Antimicrobial property of water and ethanol extract Chlorella vulgaris

A value-added advantage for a new wound dressing material

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Objective: Chlorella vulgaris (CV) is a unicellular green microalgae enriched with nutrients, vitamins and minerals. It has been reported to have better wound healing effect in comparison with calcium sodium alginate (brown algae) dressing. However, calcium sodium alginate dressing does not have antimicrobial property. The aim of our study was to determine the antimicrobial property of CV towards three gram-positive bacteria: Staphylococcus aureus ATCC 25923, Streptococcus pyogenes ATCC 12344, Enterococcus faecalis ATCC 29212, and two gram-negative bacteria: Pseudomonas aeruginosa ATCC 29212 and Escherichia coli ATCC 11230. Methods: The minimum inhibitory concentration (MIC) of water and ethanol extracts of CV was determined by agar-well diffusion method in different concentrations (100 mg/mL, 300 mg/mL and 500 mg/mL). Gentamicin disc was used for Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Pseudomonas aeruginosa ATCC 29212 and Escherichia coli ATCC 11230. Erythromycin disc was used for Streptococcus pyogenes ATCC12344 as positive control for inhibitory activity. Results: Ethanol extract of CV (all concentrations) showed inhibitory activity against all tested bacteria with mean diameter of inhibition zones between 6-14 ± 0.58 mm. The aqueous extracts of CV only showed minimal activity towards Escherichia coli ATCC 11230 with mean zone of inhibition of 7 ± 1.16 mm regardless of the concentrations used. On the other hand, the MIC values determined by broth macrodilution were between 125 μg/mL to 1000 μg/mL. Conclusion: CV has an antimicrobial activity in addition to its wound healing ability. CV has the potential to be developed as an alternative wound dressing material.

Original languageEnglish
Pages (from-to)399-401
Number of pages3
JournalInternational Medical Journal
Volume22
Issue number5
Publication statusPublished - 2015

Fingerprint

Chlorella vulgaris
Bandages
Ethanol
Water
Wounds and Injuries
Enterococcus faecalis
Streptococcus pyogenes
Microbial Sensitivity Tests
Escherichia coli
Wound Healing
Pseudomonas aeruginosa
Staphylococcus aureus
Phaeophyta
Microalgae
Gram-Positive Bacteria
Erythromycin
Gentamicins
Gram-Negative Bacteria
Vitamins
Agar

Keywords

  • Agar well diffusion method
  • Antimicrobial
  • Chlorella vulgaris
  • Minimum inhibitory concentration
  • Wound dressing

ASJC Scopus subject areas

  • Medicine(all)

Cite this

@article{b534fbebc548441c89345cbfca75bc02,
title = "Antimicrobial property of water and ethanol extract Chlorella vulgaris: A value-added advantage for a new wound dressing material",
abstract = "Objective: Chlorella vulgaris (CV) is a unicellular green microalgae enriched with nutrients, vitamins and minerals. It has been reported to have better wound healing effect in comparison with calcium sodium alginate (brown algae) dressing. However, calcium sodium alginate dressing does not have antimicrobial property. The aim of our study was to determine the antimicrobial property of CV towards three gram-positive bacteria: Staphylococcus aureus ATCC 25923, Streptococcus pyogenes ATCC 12344, Enterococcus faecalis ATCC 29212, and two gram-negative bacteria: Pseudomonas aeruginosa ATCC 29212 and Escherichia coli ATCC 11230. Methods: The minimum inhibitory concentration (MIC) of water and ethanol extracts of CV was determined by agar-well diffusion method in different concentrations (100 mg/mL, 300 mg/mL and 500 mg/mL). Gentamicin disc was used for Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Pseudomonas aeruginosa ATCC 29212 and Escherichia coli ATCC 11230. Erythromycin disc was used for Streptococcus pyogenes ATCC12344 as positive control for inhibitory activity. Results: Ethanol extract of CV (all concentrations) showed inhibitory activity against all tested bacteria with mean diameter of inhibition zones between 6-14 ± 0.58 mm. The aqueous extracts of CV only showed minimal activity towards Escherichia coli ATCC 11230 with mean zone of inhibition of 7 ± 1.16 mm regardless of the concentrations used. On the other hand, the MIC values determined by broth macrodilution were between 125 μg/mL to 1000 μg/mL. Conclusion: CV has an antimicrobial activity in addition to its wound healing ability. CV has the potential to be developed as an alternative wound dressing material.",
keywords = "Agar well diffusion method, Antimicrobial, Chlorella vulgaris, Minimum inhibitory concentration, Wound dressing",
author = "Kamalnizat Ibrahim and Ramliza Ramli and {Abd Rashid}, {Abdul Halim} and {Mohd Yusof}, {Yasmin Anum}",
year = "2015",
language = "English",
volume = "22",
pages = "399--401",
journal = "International Medical Journal",
issn = "1341-2051",
publisher = "Japan International Cultural Exchange Foundation",
number = "5",

