Aqueous extract of Piper sarmentosum decreases atherosclerotic lesions in high cholesterolemic experimental rabbits

Adel A. Amran, Zaiton Zakaria, Faizah Othman, Srijit Das, Santhna Raj L, Nor Anita M M Anita Nordin

Research output: Contribution to journalArticle

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Abstract

Background: Piper sarmentosum (P.s) has flavonoid component in its leaves which has antioxidative effect. To date, its effect on atherosclerosis has not been studied histologically. Aim: The study aimed to investigate the effect of P.s on atherosclerotic changes in hypercholesterolemic rabbits. Methods: Forty two male New Zealand white rabbits were divided into seven groups. C- control group fed normal rabbit chow, CH- cholesterol diet (1% cholesterol), W1- 1 % cholesterol with water extract of P.s (62.5mg/kg), W2- 1 % cholesterol with water extract of P.s (125mg/kg), W3- 1 % cholesterol with water extract of P.s (250mg/kg), W4 - 1 % cholesterol with water extract of P.s (500mg/kg) and Smv - 1 % cholesterol supplemented with simvistatin drug (1.2mg/kg). All rabbits were treated for 10 weeks. Following 10 weeks of supplementation, the animals were sacrificed and the aortic tissue was taken for histological study. Results: Rabbits fed only with high cholesterol diet 1% cholesterol (CH) showed focal fatty streak lesions compared to the C group and 1 % cholesterol supplemented with simvistatin drug (Smv) group. Atherosclerotic lesions in the 1 % cholesterol group supplemented with P.s (500mg/kg) i.e.W4 group showed significant reduction (30 +/-6 %, p 0.05 ) in fatty streak compared to the high cholesterol group (85.6 +/- 4.1%) under Sudan IV stain. The atherosclerotic lesions under transmission electron microscope showed reduction in foam cells in the treatment groups compared to the CH groups. Conclusion: Administration of P.s extract has protective effect against atherosclerosis.

Original languageEnglish
Pages (from-to)44
Number of pages1
JournalLipids in Health and Disease
DOIs
Publication statusAccepted/In press - 30 Apr 2010

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Piper
Cholesterol
Rabbits
Water
Nutrition
Atherosclerosis
Pharmaceutical Preparations
Diet
Foam Cells
Flavonoids

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism
  • Biochemistry, medical
  • Clinical Biochemistry

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Aqueous extract of Piper sarmentosum decreases atherosclerotic lesions in high cholesterolemic experimental rabbits. / Amran, Adel A.; Zakaria, Zaiton; Othman, Faizah; Das, Srijit; Raj L, Santhna; Nordin, Nor Anita M M Anita.

In: Lipids in Health and Disease, 30.04.2010, p. 44.

Research output: Contribution to journalArticle

Amran, Adel A. ; Zakaria, Zaiton ; Othman, Faizah ; Das, Srijit ; Raj L, Santhna ; Nordin, Nor Anita M M Anita. / Aqueous extract of Piper sarmentosum decreases atherosclerotic lesions in high cholesterolemic experimental rabbits. In: Lipids in Health and Disease. 2010 ; pp. 44.
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abstract = "Background: Piper sarmentosum (P.s) has flavonoid component in its leaves which has antioxidative effect. To date, its effect on atherosclerosis has not been studied histologically. Aim: The study aimed to investigate the effect of P.s on atherosclerotic changes in hypercholesterolemic rabbits. Methods: Forty two male New Zealand white rabbits were divided into seven groups. C- control group fed normal rabbit chow, CH- cholesterol diet (1{\%} cholesterol), W1- 1 {\%} cholesterol with water extract of P.s (62.5mg/kg), W2- 1 {\%} cholesterol with water extract of P.s (125mg/kg), W3- 1 {\%} cholesterol with water extract of P.s (250mg/kg), W4 - 1 {\%} cholesterol with water extract of P.s (500mg/kg) and Smv - 1 {\%} cholesterol supplemented with simvistatin drug (1.2mg/kg). All rabbits were treated for 10 weeks. Following 10 weeks of supplementation, the animals were sacrificed and the aortic tissue was taken for histological study. Results: Rabbits fed only with high cholesterol diet 1{\%} cholesterol (CH) showed focal fatty streak lesions compared to the C group and 1 {\%} cholesterol supplemented with simvistatin drug (Smv) group. Atherosclerotic lesions in the 1 {\%} cholesterol group supplemented with P.s (500mg/kg) i.e.W4 group showed significant reduction (30 +/-6 {\%}, p 0.05 ) in fatty streak compared to the high cholesterol group (85.6 +/- 4.1{\%}) under Sudan IV stain. The atherosclerotic lesions under transmission electron microscope showed reduction in foam cells in the treatment groups compared to the CH groups. Conclusion: Administration of P.s extract has protective effect against atherosclerosis.",
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