Antifungal activity of Andrographis paniculata extracts and active principles against skin pathogenic fungal strains in vitro

Abubakar Sule, Qamar Uddin Ahmed, Jalifah Latip, Othman Abd Samah, Muhammad Nor Omar, Abdulrashid Umar, Bashar Bello S Dogarai

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Context: Andrographis paniculata Nees. (Acanthaceae) is an annual herbaceous plant widely cultivated in southern Asia, China, and Europe. It is used in the treatment of skin infections in India, China, and Malaysia by folk medicine practitioners. Objective: Antifungal activity of the whole plant extracts and isolation of active principles from A. paniculata were investigated. Materials and methods: Dichloromethane (DCM) and methanol (MEOH) extracts of A. paniculata whole plant were screened for their antifungal potential using broth microdilution method in vitro against seven pathogenic fungal species responsible for skin infections. Active principles were detected through bioguided assays and isolated using chromatography techniques. Structures of compounds were elucidated through spectroscopy techniques and comparisons were made with previously reported data for similar compounds. Results: DCM extract revealed lowest minimum inhibitory concentration (MIC) value (100 μg/mL) against Microsporum canis, Candida albicans, and Candida tropicalis, whereas MEOH extract revealed lowest MIC (150 μg/mL) against C. tropicalis and Aspergillus niger. DCM extract showed lowest minimum fungicidal concentration (MFC) value (250 μg/mL) against M. canis, C. albicans, C. tropicalis and A. niger, whereas MEOH extract showed lowest MFC (250 μg/mL) against Trichophyton mentagrophytes, Trichophyton rubrum, M. canis, C. albicans, C. tropicalis and A. niger. Bioassay guided isolation from DCM and MEOH extract afforded 3-O-β-d-glucosyl-14-deoxyandrographiside, 14- deoxyandrographolide, and 14-deoxy-11,12-didehydroandrographolide as antifungal compounds. The lowest MIC (50 μg/mL) and MFC (50 μg/mL) was exerted by 14-deoxyandrographolide on M. canis. Discussion and conclusion: This is first report on the isolation of antifungal substances through bioassay-guided assay from A. paniculata. Our finding justifies the use of A. paniculata in folk medicines for the treatment of fungal skin infections.

Original languageEnglish
Pages (from-to)850-856
Number of pages7
JournalPharmaceutical Biology
Volume50
Issue number7
DOIs
Publication statusPublished - Jul 2012

Fingerprint

Andrographis
Candida tropicalis
Methylene Chloride
Aspergillus niger
Microbial Sensitivity Tests
Candida albicans
Skin
Trichophyton
Traditional Medicine
Biological Assay
China
Acanthaceae
Microsporum
Mycoses
Malaysia
Plant Extracts
Infection
Inhibitory Concentration 50
Methanol
Chromatography

Keywords

  • active principles
  • Andrographis paniculata
  • antifungal activity
  • dermatology
  • MFC
  • MIC
  • skin infections

ASJC Scopus subject areas

  • Drug Discovery
  • Pharmacology
  • Pharmaceutical Science
  • Complementary and alternative medicine
  • Molecular Medicine

Cite this

Antifungal activity of Andrographis paniculata extracts and active principles against skin pathogenic fungal strains in vitro. / Sule, Abubakar; Ahmed, Qamar Uddin; Latip, Jalifah; Samah, Othman Abd; Omar, Muhammad Nor; Umar, Abdulrashid; Dogarai, Bashar Bello S.

In: Pharmaceutical Biology, Vol. 50, No. 7, 07.2012, p. 850-856.

Research output: Contribution to journalArticle

Sule, Abubakar ; Ahmed, Qamar Uddin ; Latip, Jalifah ; Samah, Othman Abd ; Omar, Muhammad Nor ; Umar, Abdulrashid ; Dogarai, Bashar Bello S. / Antifungal activity of Andrographis paniculata extracts and active principles against skin pathogenic fungal strains in vitro. In: Pharmaceutical Biology. 2012 ; Vol. 50, No. 7. pp. 850-856.
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