Antimicrobial resistance profiling and molecular typing of methicillin-resistant Staphylococcus aureus isolated from a Malaysian teaching hospital

Ainihayati Noordin, Hassriana Fazzila Sapri, Nurul Azirah Mohamad Sani, Shih Kei Leong, Xin Ee Tan, Toh Leong Tan, Noraziah Mohamad Zin, Hui Min Neoh, Salasawati Hussin

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The annual prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in Malaysia has been estimated to be 30% to 40% of all S. aureus infections. Nevertheless, data on the antimicrobial resistance and genetic diversity of Malaysian MRSAs remain few. In 2009, we collected 318 MRSA strains from various wards of our teaching hospital located in Kuala Lumpur, the capital city of Malaysia, and performed antimicrobial susceptibility testing on these strains. The strains were then molecularly characterized via staphylococcal cassette chromosome (SCC) mec and virulence gene (cna, sea, seb, sec, sed, see, seg, seh, sei, eta, etb, Panton-Valentine leukocidin and toxic shock syndrome toxin-1) typing; a subset of 49 strains isolated from the intensive care unit was also typed using PFGE. Most strains were found to be resistant to ciprofloxacin (92.5%), erythromycin (93.4%) and gentamicin (86.8%). The majority (72.0%) of strains were found to harbour SCCmec type III-SCCmercurywith the presence of ccrC, and carried the sea+cna gene combination (49.3%), with cna as the most prevalent virulence gene (94.0%) detected. We identified four PFGE clusters, with pulsotype C (n=19) as the dominant example in the intensive care unit, where this pulsotype was found to be associated with carriage of SCCmec type III and the sea gene (P=0.05 and P=0.02, respectively). In summary, the dominant MRSA circulating in our hospital in 2009 was a clone that was ciprofloxacin, erythromycin and gentamicin resistant, carried SCCmec type III-SCCmercury with ccrC and also harboured the sea+cna virulence genes. This clone also appears to be the dominant MRSA circulating in major hospitals in Kuala Lumpur.

Original languageEnglish
Article number000387
Pages (from-to)1476-1481
Number of pages6
JournalJournal of Medical Microbiology
Volume65
Issue number12
DOIs
Publication statusPublished - 1 Dec 2016

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Molecular Typing
Methicillin-Resistant Staphylococcus aureus
Teaching Hospitals
Oceans and Seas
Virulence
Genes
Malaysia
Erythromycin
Ciprofloxacin
Gentamicins
Intensive Care Units
Clone Cells
Staphylococcus aureus
Chromosomes
Infection

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Antimicrobial resistance profiling and molecular typing of methicillin-resistant Staphylococcus aureus isolated from a Malaysian teaching hospital. / Noordin, Ainihayati; Sapri, Hassriana Fazzila; Sani, Nurul Azirah Mohamad; Leong, Shih Kei; Tan, Xin Ee; Tan, Toh Leong; Mohamad Zin, Noraziah; Neoh, Hui Min; Hussin, Salasawati.

In: Journal of Medical Microbiology, Vol. 65, No. 12, 000387, 01.12.2016, p. 1476-1481.

Research output: Contribution to journalArticle

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AU - Leong, Shih Kei

AU - Tan, Xin Ee

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