Antioxidant capacities and total phenolic contents increase with gamma irradiation in two types of Malaysian honey

Saba Zuhair Hussein, Kamaruddin Mohd Yusoff, Suzana Makpol, Yasmin Anum Mohd Yusof

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Two types of monofloral Malaysian honey (Gelam and Nenas) were analyzed to determine their antioxidant activities and total phenolic and flavonoid contents, with and without gamma irradiation. Our results showed that both types of honey can scavenge free radicals and exhibit high antioxidant-reducing power; however, Gelam honey exhibited higher antioxidant activity (p < 0.05) than Nenas honey, which is in good correlation (r = 0.9899) with its phenolic contents. Interestingly, we also noted that both irradiated honeys have higher antioxidant activities and total phenolic and flavonoid contents compared to nonirradiated honeys by Folin-Ciocalteu and UV-spectrophotometry methods, respectively. However, HPLC analysis for phenolic compounds showed insignificant increase between irradiated and nonirradiated honeys. The phenolic compounds such as: caffeic acid, chlorogenic acid, ellagic acid, p- coumaric acid, quercetin and hesperetin as indicated by HPLC method were found to be higher in Gelam honey versus Nenas honey. In conclusion, irradiation of honey causes enhanced antioxidant activities and flavonoid compounds.

Original languageEnglish
Pages (from-to)6378-6395
Number of pages18
JournalMolecules
Volume16
Issue number8
DOIs
Publication statusPublished - Aug 2011

Fingerprint

Honey
antioxidants
Antioxidants
Irradiation
irradiation
Flavonoids
acids
Ellagic Acid
Chlorogenic Acid
Quercetin
Spectrophotometry
spectrophotometry
free radicals
Free Radicals
High Pressure Liquid Chromatography
causes

Keywords

  • Antioxidant activity
  • Gamma irradiation
  • Gelam honey
  • Nenas honey
  • Phenolics

ASJC Scopus subject areas

  • Organic Chemistry

Cite this

Antioxidant capacities and total phenolic contents increase with gamma irradiation in two types of Malaysian honey. / Hussein, Saba Zuhair; Yusoff, Kamaruddin Mohd; Makpol, Suzana; Mohd Yusof, Yasmin Anum.

In: Molecules, Vol. 16, No. 8, 08.2011, p. 6378-6395.

Research output: Contribution to journalArticle

Hussein, Saba Zuhair ; Yusoff, Kamaruddin Mohd ; Makpol, Suzana ; Mohd Yusof, Yasmin Anum. / Antioxidant capacities and total phenolic contents increase with gamma irradiation in two types of Malaysian honey. In: Molecules. 2011 ; Vol. 16, No. 8. pp. 6378-6395.
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