Apoptosis and cell cycle effect of Lignosus rhinocerus extract on HCT 116 human colorectal cancer cells

Suziana Zaila Che Fauzi, Nor Fadilah Rajab, Lek Mun Leong, Kok Lun Pang, Norfazlina Mohd Nawi, Nurshahirah Nasir, Florinsiah Lorin, Farida Zuraina Mohd Yusof

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Lignosus rhinocerus, locally known in Malaysia as cendawan susu rimau or tiger milk mushroom, belongs to Polyporaceae family. The mushroom is traditionally used in Malaysia to improve health status as well as cure for cough, asthma, fever, chronic hepatitis and cancer. In this study, pressurized liquid extraction was performed to L. rhinocerus and cellular effects of L. rhinocerus methanol extract(LRME)and aqueous extract (LRAE)were assessed in HCT 116 human colorectal carcinoma cells. LRME exerted higher cytotoxic activity (IC<inf>50</inf>: 600 µg/ml) compared to LRAE (IC<inf>50</inf>: 1200 µg/ml) in HCT 116 cells as determined by MTT assay. Interestingly, both extracts did not exert cytotoxic activity on CCD-18-Co human normal colon fibroblast cells and V79 Chinese hamster lung fibroblast cells (IC<inf>50</inf>> 2000 µg/ml). LRME and LRAE effect on cell cycle and mode of cell death were then investigated in HCT 116 cells as targeted cells. The mode of cell death induced by LRME and LRAE was primarily apoptosis as assessed by Annexin V-FITC/PI dual staining. Under low dose, both extracts arrested HCT 116 cells at G<inf>0</inf>/G<inf>1</inf> phases with corresponding decrease of S-phase population. In conclusion, this is the first report of Lignosus rhinocerus possible mechanism of inducing anti-proliferation on HCT 116 cells.

Original languageEnglish
Article number03
Pages (from-to)13-17
Number of pages5
JournalInternational Journal of Pharmaceutical Sciences Review and Research
Volume33
Issue number1
Publication statusPublished - 1 Aug 2015

Fingerprint

HCT116 Cells
Methanol
Colorectal Neoplasms
Cell Cycle
Apoptosis
Inhibitory Concentration 50
Agaricales
Malaysia
Polyporaceae
Cell Death
Fibroblasts
Tigers
Cell Cycle Resting Phase
Fluorescein-5-isothiocyanate
Annexin A5
G1 Phase
Chronic Hepatitis
Cricetulus
S Phase
Cough

Keywords

  • Apoptosis
  • HCT 116 cells
  • Lignosus rhinocerus
  • Pressurized liquid extraction
  • Tiger milk mushroom

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Apoptosis and cell cycle effect of Lignosus rhinocerus extract on HCT 116 human colorectal cancer cells. / Fauzi, Suziana Zaila Che; Rajab, Nor Fadilah; Leong, Lek Mun; Pang, Kok Lun; Nawi, Norfazlina Mohd; Nasir, Nurshahirah; Lorin, Florinsiah; Yusof, Farida Zuraina Mohd.

In: International Journal of Pharmaceutical Sciences Review and Research, Vol. 33, No. 1, 03, 01.08.2015, p. 13-17.

Research output: Contribution to journalArticle

Fauzi, Suziana Zaila Che ; Rajab, Nor Fadilah ; Leong, Lek Mun ; Pang, Kok Lun ; Nawi, Norfazlina Mohd ; Nasir, Nurshahirah ; Lorin, Florinsiah ; Yusof, Farida Zuraina Mohd. / Apoptosis and cell cycle effect of Lignosus rhinocerus extract on HCT 116 human colorectal cancer cells. In: International Journal of Pharmaceutical Sciences Review and Research. 2015 ; Vol. 33, No. 1. pp. 13-17.
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