Antibiotic resistance pattern among escherichia coli O157: H7 isolated from retail imported frozen meat

Nawal Noureldaim Abuelhassan, Sahilah Abd. Mutalib, Nursheila Mustafa Muin, El Mubarak Musa Tibin, Febri Doni, Wan Mohtar Wan Yusoff

Research output: Contribution to journalArticle

Abstract

This present was carried out to isolate and investigate antibiotic resistance pattern among Escherichia coli 0157:H7 strains isolated from imported frozen meat marketed in super markets in Klang valley, Malaysia. A total of 74 samples were purchased from various supermarkets and examined for presence E. coli 0157:H7 using Fluorocult medium which specific for E. coli 0157:H7 and confirmed using API 20E kit. Confirmed isolates were evaluated for antibiotic resistance against 9 antibiotics. Results revealed that 35 out of 126 of the total isolates were confirmed E.coli 0157:H7 with 22 (63%) from beef(10 from country A and 12 from country B), 13 isolates were from buffalo (8 from country A and 5 from country B). Results of antibiotic resistance showed that all isolates were found to be resistant to at least 2 antibiotics and were all resistant to Penicillin G. Resistance was more prevailing against Bacitracin (97.14%), Novobiocin (85.71%), Streptomycin (28.57%), Ampicillin (25.71%), Cephalothin (22.86%), Carbenicillin (17.14%), Sulphamethoxazole (14.29%) and Kanamycin (8.57%). Clustering analysis of the isolated bacteria from the samples using resistance to Cephalothin as base line revealed two clusters with cluster I having 4 sub clusters and cluster II having only 1 cluster. The high prevalence of multidrug resistance among E. coli 0157:H7 pose serious danger to consumers' health. Further study on genetic bases of the antibiotic resistance is hence suggested.

Original languageEnglish
Pages (from-to)739-744
Number of pages6
JournalAsian Journal of Microbiology, Biotechnology and Environmental Sciences
Volume16
Issue number3
Publication statusPublished - 2014

Fingerprint

Escherichia coli O157
antibiotic resistance
Microbial Drug Resistance
meat
Meat
Escherichia coli
Cephalothin
antibiotics
Novobiocin
Anti-Bacterial Agents
Carbenicillin
Bacitracin
Penicillin Resistance
Sulfamethoxazole
Kanamycin
Penicillin G
Buffaloes
Malaysia
Multiple Drug Resistance
Streptomycin

Keywords

  • Antimicrobial resistance
  • Beef
  • Food borne disease
  • Frozen meat
  • Pathogens

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology
  • Microbiology
  • Environmental Science(all)

Cite this

Antibiotic resistance pattern among escherichia coli O157 : H7 isolated from retail imported frozen meat. / Abuelhassan, Nawal Noureldaim; Abd. Mutalib, Sahilah; Muin, Nursheila Mustafa; Tibin, El Mubarak Musa; Doni, Febri; Wan Yusoff, Wan Mohtar.

In: Asian Journal of Microbiology, Biotechnology and Environmental Sciences, Vol. 16, No. 3, 2014, p. 739-744.

Research output: Contribution to journalArticle

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