Aqueous calyxes extract of Roselle or Hibiscus sabdariffa Linn supplementation improves liver morphology in streptozotocin induced diabetic rats

Akhtar Husin Nazratun Nafizah, Siti Balkis Budin, Abd Hamid Zaryantey, Abd Rahman Mariati, Raj Louis Santhana, Mohamad Osman, Md Idris Muhd Hanis, Jamaludin Mohamed

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Background and study aims: The complex series of deleterious events among diabetes patients leads to multiple organ failure. Therefore, a holistic approach of treatment is urgently required to prevent worsening of complications. The present investigation was carried out to study the possible protective effects of Roselle or Hibiscus sabdariffa Linn (HSL) calyxes aqueous extract, as an antidiabetic and antioxidant agent against oxidative liver injury in streptozotocin-induced diabetic rats. Material and methods: A single dose of streptozotocin (45. mg/kg body weight, iv) was used to induced diabetes in male Sprague Dawley rats which were then divided into two groups: Diabetic control (DC) and HSL-treated diabetic (DR) group. Normal rats were divided into normal control (NC), HSL-treated control (NR). Aqueous calyxes extract of HSL (100. mg/kg/day, orally) was given for 28 consecutive days in the treated group. Weight, biochemical and histopathological (light and electron microscopic) parameters were compared in all groups. Results: Supplementation of HSL significantly lowered the level of fasting blood glucose and increased plasma insulin level in DR group compared to DC group (p. <. 0.05). Alanine aminotransaminases and aspartate aminotransferase enzymes level were found to be significantly reduced in DR compared to DC. Microscopic examination demonstrated destruction of the liver architecture, cytoplasmic vacuolation of the hepatocytes and signs of necrosis in diabetic rats. Moreover, dilatation and congestion of blood vessels with leucocytes adherence were detected. Ultrastructural study using electron microscope showed homogeneous substance accumulation in nuclear chromatin, a decrease of organelles and mitochondrial degeneration in the diabetic rats. Conclusion: Administration of HSL in diabetic rats causes significant decrease in hepatocyte destruction and prevented the changes associated with the diabetic condition. Thus, our findings provide a scientific rationale for the use of HSL as promising agent in preventing liver injury in diabetes.

Original languageEnglish
JournalArab Journal of Gastroenterology
DOIs
Publication statusAccepted/In press - 19 Oct 2015

Fingerprint

Hibiscus
Streptozocin
Liver
Hepatocytes
Electrons
Control Groups
Multiple Organ Failure
Wounds and Injuries
Aspartate Aminotransferases
Alanine Transaminase
Hypoglycemic Agents
Organelles
Chromatin
Blood Vessels
Sprague Dawley Rats
Blood Glucose
Dilatation
Fasting
Leukocytes
Necrosis

Keywords

  • Diabetes mellitus
  • Hibiscus sabdariffa Linn
  • Liver histopathology
  • Liver morphology
  • Liver ultrastructure

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Aqueous calyxes extract of Roselle or Hibiscus sabdariffa Linn supplementation improves liver morphology in streptozotocin induced diabetic rats. / Nazratun Nafizah, Akhtar Husin; Budin, Siti Balkis; Zaryantey, Abd Hamid; Mariati, Abd Rahman; Santhana, Raj Louis; Osman, Mohamad; Muhd Hanis, Md Idris; Mohamed, Jamaludin.

In: Arab Journal of Gastroenterology, 19.10.2015.

Research output: Contribution to journalArticle

Nazratun Nafizah, Akhtar Husin ; Budin, Siti Balkis ; Zaryantey, Abd Hamid ; Mariati, Abd Rahman ; Santhana, Raj Louis ; Osman, Mohamad ; Muhd Hanis, Md Idris ; Mohamed, Jamaludin. / Aqueous calyxes extract of Roselle or Hibiscus sabdariffa Linn supplementation improves liver morphology in streptozotocin induced diabetic rats. In: Arab Journal of Gastroenterology. 2015.
@article{2396d316bcde485b9d223a0282515a5b,
title = "Aqueous calyxes extract of Roselle or Hibiscus sabdariffa Linn supplementation improves liver morphology in streptozotocin induced diabetic rats",
abstract = "Background and study aims: The complex series of deleterious events among diabetes patients leads to multiple organ failure. Therefore, a holistic approach of treatment is urgently required to prevent worsening of complications. The present investigation was carried out to study the possible protective effects of Roselle or Hibiscus sabdariffa Linn (HSL) calyxes aqueous extract, as an antidiabetic and antioxidant agent against oxidative liver injury in streptozotocin-induced diabetic rats. Material and methods: A single dose of streptozotocin (45. mg/kg body weight, iv) was used to induced diabetes in male Sprague Dawley rats which were then divided into two groups: Diabetic control (DC) and HSL-treated diabetic (DR) group. Normal rats were divided into normal control (NC), HSL-treated control (NR). Aqueous calyxes extract of HSL (100. mg/kg/day, orally) was given for 28 consecutive days in the treated group. Weight, biochemical and histopathological (light and electron microscopic) parameters were compared in all groups. Results: Supplementation of HSL significantly lowered the level of fasting blood glucose and increased plasma insulin level in DR group compared to DC group (p. <. 0.05). Alanine aminotransaminases and aspartate aminotransferase enzymes level were found to be significantly reduced in DR compared to DC. Microscopic examination demonstrated destruction of the liver architecture, cytoplasmic vacuolation of the hepatocytes and signs of necrosis in diabetic rats. Moreover, dilatation and congestion of blood vessels with leucocytes adherence were detected. Ultrastructural study using electron microscope showed homogeneous substance accumulation in nuclear chromatin, a decrease of organelles and mitochondrial degeneration in the diabetic rats. Conclusion: Administration of HSL in diabetic rats causes significant decrease in hepatocyte destruction and prevented the changes associated with the diabetic condition. Thus, our findings provide a scientific rationale for the use of HSL as promising agent in preventing liver injury in diabetes.",
keywords = "Diabetes mellitus, Hibiscus sabdariffa Linn, Liver histopathology, Liver morphology, Liver ultrastructure",
author = "{Nazratun Nafizah}, {Akhtar Husin} and Budin, {Siti Balkis} and Zaryantey, {Abd Hamid} and Mariati, {Abd Rahman} and Santhana, {Raj Louis} and Mohamad Osman and {Muhd Hanis}, {Md Idris} and Jamaludin Mohamed",
year = "2015",
month = "10",
day = "19",
doi = "10.1016/j.ajg.2017.02.001",
language = "English",
journal = "Arab Journal of Gastroenterology",
issn = "1687-1979",
publisher = "Elsevier BV",

