Antiviral activity of Cynometra cauliflora leaves methanolic extract towards dengue virus type 2

Noor Zarina Abd Wahab, Norhidayah Badya, Nazlina Ibrahim, Mohd Khairul Amri Kamarudin, Hafizan Juahir, Mohd. Ekhwan Toriman

Research output: Contribution to journalArticle

Abstract

The present study is aimed at determining cytotoxicity and antiviral activities for methanolic extract obtained from leaves of Cynometra cauliflora. Cytotoxicity screening against Vero cells using MTT assay showed that the CC50 values for the extract was 36 mg/ml and the 50% Effective Concentration, EC50, was 2.19 mg/ml. The antiviral activity towards dengue virus type 2 (DENV-2) was determined us-ing MTT method. Three treatments were used in the antiviral test; 1) post-treatment, 2) pre-treatment, and 3) virucidal. The results re-vealed that the post-treatment was more effective in inhibiting viral replication compared to pre-treatment and virucidal assay. The selec-tivity index (SI = CC50 / EC50) for the extract was 16. The findings indicated that the extract prepared from C. cauliflora was non cyto-toxic to the cell with potential antiviral activity.

Original languageEnglish
Pages (from-to)344-347
Number of pages4
JournalInternational Journal of Engineering and Technology(UAE)
Volume7
Issue number3.14 Special Issue 14
Publication statusPublished - 1 Jan 2018

Fingerprint

Dengue Virus
Cytotoxicity
Viruses
Antiviral Agents
Assays
Screening
Vero Cells
Poisons

Keywords

  • Antiviral
  • Antiviral activity
  • Cynometra cauliflora
  • Dengue virus
  • Methanol extract

ASJC Scopus subject areas

  • Biotechnology
  • Computer Science (miscellaneous)
  • Environmental Engineering
  • Chemical Engineering(all)
  • Engineering(all)
  • Hardware and Architecture

Cite this

Antiviral activity of Cynometra cauliflora leaves methanolic extract towards dengue virus type 2. / Abd Wahab, Noor Zarina; Badya, Norhidayah; Ibrahim, Nazlina; Kamarudin, Mohd Khairul Amri; Juahir, Hafizan; Toriman, Mohd. Ekhwan.

In: International Journal of Engineering and Technology(UAE), Vol. 7, No. 3.14 Special Issue 14, 01.01.2018, p. 344-347.

Research output: Contribution to journalArticle

Abd Wahab, Noor Zarina ; Badya, Norhidayah ; Ibrahim, Nazlina ; Kamarudin, Mohd Khairul Amri ; Juahir, Hafizan ; Toriman, Mohd. Ekhwan. / Antiviral activity of Cynometra cauliflora leaves methanolic extract towards dengue virus type 2. In: International Journal of Engineering and Technology(UAE). 2018 ; Vol. 7, No. 3.14 Special Issue 14. pp. 344-347.
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