Applications of the Box-Wilson design model for bio-hydrogen production using clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564)

W. M. Alalayah, Mohd. Sahaid Kalil, Abdul Amir H. Kadhum, Jamaliah Md Jahim, Azami Zaharim, N. M. Alauj, A. El-Shafie

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Abstract

Box-Wilson Design (BWD) model was applied to determine the optimum values of influencing parameters in anaerobic fermentation to produce hydrogen using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564). The main focus of the study was to find the optimal relationship between the hydrogen yield and three variables including initial substrate concentration, initial medium pH and reaction temperature. Microbial growth kinetic parameters for hydrogen production under anaerobic conditions were determined using the Monod model with incorporation of a substrate inhibition term. The values of μmax (maximum specific growth rate) and Ks (saturation constant) were 0.398 h·1 and 5.509 g L·1, respectively, using glucose as the substrate. The experimental substrate and biomass-concentration profiles were in good agreement with those obtained by the kinetic-model predictions. By varying the conditions of the initial substrate concentration (1-40 g L·1), reaction temperature (25-40°C) and initial medium pH (4-8), the model predicted a maximum hydrogen yield of 3.24 mol H 2 (mol glucose)·1. The experimental data collected utilising this design was successfully fitted to a second-order polynomial model. An optimum operating condition of 10 g L·1 initial substrate concentration, 37°C reaction temperature and 6.0±0.2 initial medium pH gave 80% of the predicted maximum yield of hydrogen where as the experimental yield obtained in this study was 77.75% exhibiting a close accuracy between estimated and experimental values. This is the first report to predict bio-hydrogen yield by applying Box-Wilson Design in anaerobic fermentation while optimizing the effects of environmental factors prevailing there by investigating the effects of environmental factors.

Original languageEnglish
Pages (from-to)674-682
Number of pages9
JournalPakistan Journal of Biological Sciences
Volume13
Issue number14
DOIs
Publication statusPublished - 2010

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Clostridium saccharoperbutylacetonicum
hydrogen production
hydrogen
fermentation
kinetics
temperature
glucose
environmental factors
anaerobic conditions
specific growth rate
microbial growth
prediction
biomass

Keywords

  • Anaerobic fermentation
  • Bio-hydrogen production
  • Box-Wilson design model
  • Renewable energy

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

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title = "Applications of the Box-Wilson design model for bio-hydrogen production using clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564)",
abstract = "Box-Wilson Design (BWD) model was applied to determine the optimum values of influencing parameters in anaerobic fermentation to produce hydrogen using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564). The main focus of the study was to find the optimal relationship between the hydrogen yield and three variables including initial substrate concentration, initial medium pH and reaction temperature. Microbial growth kinetic parameters for hydrogen production under anaerobic conditions were determined using the Monod model with incorporation of a substrate inhibition term. The values of μmax (maximum specific growth rate) and Ks (saturation constant) were 0.398 h·1 and 5.509 g L·1, respectively, using glucose as the substrate. The experimental substrate and biomass-concentration profiles were in good agreement with those obtained by the kinetic-model predictions. By varying the conditions of the initial substrate concentration (1-40 g L·1), reaction temperature (25-40°C) and initial medium pH (4-8), the model predicted a maximum hydrogen yield of 3.24 mol H 2 (mol glucose)·1. The experimental data collected utilising this design was successfully fitted to a second-order polynomial model. An optimum operating condition of 10 g L·1 initial substrate concentration, 37°C reaction temperature and 6.0±0.2 initial medium pH gave 80{\%} of the predicted maximum yield of hydrogen where as the experimental yield obtained in this study was 77.75{\%} exhibiting a close accuracy between estimated and experimental values. This is the first report to predict bio-hydrogen yield by applying Box-Wilson Design in anaerobic fermentation while optimizing the effects of environmental factors prevailing there by investigating the effects of environmental factors.",
keywords = "Anaerobic fermentation, Bio-hydrogen production, Box-Wilson design model, Renewable energy",
author = "Alalayah, {W. M.} and Kalil, {Mohd. Sahaid} and Kadhum, {Abdul Amir H.} and {Md Jahim}, Jamaliah and Azami Zaharim and Alauj, {N. M.} and A. El-Shafie",
year = "2010",
doi = "10.3923/pjbs.2010.674.682",
language = "English",
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T1 - Applications of the Box-Wilson design model for bio-hydrogen production using clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564)

AU - Alalayah, W. M.