}

TY - JOUR

T1 - Aqueous calyxes extract of Roselle or Hibiscus sabdariffa Linn supplementation improves liver morphology in streptozotocin induced diabetic rats

AU - Nazratun Nafizah, Akhtar Husin

AU - Budin, Siti Balkis

AU - Zaryantey, Abd Hamid

AU - Mariati, Abd Rahman

AU - Santhana, Raj Louis

AU - Osman, Mohamad

AU - Muhd Hanis, Md Idris

AU - Mohamed, Jamaludin

PY - 2015/10/19

Y1 - 2015/10/19

N2 - Background and study aims: The complex series of deleterious events among diabetes patients leads to multiple organ failure. Therefore, a holistic approach of treatment is urgently required to prevent worsening of complications. The present investigation was carried out to study the possible protective effects of Roselle or Hibiscus sabdariffa Linn (HSL) calyxes aqueous extract, as an antidiabetic and antioxidant agent against oxidative liver injury in streptozotocin-induced diabetic rats. Material and methods: A single dose of streptozotocin (45. mg/kg body weight, iv) was used to induced diabetes in male Sprague Dawley rats which were then divided into two groups: Diabetic control (DC) and HSL-treated diabetic (DR) group. Normal rats were divided into normal control (NC), HSL-treated control (NR). Aqueous calyxes extract of HSL (100. mg/kg/day, orally) was given for 28 consecutive days in the treated group. Weight, biochemical and histopathological (light and electron microscopic) parameters were compared in all groups. Results: Supplementation of HSL significantly lowered the level of fasting blood glucose and increased plasma insulin level in DR group compared to DC group (p. <. 0.05). Alanine aminotransaminases and aspartate aminotransferase enzymes level were found to be significantly reduced in DR compared to DC. Microscopic examination demonstrated destruction of the liver architecture, cytoplasmic vacuolation of the hepatocytes and signs of necrosis in diabetic rats. Moreover, dilatation and congestion of blood vessels with leucocytes adherence were detected. Ultrastructural study using electron microscope showed homogeneous substance accumulation in nuclear chromatin, a decrease of organelles and mitochondrial degeneration in the diabetic rats. Conclusion: Administration of HSL in diabetic rats causes significant decrease in hepatocyte destruction and prevented the changes associated with the diabetic condition. Thus, our findings provide a scientific rationale for the use of HSL as promising agent in preventing liver injury in diabetes.

AB - Background and study aims: The complex series of deleterious events among diabetes patients leads to multiple organ failure. Therefore, a holistic approach of treatment is urgently required to prevent worsening of complications. The present investigation was carried out to study the possible protective effects of Roselle or Hibiscus sabdariffa Linn (HSL) calyxes aqueous extract, as an antidiabetic and antioxidant agent against oxidative liver injury in streptozotocin-induced diabetic rats. Material and methods: A single dose of streptozotocin (45. mg/kg body weight, iv) was used to induced diabetes in male Sprague Dawley rats which were then divided into two groups: Diabetic control (DC) and HSL-treated diabetic (DR) group. Normal rats were divided into normal control (NC), HSL-treated control (NR). Aqueous calyxes extract of HSL (100. mg/kg/day, orally) was given for 28 consecutive days in the treated group. Weight, biochemical and histopathological (light and electron microscopic) parameters were compared in all groups. Results: Supplementation of HSL significantly lowered the level of fasting blood glucose and increased plasma insulin level in DR group compared to DC group (p. <. 0.05). Alanine aminotransaminases and aspartate aminotransferase enzymes level were found to be significantly reduced in DR compared to DC. Microscopic examination demonstrated destruction of the liver architecture, cytoplasmic vacuolation of the hepatocytes and signs of necrosis in diabetic rats. Moreover, dilatation and congestion of blood vessels with leucocytes adherence were detected. Ultrastructural study using electron microscope showed homogeneous substance accumulation in nuclear chromatin, a decrease of organelles and mitochondrial degeneration in the diabetic rats. Conclusion: Administration of HSL in diabetic rats causes significant decrease in hepatocyte destruction and prevented the changes associated with the diabetic condition. Thus, our findings provide a scientific rationale for the use of HSL as promising agent in preventing liver injury in diabetes.

KW - Diabetes mellitus

KW - Hibiscus sabdariffa Linn

KW - Liver histopathology

KW - Liver morphology

KW - Liver ultrastructure

UR - http://www.scopus.com/inward/record.url?scp=85015749768&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85015749768&partnerID=8YFLogxK

U2 - 10.1016/j.ajg.2017.02.001

DO - 10.1016/j.ajg.2017.02.001

M3 - Article

C2 - 28336227

AN - SCOPUS:85015749768

JO - Arab Journal of Gastroenterology

JF - Arab Journal of Gastroenterology

SN - 1687-1979

ER -