwati, M., Azah, M. A. N., Mahani, M. C., Parameswari, N., ... Fuad, Y. M. (2015). Aquilaria malaccensis polyploids as improved planting materials. Journal of Tropical Forest Science, 27(3), 376-387.

    Aquilaria malaccensis polyploids as improved planting materials. / Suhaila, A. R Siti; Norihan, M. S.; Norwati, M.; Azah, M. A Nor; Mahani, M. C.; Parameswari, N.; Kodi, I. K.; Mailina, J.; Azrina, A.; Hasnida, H. Nor; Haliza, I.; Nazirah, A.; Fuad, Y. Muhd.

    In: Journal of Tropical Forest Science, Vol. 27, No. 3, 2015, p. 376-387.

    Research output: Contribution to journalArticle

    Suhaila, ARS, Norihan, MS, Norwati, M, Azah, MAN, Mahani, MC, Parameswari, N, Kodi, IK, Mailina, J, Azrina, A, Hasnida, HN, Haliza, I, Nazirah, A & Fuad, YM 2015, 'Aquilaria malaccensis polyploids as improved planting materials', Journal of Tropical Forest Science, vol. 27, no. 3, pp. 376-387.
    Suhaila ARS, Norihan MS, Norwati M, Azah MAN, Mahani MC, Parameswari N et al. Aquilaria malaccensis polyploids as improved planting materials. Journal of Tropical Forest Science. 2015;27(3):376-387.
    Suhaila, A. R Siti ; Norihan, M. S. ; Norwati, M. ; Azah, M. A Nor ; Mahani, M. C. ; Parameswari, N. ; Kodi, I. K. ; Mailina, J. ; Azrina, A. ; Hasnida, H. Nor ; Haliza, I. ; Nazirah, A. ; Fuad, Y. Muhd. / Aquilaria malaccensis polyploids as improved planting materials. In: Journal of Tropical Forest Science. 2015 ; Vol. 27, No. 3. pp. 376-387.
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    abstract = "Aquilaria malaccensis is an agarwood-producing timber species used in many traditional remedies and modern therapeutic treatments and perfume industries. In this study, we aimed to enhance A. malaccensis phytochemical content through in-vitro polyploidisation. Shoot tip and nodal segment from 8-week-old in-vitro A. malaccensis plantlets were treated with different concentrations of colchicine and trifluralin at various exposure times to obtain polyploids. Tetraploid plantlets (10{\%}) was obtained using nodal segment explants treated with 0.1 mM trifluralin at 120 hours. Chemical profiling of diploid and tetraploid samples (leaf, stem and root) was evaluated separately using headspace-solid phase microextraction (HS-SPME) combined with gas chromatograph mass spectrometry (GCMS). Phytochemical content increased in tetraploid, particularly in stem whereby the total phytochemical contents were 43.19{\%} in tetraploid compared with 5.87{\%} in diploid. The HS-SPME-GCMS analyses showed that tetraploid stem contained high levels of sesquiterpenoids found in agarwood oil such as α-eudesmol (18.3{\%}), α-gurjunene (8.61{\%}) and γ-gurjunene (6.22{\%}). On the other hand, aromadendrene (2.49{\%}) and α-humulene (3.38{\%}) were detected in diploid samples. Tetraploid leaf samples were observed to contain α-humulene (3.79{\%}) while diploid only contained (2E) tridecenol (19{\%}). There were no significant differences between diploid and tetraploid in terms of total phytochemical content in root samples. Nevertheless, high sesquiterpenoid content, γ-gurjunene (14.0{\%}), was detected in tetraploid sample while γ-muurolene (2.96{\%}), in diploid. α-Guaiene content was higher in root samples of diploid (6.49{\%}) than tetraploid (1.09{\%}). These results demonstrated that tetraploid plantlets led to higher yield of total phytochemical content and might facilitate production of high quality A. malaccensis clones.",
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    AU - Mahani, M. C.

    AU - Parameswari, N.

    AU - Kodi, I. K.

    AU - Mailina, J.

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