Antimicrobial properties of the acetone leaves and stems extracts of Clinacanthus nutans from three different samples/areas against pathogenic microorganisms

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Abstract

Clinacanthus nutans is the native medicinal herbs that grow in tropical climate, mainly can be found in Malaysia and Thailand. Clinacanthus nutans is used to boost immunity and for detoxification. It is claiming to promote bowel movement and other health promotions, such as skin care, diuresis and cancer (such as lung, ovary, uterine, prostate, nasopharyngeal and breast). This study focused on the effects of solvents towards antioxidant and antimicrobial properties of C. nutans (Belalai Gajah/ Sabah Snake Grass) leaves and stems. Freeze-dried leaves and stems were homogenized before testing its antimicrobial properties. The 70% acetone (Merck, Germany) was prepared by dissolving fine powder of C. nutans in 1:10 solvent ratio with 70% acetone using ultrasonic extraction for 1 h in an ultrasonic bath (Soniclean, Thebarton, Australia). Ten bacteria (Bacillus cereus, B. subtilis, Escherichia coli, Klebsiella pneumonia, Listeria monocytogenes, Pseudomonas aureginosa, Salmonella typhimurium, Shigella boydii, Staphylococcus aureus and S. epidemidis) and four fungi (Aspergillus niger, Candida albicans, Rhizopus stolonifer and Saccharomyces cerevisiae) were used in the antimicrobial study employing minimum inhibitory concentration (MIC) and minimal microcidal concentration (MMC) methods. The data obtained were statistically analysed using SPSS Inc. Version 22 (Chicago, USA). The MIC values of acetone extracts for the selected bacteria and fungi ranged between 6.25 - 100.00 mg/ mL while the MMC values of acetone extracts for the selected bacteria and fungi were between 25.00 - > 100.00 mg/ mL. Recommendation on phytochemicals investigation should be conducted to determine the potential of compounds toward antioxidant and antimicrobial properties.

Original languageEnglish
Pages (from-to)1698-1702
Number of pages5
JournalInternational Food Research Journal
Volume25
Issue number4
Publication statusPublished - 1 Jan 2018

Fingerprint

anti-infective properties
Acetone
acetone
microorganisms
stems
Fungi
Malaysia
extracts
Microbial Sensitivity Tests
minimum inhibitory concentration
Bacteria
Ultrasonics
fungi
leaves
ultrasonics
bacteria
Shigella boydii
Antioxidants
Tropical Climate
Skin Care

Keywords

  • And Oceania
  • Antifungal
  • Antimicrobial
  • Botany
  • Traditional medicine Asia

ASJC Scopus subject areas

  • Food Science

Cite this

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title = "Antimicrobial properties of the acetone leaves and stems extracts of Clinacanthus nutans from three different samples/areas against pathogenic microorganisms",
abstract = "Clinacanthus nutans is the native medicinal herbs that grow in tropical climate, mainly can be found in Malaysia and Thailand. Clinacanthus nutans is used to boost immunity and for detoxification. It is claiming to promote bowel movement and other health promotions, such as skin care, diuresis and cancer (such as lung, ovary, uterine, prostate, nasopharyngeal and breast). This study focused on the effects of solvents towards antioxidant and antimicrobial properties of C. nutans (Belalai Gajah/ Sabah Snake Grass) leaves and stems. Freeze-dried leaves and stems were homogenized before testing its antimicrobial properties. The 70{\%} acetone (Merck, Germany) was prepared by dissolving fine powder of C. nutans in 1:10 solvent ratio with 70{\%} acetone using ultrasonic extraction for 1 h in an ultrasonic bath (Soniclean, Thebarton, Australia). Ten bacteria (Bacillus cereus, B. subtilis, Escherichia coli, Klebsiella pneumonia, Listeria monocytogenes, Pseudomonas aureginosa, Salmonella typhimurium, Shigella boydii, Staphylococcus aureus and S. epidemidis) and four fungi (Aspergillus niger, Candida albicans, Rhizopus stolonifer and Saccharomyces cerevisiae) were used in the antimicrobial study employing minimum inhibitory concentration (MIC) and minimal microcidal concentration (MMC) methods. The data obtained were statistically analysed using SPSS Inc. Version 22 (Chicago, USA). The MIC values of acetone extracts for the selected bacteria and fungi ranged between 6.25 - 100.00 mg/ mL while the MMC values of acetone extracts for the selected bacteria and fungi were between 25.00 - > 100.00 mg/ mL. Recommendation on phytochemicals investigation should be conducted to determine the potential of compounds toward antioxidant and antimicrobial properties.",
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AU - Kong, H. S.

AU - Abdullah Sani, Norrakiah

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AB - Clinacanthus nutans is the native medicinal herbs that grow in tropical climate, mainly can be found in Malaysia and Thailand. Clinacanthus nutans is used to boost immunity and for detoxification. It is claiming to promote bowel movement and other health promotions, such as skin care, diuresis and cancer (such as lung, ovary, uterine, prostate, nasopharyngeal and breast). This study focused on the effects of solvents towards antioxidant and antimicrobial properties of C. nutans (Belalai Gajah/ Sabah Snake Grass) leaves and stems. Freeze-dried leaves and stems were homogenized before testing its antimicrobial properties. The 70% acetone (Merck, Germany) was prepared by dissolving fine powder of C. nutans in 1:10 solvent ratio with 70% acetone using ultrasonic extraction for 1 h in an ultrasonic bath (Soniclean, Thebarton, Australia). Ten bacteria (Bacillus cereus, B. subtilis, Escherichia coli, Klebsiella pneumonia, Listeria monocytogenes, Pseudomonas aureginosa, Salmonella typhimurium, Shigella boydii, Staphylococcus aureus and S. epidemidis) and four fungi (Aspergillus niger, Candida albicans, Rhizopus stolonifer and Saccharomyces cerevisiae) were used in the antimicrobial study employing minimum inhibitory concentration (MIC) and minimal microcidal concentration (MMC) methods. The data obtained were statistically analysed using SPSS Inc. Version 22 (Chicago, USA). The MIC values of acetone extracts for the selected bacteria and fungi ranged between 6.25 - 100.00 mg/ mL while the MMC values of acetone extracts for the selected bacteria and fungi were between 25.00 - > 100.00 mg/ mL. Recommendation on phytochemicals investigation should be conducted to determine the potential of compounds toward antioxidant and antimicrobial properties.

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