Antimycobacterial activity of selected medicinal plants traditionally used in Sudan to treat infectious diseases

Nadir Abuzeid, Sadaf Kalsum, Marie Larsson, Mikaela Glader, Henrik Andersson, Johanna Raffetseder, Elsje Pienaar, Daniel Eklund, Muddathir S. Alhassan, Haidar A. Algadir, Waleed S. Koko, Thomas Schön, Osman Mesaik Mohammed Ahmed Hassan, Omer M. Abdalla, Asaad Khalid, Maria Lerm

Research output: Contribution to journalArticle

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Abstract

Ethnopharmacological relevance: The emergence of multidrug-resistant strains of Mycobacterium tuberculosis underscores the need for continuous development of new and efficient methods to determine the susceptibility of isolates of Mycobacterium tuberculosis in the search for novel antimycobacterial agents. Natural products constitute an important source of new drugs, and design and implementation of antimycobacterial susceptibility testing methods are necessary to evaluate the different extracts and compounds. In this study we have explored the antimycobacterial properties of 50 ethanolic extracts from different parts of 46 selected medicinal plants traditionally used in Sudan to treat infectious diseases.

Materials and methods: Plants were harvested and ethanolic extracts were prepared. For selected extracts, fractionation with hydrophilic and hydrophobic solvents was undertaken. A luminometrybased assay was used for determination of mycobacterial growth in broth cultures and inside primary human macrophages in the presence or absence of plant extracts and fractions of extracts. Cytotoxicity was also assessed for active fractions of plant extracts.

Results: Of the tested extracts, three exhibited a significant inhibitory effect on an avirulent strain of Mycobacterium tube rcluosis (1-I37Ra) at the initial screening doses (125 and 625 igml). These were bark and leaf extracts of Khaya senegalensis and the leaf extract of Rosmarinus offlcinalisL. Further fractions of these plant extracts were prepared with n-hexane, chloroform, ethyl acetate, n-butanol, ethanol and water, and the activity of these extracts was retained in hydrophobic fractions. Cytotoxicity assays revealed that the chloroform fraction of Khaya senegalensis bark was non-toxic to human monocytederived macrophages and other cell types at the concentrations used and hence, further analysis, including assessment of 1C50 and intracellular activity was done with this fraction.

Conclusion: These results encourage further investigations to identify the active compound(s) within the chloroform fraction of Khaya senegalensis bark.

Original languageEnglish
Pages (from-to)134-139
Number of pages6
JournalJournal of Ethnopharmacology
Volume157
DOIs
Publication statusPublished - 18 Nov 2014

Fingerprint

Meliaceae
Sudan
Plant Extracts
Medicinal Plants
Chloroform
Communicable Diseases
Mycobacterium tuberculosis
Rosmarinus
Macrophages
1-Butanol
Drug Design
Mycobacterium
Biological Products
Ethanol
Anti-Bacterial Agents
Water
Growth

Keywords

  • Cytotoxicity assay
  • Luminescence reporter assay
  • Mycobacterium tuberculosis
  • Primary human macrophages
  • Sudanese medicinal plants

ASJC Scopus subject areas

  • Pharmacology
  • Drug Discovery
  • Medicine(all)

Cite this

Abuzeid, N., Kalsum, S., Larsson, M., Glader, M., Andersson, H., Raffetseder, J., ... Lerm, M. (2014). Antimycobacterial activity of selected medicinal plants traditionally used in Sudan to treat infectious diseases. Journal of Ethnopharmacology, 157, 134-139. https://doi.org/10.1016/j.jep.2014.09.020

Antimycobacterial activity of selected medicinal plants traditionally used in Sudan to treat infectious diseases. / Abuzeid, Nadir; Kalsum, Sadaf; Larsson, Marie; Glader, Mikaela; Andersson, Henrik; Raffetseder, Johanna; Pienaar, Elsje; Eklund, Daniel; Alhassan, Muddathir S.; Algadir, Haidar A.; Koko, Waleed S.; Schön, Thomas; Mohammed Ahmed Hassan, Osman Mesaik; Abdalla, Omer M.; Khalid, Asaad; Lerm, Maria.

In: Journal of Ethnopharmacology, Vol. 157, 18.11.2014, p. 134-139.

Research output: Contribution to journalArticle

Abuzeid, N, Kalsum, S, Larsson, M, Glader, M, Andersson, H, Raffetseder, J, Pienaar, E, Eklund, D, Alhassan, MS, Algadir, HA, Koko, WS, Schön, T, Mohammed Ahmed Hassan, OM, Abdalla, OM, Khalid, A & Lerm, M 2014, 'Antimycobacterial activity of selected medicinal plants traditionally used in Sudan to treat infectious diseases', Journal of Ethnopharmacology, vol. 157, pp. 134-139. https://doi.org/10.1016/j.jep.2014.09.020
Abuzeid, Nadir ; Kalsum, Sadaf ; Larsson, Marie ; Glader, Mikaela ; Andersson, Henrik ; Raffetseder, Johanna ; Pienaar, Elsje ; Eklund, Daniel ; Alhassan, Muddathir S. ; Algadir, Haidar A. ; Koko, Waleed S. ; Schön, Thomas ; Mohammed Ahmed Hassan, Osman Mesaik ; Abdalla, Omer M. ; Khalid, Asaad ; Lerm, Maria. / Antimycobacterial activity of selected medicinal plants traditionally used in Sudan to treat infectious diseases. In: Journal of Ethnopharmacology. 2014 ; Vol. 157. pp. 134-139.
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