Antihypertensive effect of piper sarmentosum in L-NAME-induced hypertensive rats

Nik Aloesnisa Nik Mohd Alwi, Zaiton Zakaria, Aminuddin Abdul Hamid Karim, Nor Anita Megat Mohd Nordin, Azizah Ugusman

Research output: Contribution to journalArticle

Abstract

Hypertension is one of the risk factors for cardiovascular diseases and has been associated with about 13% of global deaths worldwide. Oxidative stress and reduced nitric oxide (NO) bioavailability contribute to the development of endothelial dysfunction and subsequently hypertension. N-nitro-L-arginine methyl ester hydrochloride (L-NAME) inhibits NO synthesis; leading to hypertension. Piper sarmentosum (PS) is an herb with antioxidant, antiatherosclerosis and antiinflammation properties. PS also stimulated NO production by endothelial cells. The aim of this study was to determine the effects of aqueous extract of Piper sarmentosum (AEPS) on blood pressure, oxidative stress and the level of nitric oxide in L-NAME-induced hypertensive rats. Hypertension was induced by oral administration of L-NAME (100 mg/L) in drinking water for four weeks. The rats were concurrently treated with AEPS by oral gavage in serial doses (125, 250 and 500 mg/kg/day). Blood pressure was measured using non-invasive tail-cuff method at baseline and fortnightly thereafter. Serum level of NO and an oxidative stress marker, malondialdehyde (MDA) were measured at baseline and at the end of treatment. The results showed that treatment with three different doses of AEPS successfully reduced systolic blood pressure (p<0.001), diastolic blood pressure (p<0.05) and mean arterial pressure (p<0.05) in L-NAME-induced hypertensive rats. Treatment with AEPS also reduced MDA level (p<0.001) and increased serum NO (p<0.001) in L-NAME-induced hypertensive rats. The findings showed that AEPS decreased blood pressure by protecting against oxidative stress and increasing NO in L-NAME-induced hypertensive rats.

Original languageEnglish
Pages (from-to)2421-2428
Number of pages8
JournalSains Malaysiana
Volume47
Issue number10
DOIs
Publication statusPublished - 1 Oct 2018

Fingerprint

Piper
NG-Nitroarginine Methyl Ester
Antihypertensive Agents
Nitric Oxide
Blood Pressure
Oxidative Stress
Hypertension
Malondialdehyde
Serum
Drinking Water
Biological Availability
Oral Administration
Arginine
Tail
Arterial Pressure
Cardiovascular Diseases
Therapeutics
Endothelial Cells
Antioxidants

Keywords

  • Hypertension
  • N-nitro-L-arginine methyl ester hydrochloride
  • Nitric oxide
  • Oxidative stress
  • Piper sarmentosum

ASJC Scopus subject areas

  • General

Cite this

Antihypertensive effect of piper sarmentosum in L-NAME-induced hypertensive rats. / Alwi, Nik Aloesnisa Nik Mohd; Zakaria, Zaiton; Karim, Aminuddin Abdul Hamid; Nordin, Nor Anita Megat Mohd; Ugusman, Azizah.

In: Sains Malaysiana, Vol. 47, No. 10, 01.10.2018, p. 2421-2428.

Research output: Contribution to journalArticle

Alwi, Nik Aloesnisa Nik Mohd ; Zakaria, Zaiton ; Karim, Aminuddin Abdul Hamid ; Nordin, Nor Anita Megat Mohd ; Ugusman, Azizah. / Antihypertensive effect of piper sarmentosum in L-NAME-induced hypertensive rats. In: Sains Malaysiana. 2018 ; Vol. 47, No. 10. pp. 2421-2428.
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