Aortic remodelling in chronic nicotine-administered rat

Satirah Zainalabidin, Siti Balkis Budin, Anand Ramalingam, Yi Cheng Lim

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Vascular remodelling is an adaptive mechanism, which counteracts pressure changes in blood circulation. Nicotine content in cigarette increases the risk of hypertension. The exact relationship between nicotine and vascular remodelling still remain unknown. Current study was aimed to determine the effect of clinically relevant dosage of nicotine (equivalent to light smoker) on aortic reactivity, oxidative stress markers and histomorphological changes. Twelve age-matched male Sprague- Dawley rats were randomly divided into two groups, i.e.: normal saline as control or 0.6 mg/kg nicotine for 28 days (i.p., n=6 per group). On day-29, the rats were sacrificed and the thoracic aorta was dissected immediately for further studies. Mean arterial pressure (MAP) and pulse pressure (PP) of nicotine-treated vs. control were significantly increased (p< 0.05). Nicotine-treated group showed significant (p< 0.05) increase tunica media thickness, and decrease in lumen diameter, suggesting vascular remodelling which lead to prior hypertension state. The phenylephrine (PE)-induced contractile response in nicotine group was significantly higher than control group (ED50 = 1.44×105 M vs. 4.9×106 M) (p< 0.05 ∼ 0.001). However, nicotine-treated rat showed significantly lower endothelium-dependent relaxation response to acetylcholine (ACh) than in control group (ED50 = 6.17×107 M vs. 2.82×107 M) (p< 0.05), indicating loss of primary vascular function. Malondialdehyde (MDA), a lipid peroxidation marker was significantly higher in nicotine group. Superoxide dismutase (SOD) enzymatic activity and glutathione (GSH) were all reduced in nicotine group (p< 0.05) vs. control, suggesting nicotine induces oxidative imbalance. In short, chronic nicotine administration impaired aortic reactivity, probably via redox imbalance and vascular remodelling mechanism.

Original languageEnglish
Pages (from-to)411-418
Number of pages8
JournalKorean Journal of Physiology and Pharmacology
Volume18
Issue number5
DOIs
Publication statusPublished - 1 Oct 2014

Fingerprint

Nicotine
Hypertension
Tunica Media
Control Groups
Blood Circulation
Phenylephrine
Malondialdehyde
Thoracic Aorta
Tobacco Products
Lipid Peroxidation
Acetylcholine
Superoxide Dismutase
Oxidation-Reduction
Endothelium
Glutathione
Blood Vessels
Sprague Dawley Rats
Arterial Pressure
Oxidative Stress
Blood Pressure

Keywords

  • Aorta
  • Nicotine
  • Oxidative stress
  • Reactivity
  • Remodelling

ASJC Scopus subject areas

  • Pharmacology
  • Physiology

Cite this

Aortic remodelling in chronic nicotine-administered rat. / Zainalabidin, Satirah; Budin, Siti Balkis; Ramalingam, Anand; Lim, Yi Cheng.

In: Korean Journal of Physiology and Pharmacology, Vol. 18, No. 5, 01.10.2014, p. 411-418.

Research output: Contribution to journalArticle

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