Antioxidant, antimicrobial activity and in-vitro cytotoxicity screening study of pili nut oil

Z. Zarinah, Ma`Aruf Abd. Ghani, R. Nazaruddin, W. W W Wong, X. Xuebing

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Some vegetable oils contain natural antioxidants such as beta carotene and vitamin E namely alpha tocopherol. The objective of this study was to screening the value of α-tocopherol, β-carotene, antioxidant capacity, antimicrobial activity and toxicological properties of roasted pili nut oil (RPNO) and unroasted pili nut oil (UPNO). The result showed that RPNO contained higher amount of vitamin E and less amount of beta carotene compared to UPNO. RPNO and UPNO scavenged DPPH radicals by 24.66% and 9.52% at concentration of 140 μg/ml. The total phenolic compound (TPC) in UPNO and RPNO were about 19.96 ± 0.52 mg/kg and 12.43 ± 0.69 mg/kg respectively. It was observed that bacteria species exhibited different sensitivities towards RPNO, UPNO, Gentamycin, Ampicillin and Chloramphenicol. Bacillus cereus 14570 was the most sensitive bacterium and all strains of Staphylococcus aureus tested were resistant against both samples RPNO and UPNO. An in vitro toxicological study based on the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) cytotoxicity assay was also performed. In vitro cytotoxicity indicated that both RPNO and UPNO had no effect against HeLa (cervical cancer cell), MCF-7 (breast cancer cell) and HT-29 (human colon adenocarcinoma cell) cell lines tested.

Original languageEnglish
Pages (from-to)309-316
Number of pages8
JournalInternational Food Research Journal
Volume21
Issue number1
Publication statusPublished - 2014

Fingerprint

pili nuts
nut oils
Nuts
cytotoxicity
Oils
Antioxidants
anti-infective agents
screening
antioxidants
beta Carotene
In Vitro Techniques
Vitamin E
Toxicology
beta-carotene
vitamin E

Keywords

  • α-tocopherol β-carotene
  • Antimicrobial activity
  • Antioxidant
  • Canarium ovatum
  • Cytotoxicity

ASJC Scopus subject areas

  • Food Science

Cite this

Antioxidant, antimicrobial activity and in-vitro cytotoxicity screening study of pili nut oil. / Zarinah, Z.; Abd. Ghani, Ma`Aruf; Nazaruddin, R.; Wong, W. W W; Xuebing, X.

In: International Food Research Journal, Vol. 21, No. 1, 2014, p. 309-316.

Research output: Contribution to journalArticle

Zarinah, Z. ; Abd. Ghani, Ma`Aruf ; Nazaruddin, R. ; Wong, W. W W ; Xuebing, X. / Antioxidant, antimicrobial activity and in-vitro cytotoxicity screening study of pili nut oil. In: International Food Research Journal. 2014 ; Vol. 21, No. 1. pp. 309-316.
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