}

TY - JOUR

T1 - Antimicrobial property of water and ethanol extract Chlorella vulgaris

T2 - A value-added advantage for a new wound dressing material

AU - Ibrahim, Kamalnizat

AU - Ramli, Ramliza

AU - Abd Rashid, Abdul Halim

AU - Mohd Yusof, Yasmin Anum

PY - 2015

Y1 - 2015

N2 - Objective: Chlorella vulgaris (CV) is a unicellular green microalgae enriched with nutrients, vitamins and minerals. It has been reported to have better wound healing effect in comparison with calcium sodium alginate (brown algae) dressing. However, calcium sodium alginate dressing does not have antimicrobial property. The aim of our study was to determine the antimicrobial property of CV towards three gram-positive bacteria: Staphylococcus aureus ATCC 25923, Streptococcus pyogenes ATCC 12344, Enterococcus faecalis ATCC 29212, and two gram-negative bacteria: Pseudomonas aeruginosa ATCC 29212 and Escherichia coli ATCC 11230. Methods: The minimum inhibitory concentration (MIC) of water and ethanol extracts of CV was determined by agar-well diffusion method in different concentrations (100 mg/mL, 300 mg/mL and 500 mg/mL). Gentamicin disc was used for Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Pseudomonas aeruginosa ATCC 29212 and Escherichia coli ATCC 11230. Erythromycin disc was used for Streptococcus pyogenes ATCC12344 as positive control for inhibitory activity. Results: Ethanol extract of CV (all concentrations) showed inhibitory activity against all tested bacteria with mean diameter of inhibition zones between 6-14 ± 0.58 mm. The aqueous extracts of CV only showed minimal activity towards Escherichia coli ATCC 11230 with mean zone of inhibition of 7 ± 1.16 mm regardless of the concentrations used. On the other hand, the MIC values determined by broth macrodilution were between 125 μg/mL to 1000 μg/mL. Conclusion: CV has an antimicrobial activity in addition to its wound healing ability. CV has the potential to be developed as an alternative wound dressing material.

AB - Objective: Chlorella vulgaris (CV) is a unicellular green microalgae enriched with nutrients, vitamins and minerals. It has been reported to have better wound healing effect in comparison with calcium sodium alginate (brown algae) dressing. However, calcium sodium alginate dressing does not have antimicrobial property. The aim of our study was to determine the antimicrobial property of CV towards three gram-positive bacteria: Staphylococcus aureus ATCC 25923, Streptococcus pyogenes ATCC 12344, Enterococcus faecalis ATCC 29212, and two gram-negative bacteria: Pseudomonas aeruginosa ATCC 29212 and Escherichia coli ATCC 11230. Methods: The minimum inhibitory concentration (MIC) of water and ethanol extracts of CV was determined by agar-well diffusion method in different concentrations (100 mg/mL, 300 mg/mL and 500 mg/mL). Gentamicin disc was used for Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Pseudomonas aeruginosa ATCC 29212 and Escherichia coli ATCC 11230. Erythromycin disc was used for Streptococcus pyogenes ATCC12344 as positive control for inhibitory activity. Results: Ethanol extract of CV (all concentrations) showed inhibitory activity against all tested bacteria with mean diameter of inhibition zones between 6-14 ± 0.58 mm. The aqueous extracts of CV only showed minimal activity towards Escherichia coli ATCC 11230 with mean zone of inhibition of 7 ± 1.16 mm regardless of the concentrations used. On the other hand, the MIC values determined by broth macrodilution were between 125 μg/mL to 1000 μg/mL. Conclusion: CV has an antimicrobial activity in addition to its wound healing ability. CV has the potential to be developed as an alternative wound dressing material.

KW - Agar well diffusion method

KW - Antimicrobial

KW - Chlorella vulgaris

KW - Minimum inhibitory concentration

KW - Wound dressing

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