Antibiotic resistance and plasmid profile of Aeromonas hydrophila isolates from cultured fish, Telapia (Telapia mossambica)

R. Son, G. Rusul, Sahilah Abd. Mutalib, A. Zainuri, A. R. Raha, I. Salmah

Research output: Contribution to journalArticle

78 Citations (Scopus)

Abstract

Strains of Aeromonas, hydrophila isolates from skin lesions of the common freshwater fish, Telapia mossambica, were screened for the presence of plasmid DNA by agarose gel electrophoresis and tested for susceptibility to 10 antimicrobial agents. Of the 21 fish isolates examined, all were resistant to ampicillin and sensitive to gentamycin. Most isolates were resistant to streptomycin (57%), tetracycline (48%) and erythromycin (43%). While seven of 21 isolates harboured plasmids, with sizes ranging from 3 to 63.4 kilobase pair (kb), it was only possible to associate the presence of a plasmid with antibiotic resistance (ampicillin and tetracycline) in strain AH11. Both the plasmid and the associated antimicrobial resistance could be transferred to an Escherichia coli recipient by single-step conjugation at a frequency of 4.3 x 10-3 transconjugants per donor cell.

Original languageEnglish
Pages (from-to)479-482
Number of pages4
JournalLetters in Applied Microbiology
Volume24
Issue number6
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

Aeromonas hydrophila
antibiotic resistance
Antibiotics
Microbial Drug Resistance
plasmid
Fish
Fishes
Plasmids
Anti-Bacterial Agents
Antimicrobial agents
Ampicillin
fish
Tetracycline
Electrophoresis
Escherichia coli
Skin
DNA
Gels
Agar Gel Electrophoresis
Streptomycin

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Water Science and Technology
  • Fluid Flow and Transfer Processes
  • Microbiology
  • Ecology

Cite this

Antibiotic resistance and plasmid profile of Aeromonas hydrophila isolates from cultured fish, Telapia (Telapia mossambica). / Son, R.; Rusul, G.; Abd. Mutalib, Sahilah; Zainuri, A.; Raha, A. R.; Salmah, I.

In: Letters in Applied Microbiology, Vol. 24, No. 6, 1997, p. 479-482.

Research output: Contribution to journalArticle

@article{6dab0907bb3c4bcf81ddd9b1d761bb27,
title = "Antibiotic resistance and plasmid profile of Aeromonas hydrophila isolates from cultured fish, Telapia (Telapia mossambica)",
abstract = "Strains of Aeromonas, hydrophila isolates from skin lesions of the common freshwater fish, Telapia mossambica, were screened for the presence of plasmid DNA by agarose gel electrophoresis and tested for susceptibility to 10 antimicrobial agents. Of the 21 fish isolates examined, all were resistant to ampicillin and sensitive to gentamycin. Most isolates were resistant to streptomycin (57{\%}), tetracycline (48{\%}) and erythromycin (43{\%}). While seven of 21 isolates harboured plasmids, with sizes ranging from 3 to 63.4 kilobase pair (kb), it was only possible to associate the presence of a plasmid with antibiotic resistance (ampicillin and tetracycline) in strain AH11. Both the plasmid and the associated antimicrobial resistance could be transferred to an Escherichia coli recipient by single-step conjugation at a frequency of 4.3 x 10-3 transconjugants per donor cell.",
author = "R. Son and G. Rusul and {Abd. Mutalib}, Sahilah and A. Zainuri and Raha, {A. R.} and I. Salmah",
year = "1997",
language = "English",
volume = "24",
pages = "479--482",
journal = "Letters in Applied Microbiology",
issn = "0266-8254",
publisher = "Wiley-Blackwell",
number = "6",

}

TY - JOUR

T1 - Antibiotic resistance and plasmid profile of Aeromonas hydrophila isolates from cultured fish, Telapia (Telapia mossambica)

AU - Son, R.

AU - Rusul, G.

AU - Abd. Mutalib, Sahilah

AU - Zainuri, A.

AU - Raha, A. R.

AU - Salmah, I.

PY - 1997

Y1 - 1997

N2 - Strains of Aeromonas, hydrophila isolates from skin lesions of the common freshwater fish, Telapia mossambica, were screened for the presence of plasmid DNA by agarose gel electrophoresis and tested for susceptibility to 10 antimicrobial agents. Of the 21 fish isolates examined, all were resistant to ampicillin and sensitive to gentamycin. Most isolates were resistant to streptomycin (57%), tetracycline (48%) and erythromycin (43%). While seven of 21 isolates harboured plasmids, with sizes ranging from 3 to 63.4 kilobase pair (kb), it was only possible to associate the presence of a plasmid with antibiotic resistance (ampicillin and tetracycline) in strain AH11. Both the plasmid and the associated antimicrobial resistance could be transferred to an Escherichia coli recipient by single-step conjugation at a frequency of 4.3 x 10-3 transconjugants per donor cell.

AB - Strains of Aeromonas, hydrophila isolates from skin lesions of the common freshwater fish, Telapia mossambica, were screened for the presence of plasmid DNA by agarose gel electrophoresis and tested for susceptibility to 10 antimicrobial agents. Of the 21 fish isolates examined, all were resistant to ampicillin and sensitive to gentamycin. Most isolates were resistant to streptomycin (57%), tetracycline (48%) and erythromycin (43%). While seven of 21 isolates harboured plasmids, with sizes ranging from 3 to 63.4 kilobase pair (kb), it was only possible to associate the presence of a plasmid with antibiotic resistance (ampicillin and tetracycline) in strain AH11. Both the plasmid and the associated antimicrobial resistance could be transferred to an Escherichia coli recipient by single-step conjugation at a frequency of 4.3 x 10-3 transconjugants per donor cell.

UR - http://www.scopus.com/inward/record.url?scp=0030920174&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030920174&partnerID=8YFLogxK

M3 - Article

VL - 24

SP - 479

EP - 482

JO - Letters in Applied Microbiology

JF - Letters in Applied Microbiology

SN - 0266-8254

IS - 6

ER -