Aqueous extract of Centella asiatica promotes corneal epithelium wound healing in vitro

Ruszymah Idrus, Shiplu Roy Chowdhury, Nur Azeanty Bt Abdul Manan, Ooi Sew Fong, Mohd Ilham Adenan, Aminuddin Bin Saim

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Ethnopharmacological relevance: Centella asiatica is a traditional herbal medicine that has been shown to have pharmacological effect on skin wound healing, and could be potential therapeutic agent for corneal epithelial wound healing. Aim of the study: This study was done to evaluate the effects of Centella asiatica on the proliferation and migration of rabbit corneal epithelial (RCE) cells in the in vitro wound healing model. Materials and methods: RCE cells were cultured with or without supplementation of Centella asiatica aqueous extract. Viability and proliferation of the RCE cells was determined by MTT assay and cell cycle was analyzed by flow cytometry. In vitro re-epithelization was studied by scratch assay and migration rate was evaluated quantitatively by image analyzer. Expression of corneal specific differentiation markers, CK12 and connexin 43, were studied via RT-PCR. Results: It was found that supplementation of Centella asiatica did not show any significant effect on the RCE cells proliferation at the concentration up to 500 ppm, while at the concentration of 1000 ppm significantly inhibited RCE cells proliferation (p < 0.05). However, at the concentration up to 62.5 ppm, RCE cells shows significant enhancement of migration rate compared to the control group (p < 0.05). It was also found that the supplementation of Centella asiatica aqueous extract did not alter the expression of differentiation markers and cell cycle. Conclusion: In conclusion, supplementation of Centella asiatica aqueous extract at low concentrations could be useful to promote corneal epithelium wound healing.

Original languageEnglish
Pages (from-to)333-338
Number of pages6
JournalJournal of Ethnopharmacology
Volume140
Issue number2
DOIs
Publication statusPublished - 27 Mar 2012

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Corneal Epithelium
Wound Healing
Centella
Epithelial Cells
Rabbits
Differentiation Antigens
Cell Cycle
Cell Proliferation
Connexin 43
Herbal Medicine
Traditional Medicine
Centella asiatica extract
In Vitro Techniques
Flow Cytometry
Pharmacology
Polymerase Chain Reaction
Control Groups
Skin

Keywords

  • Centella asiatica
  • CK12
  • Connexin 43
  • Corneal epithelial cell
  • Corneal wound healing

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery

Cite this

Aqueous extract of Centella asiatica promotes corneal epithelium wound healing in vitro. / Idrus, Ruszymah; Chowdhury, Shiplu Roy; Manan, Nur Azeanty Bt Abdul; Fong, Ooi Sew; Adenan, Mohd Ilham; Saim, Aminuddin Bin.

In: Journal of Ethnopharmacology, Vol. 140, No. 2, 27.03.2012, p. 333-338.

Research output: Contribution to journalArticle

Idrus, Ruszymah ; Chowdhury, Shiplu Roy ; Manan, Nur Azeanty Bt Abdul ; Fong, Ooi Sew ; Adenan, Mohd Ilham ; Saim, Aminuddin Bin. / Aqueous extract of Centella asiatica promotes corneal epithelium wound healing in vitro. In: Journal of Ethnopharmacology. 2012 ; Vol. 140, No. 2. pp. 333-338.
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