Penyaringan antikanser ekstrak etanol daripada famili piperaceae terpilih dan penentuannya melalui pewarnaan tripan biru

Translated title of the contribution: Anticancer screening of ethanol extract from selected piperaceae family and its determination via trypan blue staining

Wan Haifa Haryani Wan Omar, Shahrul Hisham Zainal Ariffin, Zaidah Zainal Ariffin, Muhd Fauzi Safian, Sahidan Senafi, Rohaya Megat Abdul Wahab

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The Piperaceae family comprises about 1000 to 2000 of the species that are widely distributed in tropical and subtropical area. In this study, the ethanol extracts were used to evaluate the cytotoxic activity on human hepatocellular carcinoma (HepG2) and non-malignant Chang's liver cell lines by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Ethanol extracts from eight Piperaceae families were selected randomly. Results showed that all eight spesies (P. sarmcntosum, P. ramifilum, P. paucistigmum, P. betle, P. macronatum, P. ridleyi, P. magnibaccum and P. miniaturný showed cytotoxicity activity with Piper sarmcntosum exhibit higher cytotoxicity activity with the IC50 value at 12.5 μg/mL. On the other hand, there were no cytotoxicity activity of Chang's cell that could be induced by the ethanol extracts because the IC50values for non-malignant Chang's cell were greater than 30 μg/mL. Viability analysis using trypan blue showed that P. sarmentosum ethanol extract produced significant (p<0.05) decreased of HepG2 cells as compared to control (p<0.05). In conclusion, all ethanol extracts selected randomly from Piperaceae family showed cytotoxic using MTT assay. Viability analysis using trypan blue staining demonstrates that this type of analysis can become an alternative approach in determining cytotoxicity activity of cells in the presence of extracts.

Original languageUndefined/Unknown
Pages (from-to)941-949
Number of pages9
JournalSains Malaysiana
Volume39
Issue number6
Publication statusPublished - Dec 2010

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Piperaceae
ethanol
screening
cytotoxicity
extracts
viability
cells
Piper
assays
hepatoma
bromides
hepatocytes
inhibitory concentration 50
staining
cell lines

Keywords

  • Cytotoxic
  • Ethanol extract
  • HepG2
  • Piperaceae

ASJC Scopus subject areas

  • General

Cite this

Penyaringan antikanser ekstrak etanol daripada famili piperaceae terpilih dan penentuannya melalui pewarnaan tripan biru. / Omar, Wan Haifa Haryani Wan; Zainal Ariffin, Shahrul Hisham; Ariffin, Zaidah Zainal; Safian, Muhd Fauzi; Senafi, Sahidan; Megat Abdul Wahab, Rohaya.

In: Sains Malaysiana, Vol. 39, No. 6, 12.2010, p. 941-949.

Research output: Contribution to journalArticle

Omar, Wan Haifa Haryani Wan ; Zainal Ariffin, Shahrul Hisham ; Ariffin, Zaidah Zainal ; Safian, Muhd Fauzi ; Senafi, Sahidan ; Megat Abdul Wahab, Rohaya. / Penyaringan antikanser ekstrak etanol daripada famili piperaceae terpilih dan penentuannya melalui pewarnaan tripan biru. In: Sains Malaysiana. 2010 ; Vol. 39, No. 6. pp. 941-949.
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AB - The Piperaceae family comprises about 1000 to 2000 of the species that are widely distributed in tropical and subtropical area. In this study, the ethanol extracts were used to evaluate the cytotoxic activity on human hepatocellular carcinoma (HepG2) and non-malignant Chang's liver cell lines by using MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. Ethanol extracts from eight Piperaceae families were selected randomly. Results showed that all eight spesies (P. sarmcntosum, P. ramifilum, P. paucistigmum, P. betle, P. macronatum, P. ridleyi, P. magnibaccum and P. miniaturný showed cytotoxicity activity with Piper sarmcntosum exhibit higher cytotoxicity activity with the IC50 value at 12.5 μg/mL. On the other hand, there were no cytotoxicity activity of Chang's cell that could be induced by the ethanol extracts because the IC50values for non-malignant Chang's cell were greater than 30 μg/mL. Viability analysis using trypan blue showed that P. sarmentosum ethanol extract produced significant (p<0.05) decreased of HepG2 cells as compared to control (p<0.05). In conclusion, all ethanol extracts selected randomly from Piperaceae family showed cytotoxic using MTT assay. Viability analysis using trypan blue staining demonstrates that this type of analysis can become an alternative approach in determining cytotoxicity activity of cells in the presence of extracts.

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