Antimicrobial efficacy against acanthamoeba isolates from clinical cases

Mohamed Kamel Abdul Ghani, S. R. Hany Sady, Ahmad Zorin Sahalan, Hiang Lian Hing, Anisah Nordin, Yusof Suboh, Noraina Ab Rahim, Norazah Ahmad

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Introduction: Acanthamoeba spp. are free-living protozoan parasites that can cause painful, severe ocular inflammation and sight-threatening keratitis. Acanthamoeba keratitis is a widespread infectious corneal disease that has been reported worldwide. Objective: This study investigates the effectiveness and the range of minimum cysticidal concentration (MCC) of four antimicrobial agents in therapeutic doses; 0.02% chlorhexidine, 0.1% propamidine isethionate (Brolene®), 0.3% ciprofloxacin and 0.3% gentamicin against 4 clinical isolates of Acanthamoeba. Four isolates of Acanthamoeba cysts being used were derived from clinical cases (HKL 95, HKL 109, HS 5 and HS 6). Materials and methods: Serial doubling dilutions of the antimicrobial agents were performed in microtiter plates. After exposure of the four Acanthamoeba isolate cysts to the four antimicrobial agents for 24 hours, the cysts were washed three times with PAS and centrifuged. The deposits (cysts) were cultured onto non-nutrient agar coated with heat-killed Escherichia coli. The excystment of trophozoites from cysts was observed and recorded microscopically for 14 days to determine the MCC value of each drug. Results: Results of this study showed that chlorhexidine, propamidine isethionate, ciprofloxacin and gentamicin successfully exhibited their cysticidal activities in therapeutic doses on all isolates. The values of MCC for chlorhexidine, propamidine isethionate, ciprofloxacin and gentamicin varied from 6.25 to 25 μg/ml, 125 to 250 μg/ml, 1500 to 3000 μg/ml and 1500 to 3000 μg/ml, respectively. Moreover, chlorhexidine, propamidine isethionate, ciprofloxacin and gentamicin were found to exhibit cysticidal activities with their mean MCC values of 17.2 ± 9.4 μg/ml, 156.3 ± 62.5 μg/ml, 2625 ± 750 μg/ml and 1875 ± 750 μg/ml, respectively. Conclusion: The in vitro sensitivity test can be used as a standard test to determine values of MCC of drugs on Acanthamoeba isolates and to study the susceptibility pattern of anti-Acanthamoeba drugs. Chlorhexidine and Propamidine isethionate exhibited the best anti-Acanthamoeba activity and are therefore highly recommended for use in the treatment of Acanthamoeba keratitis.

Original languageEnglish
Pages (from-to)112-115
Number of pages4
JournalInternational Medical Journal
Volume25
Issue number2
Publication statusPublished - 1 Apr 2018

Fingerprint

Acanthamoeba
Chlorhexidine
Cysts
Ciprofloxacin
Gentamicins
Anti-Infective Agents
Acanthamoeba Keratitis
HS 6
Pharmaceutical Preparations
Corneal Diseases
Trophozoites
Keratitis
Agar
Communicable Diseases
Parasites
Hot Temperature
propamidine isethionate
Escherichia coli
Inflammation
Therapeutics

Keywords

  • Acanthamoeba
  • Antimicrobial
  • Keratitis
  • Malaysia
  • Minimum cysticidal concentration

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Antimicrobial efficacy against acanthamoeba isolates from clinical cases. / Abdul Ghani, Mohamed Kamel; Hany Sady, S. R.; Sahalan, Ahmad Zorin; Hing, Hiang Lian; Nordin, Anisah; Suboh, Yusof; Rahim, Noraina Ab; Ahmad, Norazah.

In: International Medical Journal, Vol. 25, No. 2, 01.04.2018, p. 112-115.

Research output: Contribution to journalArticle

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AU - Hany Sady, S. R.

AU - Sahalan, Ahmad Zorin

AU - Hing, Hiang Lian

AU - Nordin, Anisah

AU - Suboh, Yusof

AU - Rahim, Noraina Ab

AU - Ahmad, Norazah

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