Antimicrobial and cytotoxic properties of some Malaysian traditional vegetables (ULAM)

Muhammad Mukram Mohamed Mackeen, A. M. Ali, S. H. El-Sharkawy, M. Y. Manap, K. M. Salleh, N. H. Lajis, K. Kawazu

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Ethanol extracts of 19 Malaysian traditional vegetables (locally known as 'ulam') belonging to 15 families were screened for antimicrobial and cytotoxic activities. Both the disc diffusion (qualitative) and tube dilution (quantitative) assays were employed for the determination of antimicrobial activity against six pathogenic microorganisms, i.e. two Gram-positive bacteria (Bacillus cereus and Bacillus megaterium), two Gram-negative bacteria (Escherichia coli and Pseudomonas aeruginosa) and two fungi (Aspergillus ochraceous and Cryptococcus neoformans). Six extracts, i.e. Anacardium occidentale, Garcinia atroviridis, Averrhoa bilimbi, Polygonum minus, Diplazium esculentum and Etlingera elatior, showed antimicrobial with minimum inhibitory concentration (MIC) and minimum lethal concentration (MLC) values in the respective ranges of 100 → 800 μg/ml and 400 → 800 μg/ml. Seven extracts, i.e. Anacardium occidentale, Garcinia atroviridis, Sesbania grandiflora, Barringtonia racemosa, Polygonum minus, Kaempferia galanga and Etlingera elatior displayed cytotoxic activity against the HeLa (human cervical carcinoma) cell line with CD50 values in the range of 10-30 μg/ml. The 'ulam' show potential as 'functional food' in view of the significant therapeutic and nutritive benefits.

Original languageEnglish
Pages (from-to)174-178
Number of pages5
JournalPharmaceutical Biology
Volume35
Issue number3
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Garcinia
Anacardium
Polygonum
Vegetables
Etlingera elatior
Barringtonia
Anacardium occidentale
Bacteria
Sesbania
anti-infective agents
Alpinia
vegetables
Zingiberaceae
Bacillus cereus
Bacillus megaterium
Functional Food
Cryptococcus neoformans
Barringtonia racemosa
Aspergillus
extracts

Keywords

  • 'Ulam'
  • Antimicrobial activity
  • Cytotoxic activity
  • Ethanol extracts
  • Functional food
  • Malaysian traditional vegetables

ASJC Scopus subject areas

  • Plant Science
  • Clinical Biochemistry
  • Pharmacology
  • Medical Laboratory Technology

Cite this

Mohamed Mackeen, M. M., Ali, A. M., El-Sharkawy, S. H., Manap, M. Y., Salleh, K. M., Lajis, N. H., & Kawazu, K. (1997). Antimicrobial and cytotoxic properties of some Malaysian traditional vegetables (ULAM). Pharmaceutical Biology, 35(3), 174-178.

Antimicrobial and cytotoxic properties of some Malaysian traditional vegetables (ULAM). / Mohamed Mackeen, Muhammad Mukram; Ali, A. M.; El-Sharkawy, S. H.; Manap, M. Y.; Salleh, K. M.; Lajis, N. H.; Kawazu, K.

In: Pharmaceutical Biology, Vol. 35, No. 3, 1997, p. 174-178.

Research output: Contribution to journalArticle

Mohamed Mackeen, MM, Ali, AM, El-Sharkawy, SH, Manap, MY, Salleh, KM, Lajis, NH & Kawazu, K 1997, 'Antimicrobial and cytotoxic properties of some Malaysian traditional vegetables (ULAM)', Pharmaceutical Biology, vol. 35, no. 3, pp. 174-178.
Mohamed Mackeen MM, Ali AM, El-Sharkawy SH, Manap MY, Salleh KM, Lajis NH et al. Antimicrobial and cytotoxic properties of some Malaysian traditional vegetables (ULAM). Pharmaceutical Biology. 1997;35(3):174-178.
Mohamed Mackeen, Muhammad Mukram ; Ali, A. M. ; El-Sharkawy, S. H. ; Manap, M. Y. ; Salleh, K. M. ; Lajis, N. H. ; Kawazu, K. / Antimicrobial and cytotoxic properties of some Malaysian traditional vegetables (ULAM). In: Pharmaceutical Biology. 1997 ; Vol. 35, No. 3. pp. 174-178.
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