Application of flow cytometry for estimation of nuclear dna content in elaeis

M. Madon, L. Q. Phoon, M. M. Clyde, Din A. Mohd

    Research output: Contribution to journalArticle

    13 Citations (Scopus)

    Abstract

    A method was established and proposed to he the standard protocol for estimating the genome size of oil palm LElaeis guineensis and its intraspecific hybrids, E. oleifera and oleifera x guineensis (Ox C) interspecific hybrids]. The method used is laser-sourced flow cytometry. Oil palm Frond -1 leaflets, LBOI lysis buffer and Glycine max cv. Polanka (as external standard) were used for the DNA content estimation. The 2C DNA contents of E. guineensis for the different fruit types were: dura (D)-4.10 ± 0.20, pisifera (P)-3.64 ± 0.28 and tenera (DxP)-3.83 ± 0.31 pg. A simple average gave the DNA content forE. guineensis as 3.86 ±0.26 pg.

    Original languageEnglish
    Pages (from-to)447-452
    Number of pages6
    JournalJournal of Oil Palm Research
    Volume20
    Issue numberJUNE
    Publication statusPublished - Jun 2008

    Fingerprint

    Elaeis
    Flow cytometry
    flow cytometry
    Flow Cytometry
    DNA
    Palm oil
    Elaeis guineensis
    Oils
    Genome Size
    oxen
    fronds
    Fruits
    Soybeans
    Glycine
    lasers
    Amino acids
    Glycine max
    Fruit
    Buffers
    Lasers

    Keywords

    • DNA content
    • Elaeis guineensis
    • Elaeis oleifera
    • Flow cyometry
    • Intra- and interspecific hybrids

    ASJC Scopus subject areas

    • Agronomy and Crop Science
    • Food Science
    • Renewable Energy, Sustainability and the Environment
    • Biomaterials

    Cite this

    Madon, M., Phoon, L. Q., Clyde, M. M., & Mohd, D. A. (2008). Application of flow cytometry for estimation of nuclear dna content in elaeis. Journal of Oil Palm Research, 20(JUNE), 447-452.

    Application of flow cytometry for estimation of nuclear dna content in elaeis. / Madon, M.; Phoon, L. Q.; Clyde, M. M.; Mohd, Din A.

    In: Journal of Oil Palm Research, Vol. 20, No. JUNE, 06.2008, p. 447-452.

    Research output: Contribution to journalArticle

    Madon, M, Phoon, LQ, Clyde, MM & Mohd, DA 2008, 'Application of flow cytometry for estimation of nuclear dna content in elaeis', Journal of Oil Palm Research, vol. 20, no. JUNE, pp. 447-452.
    Madon, M. ; Phoon, L. Q. ; Clyde, M. M. ; Mohd, Din A. / Application of flow cytometry for estimation of nuclear dna content in elaeis. In: Journal of Oil Palm Research. 2008 ; Vol. 20, No. JUNE. pp. 447-452.
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    abstract = "A method was established and proposed to he the standard protocol for estimating the genome size of oil palm LElaeis guineensis and its intraspecific hybrids, E. oleifera and oleifera x guineensis (Ox C) interspecific hybrids]. The method used is laser-sourced flow cytometry. Oil palm Frond -1 leaflets, LBOI lysis buffer and Glycine max cv. Polanka (as external standard) were used for the DNA content estimation. The 2C DNA contents of E. guineensis for the different fruit types were: dura (D)-4.10 ± 0.20, pisifera (P)-3.64 ± 0.28 and tenera (DxP)-3.83 ± 0.31 pg. A simple average gave the DNA content forE. guineensis as 3.86 ±0.26 pg.",
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