Antioxidant activity of red algae Kappaphycus alvarezii and Kappaphycus striatum

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Abstract

Antioxidants in seaweeds have attracted increasing interest for its role in protecting human health. Therefore, the aim of this study was to assess the Total phenolic content (TPC) values and antioxidant activities in red seaweeds Kappaphycus alvarezii and Kappaphycus striatum of different solvent extracts. Total phenolic content (TPC) and antioxidant activities (DPPH scavenging assay and Trolox equivalent antioxidant capacity assay, TEAC) for both K. alvarezii and K. striatum extracts were determined using different solvents at different concentrations (ethanol: 50%, 70%, 100%; acetone: 50%, 70%, 100%; methanol: 50%, 70%, 100%). The TPC value was measured using the Folin-Ciocalteu's method. The antioxidant activities were measured by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and Trolox Equivalent Antioxidant Capacity (TEAC) assay. The highest TPC value of K. alvarezii antioxidant extract was obtained by 50% ethanol extracts while for K. striatum obtained by 50% methanol extract. The highest percentage of DPPH free radical inhibition for K. alvarezii was shown by 50% acetone extract while K. striatum was shown using 50% methanol extract. The highest TEAC value for K. alvarezii was shown by 50% acetone while K. striatum extract was shown by 50% ethanol extract. The TPC values and antioxidant activities of all solvent extracts of K. striatum were significantly higher (p<0.05) than K. alvarezii antioxidant extracts. The TPC values showed strong correlation (r = 0.797) with TEAC values for K. alvarezii antioxidant extract (p<0.01). The TEAC values also showed strong correlation (r = 0.735) with percentage of DPPH free radical inhibition for K. alvarezii (p<0.01). The TPC value, DPPH free radical scavenging assay and TEAC assay for K. striatum extracts showed strong correlation (r > 0.8) with each other (p<0.01). In summary, K. striatum showed better antioxidant activity and higher TPC value than K. alvarezii.

Original languageEnglish
Pages (from-to)1977-1984
Number of pages8
JournalInternational Food Research Journal
Volume22
Issue number5
Publication statusPublished - 2015

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Kappaphycus
Kappaphycus alvarezii
Rhodophyta
Antioxidants
antioxidant activity
extracts
antioxidants
Acetone
acetone
methanol
Methanol
Seaweed
ethanol
Ethanol
assays
macroalgae
Free Radicals
human health

Keywords

  • DPPH free radical scavenging assay
  • Red seaweeds
  • Solvent
  • TEAC
  • TPC

ASJC Scopus subject areas

  • Food Science

Cite this

@article{cc56e0be997f40b18e2ffa78f84a0caf,
title = "Antioxidant activity of red algae Kappaphycus alvarezii and Kappaphycus striatum",
abstract = "Antioxidants in seaweeds have attracted increasing interest for its role in protecting human health. Therefore, the aim of this study was to assess the Total phenolic content (TPC) values and antioxidant activities in red seaweeds Kappaphycus alvarezii and Kappaphycus striatum of different solvent extracts. Total phenolic content (TPC) and antioxidant activities (DPPH scavenging assay and Trolox equivalent antioxidant capacity assay, TEAC) for both K. alvarezii and K. striatum extracts were determined using different solvents at different concentrations (ethanol: 50{\%}, 70{\%}, 100{\%}; acetone: 50{\%}, 70{\%}, 100{\%}; methanol: 50{\%}, 70{\%}, 100{\%}). The TPC value was measured using the Folin-Ciocalteu's method. The antioxidant activities were measured by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and Trolox Equivalent Antioxidant Capacity (TEAC) assay. The highest TPC value of K. alvarezii antioxidant extract was obtained by 50{\%} ethanol extracts while for K. striatum obtained by 50{\%} methanol extract. The highest percentage of DPPH free radical inhibition for K. alvarezii was shown by 50{\%} acetone extract while K. striatum was shown using 50{\%} methanol extract. The highest TEAC value for K. alvarezii was shown by 50{\%} acetone while K. striatum extract was shown by 50{\%} ethanol extract. The TPC values and antioxidant activities of all solvent extracts of K. striatum were significantly higher (p<0.05) than K. alvarezii antioxidant extracts. The TPC values showed strong correlation (r = 0.797) with TEAC values for K. alvarezii antioxidant extract (p<0.01). The TEAC values also showed strong correlation (r = 0.735) with percentage of DPPH free radical inhibition for K. alvarezii (p<0.01). The TPC value, DPPH free radical scavenging assay and TEAC assay for K. striatum extracts showed strong correlation (r > 0.8) with each other (p<0.01). In summary, K. striatum showed better antioxidant activity and higher TPC value than K. alvarezii.",
keywords = "DPPH free radical scavenging assay, Red seaweeds, Solvent, TEAC, TPC",
author = "{Farah Diyana}, A. and Aminah Abdullah and {Zainal Ariffin}, {Shahrul Hisham} and {Kok Meng}, Chan",
year = "2015",
language = "English",
volume = "22",
pages = "1977--1984",
journal = "International Food Research Journal",
issn = "1985-4668",
publisher = "Universiti Putra Malaysia",
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T1 - Antioxidant activity of red algae Kappaphycus alvarezii and Kappaphycus striatum

AU - Farah Diyana, A.

