Antioxidant and antibacterial assays on polygonum minus extracts

Different extraction methods

Norsyamimi Hassim, Masturah Markom, Nurina Anuar, Kurnia Harlina Dewi, Syarul Nataqain Baharum, Normah Mohd. Noor

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10 Citations (Scopus)

Abstract

The effect of solvent type and extraction method was investigated to study the antioxidant and antibacterial activity of Polygonum minus. Two extraction methods were used: a solvent extraction using Soxhlet apparatus and supercritical fluid extraction (SFE). The antioxidant capacity was evaluated using the ferric reducing/antioxidant power (FRAP) assay and the free radical-scavenging capacity of 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The highest polyphenol content was obtained from the medium polarity methanol extract of the leaf portion (645.60 ± 166.68 gallic acid equivalents/100 g (GAEs/100 g)). It also showed the highest antioxidant power for FRAP and DPPH radical inhibition and exhibited the largest inhibition zone in antibacterial activity on Bacillus subtilis (Gram+), Staphylococcus aureus (Gram+), and Escherichia coli (Gram-). The phase behavior and aldehyde profiles were further investigated using SFE with different cosolvents. The results indicated that a 50% ethanol-water cosolvent yielded the best aldehyde profiles in the presence of decanal, undecanal, and dodecanal.

Original languageEnglish
Article number826709
JournalInternational Journal of Chemical Engineering
Volume2015
DOIs
Publication statusPublished - 2015

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Antioxidants
Assays
Supercritical fluids
Aldehydes
Gallic Acid
Scavenging
Polyphenols
Solvent extraction
Bacilli
Phase behavior
Free radicals
Escherichia coli
Free Radicals
Methanol
Ethanol
Acids
Water
2,2-diphenyl-1-picrylhydrazyl

ASJC Scopus subject areas

  • Chemical Engineering(all)

Cite this

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title = "Antioxidant and antibacterial assays on polygonum minus extracts: Different extraction methods",
abstract = "The effect of solvent type and extraction method was investigated to study the antioxidant and antibacterial activity of Polygonum minus. Two extraction methods were used: a solvent extraction using Soxhlet apparatus and supercritical fluid extraction (SFE). The antioxidant capacity was evaluated using the ferric reducing/antioxidant power (FRAP) assay and the free radical-scavenging capacity of 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The highest polyphenol content was obtained from the medium polarity methanol extract of the leaf portion (645.60 ± 166.68 gallic acid equivalents/100 g (GAEs/100 g)). It also showed the highest antioxidant power for FRAP and DPPH radical inhibition and exhibited the largest inhibition zone in antibacterial activity on Bacillus subtilis (Gram+), Staphylococcus aureus (Gram+), and Escherichia coli (Gram-). The phase behavior and aldehyde profiles were further investigated using SFE with different cosolvents. The results indicated that a 50{\%} ethanol-water cosolvent yielded the best aldehyde profiles in the presence of decanal, undecanal, and dodecanal.",
author = "Norsyamimi Hassim and Masturah Markom and Nurina Anuar and Dewi, {Kurnia Harlina} and Baharum, {Syarul Nataqain} and {Mohd. Noor}, Normah",
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T2 - Different extraction methods

AU - Hassim, Norsyamimi

AU - Markom, Masturah

AU - Anuar, Nurina

AU - Dewi, Kurnia Harlina

AU - Baharum, Syarul Nataqain

AU - Mohd. Noor, Normah

PY - 2015

Y1 - 2015

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AB - The effect of solvent type and extraction method was investigated to study the antioxidant and antibacterial activity of Polygonum minus. Two extraction methods were used: a solvent extraction using Soxhlet apparatus and supercritical fluid extraction (SFE). The antioxidant capacity was evaluated using the ferric reducing/antioxidant power (FRAP) assay and the free radical-scavenging capacity of 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The highest polyphenol content was obtained from the medium polarity methanol extract of the leaf portion (645.60 ± 166.68 gallic acid equivalents/100 g (GAEs/100 g)). It also showed the highest antioxidant power for FRAP and DPPH radical inhibition and exhibited the largest inhibition zone in antibacterial activity on Bacillus subtilis (Gram+), Staphylococcus aureus (Gram+), and Escherichia coli (Gram-). The phase behavior and aldehyde profiles were further investigated using SFE with different cosolvents. The results indicated that a 50% ethanol-water cosolvent yielded the best aldehyde profiles in the presence of decanal, undecanal, and dodecanal.

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