AU - Kalil, Mohd. Sahaid

AU - Kadhum, Abdul Amir H.

AU - Md Jahim, Jamaliah

AU - Zaharim, Azami

AU - Alauj, N. M.

AU - El-Shafie, A.

PY - 2010

Y1 - 2010

N2 - Box-Wilson Design (BWD) model was applied to determine the optimum values of influencing parameters in anaerobic fermentation to produce hydrogen using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564). The main focus of the study was to find the optimal relationship between the hydrogen yield and three variables including initial substrate concentration, initial medium pH and reaction temperature. Microbial growth kinetic parameters for hydrogen production under anaerobic conditions were determined using the Monod model with incorporation of a substrate inhibition term. The values of μmax (maximum specific growth rate) and Ks (saturation constant) were 0.398 h·1 and 5.509 g L·1, respectively, using glucose as the substrate. The experimental substrate and biomass-concentration profiles were in good agreement with those obtained by the kinetic-model predictions. By varying the conditions of the initial substrate concentration (1-40 g L·1), reaction temperature (25-40°C) and initial medium pH (4-8), the model predicted a maximum hydrogen yield of 3.24 mol H 2 (mol glucose)·1. The experimental data collected utilising this design was successfully fitted to a second-order polynomial model. An optimum operating condition of 10 g L·1 initial substrate concentration, 37°C reaction temperature and 6.0±0.2 initial medium pH gave 80% of the predicted maximum yield of hydrogen where as the experimental yield obtained in this study was 77.75% exhibiting a close accuracy between estimated and experimental values. This is the first report to predict bio-hydrogen yield by applying Box-Wilson Design in anaerobic fermentation while optimizing the effects of environmental factors prevailing there by investigating the effects of environmental factors.

AB - Box-Wilson Design (BWD) model was applied to determine the optimum values of influencing parameters in anaerobic fermentation to produce hydrogen using Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564). The main focus of the study was to find the optimal relationship between the hydrogen yield and three variables including initial substrate concentration, initial medium pH and reaction temperature. Microbial growth kinetic parameters for hydrogen production under anaerobic conditions were determined using the Monod model with incorporation of a substrate inhibition term. The values of μmax (maximum specific growth rate) and Ks (saturation constant) were 0.398 h·1 and 5.509 g L·1, respectively, using glucose as the substrate. The experimental substrate and biomass-concentration profiles were in good agreement with those obtained by the kinetic-model predictions. By varying the conditions of the initial substrate concentration (1-40 g L·1), reaction temperature (25-40°C) and initial medium pH (4-8), the model predicted a maximum hydrogen yield of 3.24 mol H 2 (mol glucose)·1. The experimental data collected utilising this design was successfully fitted to a second-order polynomial model. An optimum operating condition of 10 g L·1 initial substrate concentration, 37°C reaction temperature and 6.0±0.2 initial medium pH gave 80% of the predicted maximum yield of hydrogen where as the experimental yield obtained in this study was 77.75% exhibiting a close accuracy between estimated and experimental values. This is the first report to predict bio-hydrogen yield by applying Box-Wilson Design in anaerobic fermentation while optimizing the effects of environmental factors prevailing there by investigating the effects of environmental factors.

KW - Anaerobic fermentation

KW - Bio-hydrogen production

KW - Box-Wilson design model

KW - Renewable energy

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