Approaches to deriving Schwann cells from human bone marrow for neural tube regeneration in a clinical setting.

H. Nur Hidayah, M. Mazzre, Min Hwei Ng, Ruszymah Idrus, Shalimar Abdullah

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6 Citations (Scopus)

Abstract

Bone marrow derived Mesenchymal stem cells (MSCs) were evaluated as an alternative source for tissue engineering of peripheral nerves. Human MSCs were subjected to a series of treatment with a reducing agent, retinoic acid and a combination of trophic factors. This treated MSCs differentiated into Schwann cells were characterized in vitro via flow cytometry analysis and immunocytochemically. In contrast to untreated MSCs, differentiated MSCs expressed Schwann cell markers in vitro, as we confirmed by flow cytometry analysis and immunocytochemically. These results suggest that human MSCs can be induced to be a substitute for Schwann cells that may be applied for nerve regeneration since it is difficult to grow Schwann cells in vitro.

Original languageEnglish
Pages (from-to)39-40
Number of pages2
JournalThe Medical journal of Malaysia
Volume63 Suppl A
Publication statusPublished - Jul 2008

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Neural Tube
Schwann Cells
Mesenchymal Stromal Cells
Regeneration
Bone Marrow
Flow Cytometry
Nerve Regeneration
Reducing Agents
Tissue Engineering
Tretinoin
Peripheral Nerves
In Vitro Techniques

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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AU - Mazzre, M.

AU - Ng, Min Hwei

AU - Idrus, Ruszymah

AU - Abdullah, Shalimar

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AB - Bone marrow derived Mesenchymal stem cells (MSCs) were evaluated as an alternative source for tissue engineering of peripheral nerves. Human MSCs were subjected to a series of treatment with a reducing agent, retinoic acid and a combination of trophic factors. This treated MSCs differentiated into Schwann cells were characterized in vitro via flow cytometry analysis and immunocytochemically. In contrast to untreated MSCs, differentiated MSCs expressed Schwann cell markers in vitro, as we confirmed by flow cytometry analysis and immunocytochemically. These results suggest that human MSCs can be induced to be a substitute for Schwann cells that may be applied for nerve regeneration since it is difficult to grow Schwann cells in vitro.

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