Antifertility properties of Centella asiatica ethanolic extract as a contraceptive agent

Preliminary study of sperm proteomic

Irfan Yunianto, Nurul Bashah, Mahanem Mat Noor

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objective: To investigate the antifertility properties of Centella asiatica L. ethanolic extract and identify the sperm proteomic changes in treated male rats. Methods: A total of 32 male Sprague-Dawley rats were divided into two groups: Control group (n=16) received distilled water and treatment group (n=16) which received 300 mg/kg of Centella asiatica L. extract for 42 d. After the treatment period, the number of implantation sites was recorded and the sperm proteomic changes were analysed by 2D gel electrophoresis. In addition, the expression of protein spots was quantified by MALDI-TOF analysis. Results: Centella asiatica L. extract resulted in low number of implantation sites in the treatment group (100.00±2.82) compared to the control group (183.00±2.14). The percentage of infertile male rats in the treatment group was higher (43.75%) compared to the control group (18.75%). Proteomic analysis showed the expression of protein spots identified in the treatment group decreased with 234 spots compared to the control group with 282 spots. Conclusions: The results revealed that there was antifertility activity in the male rats with the administration of Centella asiatica L. ethanol extract and the identified proteins could provide understanding on the adverse effect of Centella asiatica in male reproductive system.

Original languageEnglish
Pages (from-to)212-216
Number of pages5
JournalAsian Pacific Journal of Reproduction
Volume6
Issue number5
DOIs
Publication statusPublished - 1 Sep 2017

Fingerprint

antifertility effect
Centella asiatica
contraceptives
Contraceptive Agents
Proteomics
proteomics
Centella
Spermatozoa
spermatozoa
extracts
Control Groups
rats
protein synthesis
Proteins
male reproductive system
Water Purification
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
Electrophoresis, Gel, Two-Dimensional
gel electrophoresis
Sprague Dawley Rats

Keywords

  • Antifertility
  • Centella asiatica L
  • MALDI-TOF
  • Sperm proteomic

ASJC Scopus subject areas

  • Reproductive Medicine
  • Animal Science and Zoology
  • veterinary(all)
  • Plant Science
  • Obstetrics and Gynaecology

Cite this

Antifertility properties of Centella asiatica ethanolic extract as a contraceptive agent : Preliminary study of sperm proteomic. / Yunianto, Irfan; Bashah, Nurul; Mat Noor, Mahanem.

In: Asian Pacific Journal of Reproduction, Vol. 6, No. 5, 01.09.2017, p. 212-216.

Research output: Contribution to journalArticle

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