AU - Abdullah, Aminah

AU - Zainal Ariffin, Shahrul Hisham

AU - Kok Meng, Chan

PY - 2015

Y1 - 2015

N2 - Antioxidants in seaweeds have attracted increasing interest for its role in protecting human health. Therefore, the aim of this study was to assess the Total phenolic content (TPC) values and antioxidant activities in red seaweeds Kappaphycus alvarezii and Kappaphycus striatum of different solvent extracts. Total phenolic content (TPC) and antioxidant activities (DPPH scavenging assay and Trolox equivalent antioxidant capacity assay, TEAC) for both K. alvarezii and K. striatum extracts were determined using different solvents at different concentrations (ethanol: 50%, 70%, 100%; acetone: 50%, 70%, 100%; methanol: 50%, 70%, 100%). The TPC value was measured using the Folin-Ciocalteu's method. The antioxidant activities were measured by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and Trolox Equivalent Antioxidant Capacity (TEAC) assay. The highest TPC value of K. alvarezii antioxidant extract was obtained by 50% ethanol extracts while for K. striatum obtained by 50% methanol extract. The highest percentage of DPPH free radical inhibition for K. alvarezii was shown by 50% acetone extract while K. striatum was shown using 50% methanol extract. The highest TEAC value for K. alvarezii was shown by 50% acetone while K. striatum extract was shown by 50% ethanol extract. The TPC values and antioxidant activities of all solvent extracts of K. striatum were significantly higher (p<0.05) than K. alvarezii antioxidant extracts. The TPC values showed strong correlation (r = 0.797) with TEAC values for K. alvarezii antioxidant extract (p<0.01). The TEAC values also showed strong correlation (r = 0.735) with percentage of DPPH free radical inhibition for K. alvarezii (p<0.01). The TPC value, DPPH free radical scavenging assay and TEAC assay for K. striatum extracts showed strong correlation (r > 0.8) with each other (p<0.01). In summary, K. striatum showed better antioxidant activity and higher TPC value than K. alvarezii.

AB - Antioxidants in seaweeds have attracted increasing interest for its role in protecting human health. Therefore, the aim of this study was to assess the Total phenolic content (TPC) values and antioxidant activities in red seaweeds Kappaphycus alvarezii and Kappaphycus striatum of different solvent extracts. Total phenolic content (TPC) and antioxidant activities (DPPH scavenging assay and Trolox equivalent antioxidant capacity assay, TEAC) for both K. alvarezii and K. striatum extracts were determined using different solvents at different concentrations (ethanol: 50%, 70%, 100%; acetone: 50%, 70%, 100%; methanol: 50%, 70%, 100%). The TPC value was measured using the Folin-Ciocalteu's method. The antioxidant activities were measured by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay and Trolox Equivalent Antioxidant Capacity (TEAC) assay. The highest TPC value of K. alvarezii antioxidant extract was obtained by 50% ethanol extracts while for K. striatum obtained by 50% methanol extract. The highest percentage of DPPH free radical inhibition for K. alvarezii was shown by 50% acetone extract while K. striatum was shown using 50% methanol extract. The highest TEAC value for K. alvarezii was shown by 50% acetone while K. striatum extract was shown by 50% ethanol extract. The TPC values and antioxidant activities of all solvent extracts of K. striatum were significantly higher (p<0.05) than K. alvarezii antioxidant extracts. The TPC values showed strong correlation (r = 0.797) with TEAC values for K. alvarezii antioxidant extract (p<0.01). The TEAC values also showed strong correlation (r = 0.735) with percentage of DPPH free radical inhibition for K. alvarezii (p<0.01). The TPC value, DPPH free radical scavenging assay and TEAC assay for K. striatum extracts showed strong correlation (r > 0.8) with each other (p<0.01). In summary, K. striatum showed better antioxidant activity and higher TPC value than K. alvarezii.

KW - DPPH free radical scavenging assay

KW - Red seaweeds

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KW - TEAC

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M3 - Article

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