Antibacterial properties of clinacanthus nutans extracts against porphyromonas gingivalis and aggregatibacter actinomycetemcomitans: An in-vitro study

Rohazila Mohamad Hanafiah, Khairine Alia Che Kamaruddin, Nurul Amira Ahmad Saikin, Wan Nur Alwani Binti Wan Abdul Aziz, Muhammad Fahmi Yakop, Vuanghao Lim, Siti Aisyah Abd Ghafar, Nuramirah Azizan, Shahida Mohd Said

Research output: Contribution to journalArticle

Abstract

The aim of this study is to futher investigate and validate the antibacterial effect of Clinacanthus nutans plant extract against periodontal pathogens namely Porphyromonas gingivalis and Aggregatibacter actinomycetamcomitans. Four samples of alcoholic extract of C. nutans leaves were used in different concentrations i.e. 100%, 50%, 10% ethanol and 100% chlorofom and Chlorhexidine 0.2% (CHX) was used as the positive control. The antibacterial activity of C.nutans extract were investigated using disc diffusion agar test for determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). In this study, 50% ethanol extract and 100% chlorofom extract were found to have antibacterial activity against P.gingivalis, while only 50% ethanol crude extract was found to have acceptable antibacterial activity against A. actinomycetamcomitans (p < 0.05). The MIC and MBC tests showed that 50% ethanol extract had bacteriostatic activity against both P.gingivalis and A. actinomycetamcomitans while 100% chlorofom extract had bactericidal activity against P. gingivalis. These two findings were also found to be better than the activity of CHX. C. nutans extract was found to have notable antibacterial activity against P. gingivalis and A. actinomycetamcomitans comparable to CHX 0.2%.

Original languageEnglish
Pages (from-to)401-404
Number of pages4
JournalJournal of International Dental and Medical Research
Volume12
Issue number2
Publication statusPublished - 1 Jan 2019

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Aggregatibacter actinomycetemcomitans
Porphyromonas gingivalis
Chlorhexidine
Ethanol
Microbial Sensitivity Tests
Aggregatibacter
Plant Extracts
Complex Mixtures
Agar
In Vitro Techniques

Keywords

  • Actinomycetamcomitans
  • Clinacanthus nutans
  • Porphorymonas gingivalis & Aggregatibacter

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Antibacterial properties of clinacanthus nutans extracts against porphyromonas gingivalis and aggregatibacter actinomycetemcomitans : An in-vitro study. / Hanafiah, Rohazila Mohamad; Kamaruddin, Khairine Alia Che; Saikin, Nurul Amira Ahmad; Aziz, Wan Nur Alwani Binti Wan Abdul; Yakop, Muhammad Fahmi; Lim, Vuanghao; Ghafar, Siti Aisyah Abd; Azizan, Nuramirah; Said, Shahida Mohd.

In: Journal of International Dental and Medical Research, Vol. 12, No. 2, 01.01.2019, p. 401-404.

Research output: Contribution to journalArticle

Hanafiah, Rohazila Mohamad ; Kamaruddin, Khairine Alia Che ; Saikin, Nurul Amira Ahmad ; Aziz, Wan Nur Alwani Binti Wan Abdul ; Yakop, Muhammad Fahmi ; Lim, Vuanghao ; Ghafar, Siti Aisyah Abd ; Azizan, Nuramirah ; Said, Shahida Mohd. / Antibacterial properties of clinacanthus nutans extracts against porphyromonas gingivalis and aggregatibacter actinomycetemcomitans : An in-vitro study. In: Journal of International Dental and Medical Research. 2019 ; Vol. 12, No. 2. pp. 401-404.
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abstract = "The aim of this study is to futher investigate and validate the antibacterial effect of Clinacanthus nutans plant extract against periodontal pathogens namely Porphyromonas gingivalis and Aggregatibacter actinomycetamcomitans. Four samples of alcoholic extract of C. nutans leaves were used in different concentrations i.e. 100{\%}, 50{\%}, 10{\%} ethanol and 100{\%} chlorofom and Chlorhexidine 0.2{\%} (CHX) was used as the positive control. The antibacterial activity of C.nutans extract were investigated using disc diffusion agar test for determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). In this study, 50{\%} ethanol extract and 100{\%} chlorofom extract were found to have antibacterial activity against P.gingivalis, while only 50{\%} ethanol crude extract was found to have acceptable antibacterial activity against A. actinomycetamcomitans (p < 0.05). The MIC and MBC tests showed that 50{\%} ethanol extract had bacteriostatic activity against both P.gingivalis and A. actinomycetamcomitans while 100{\%} chlorofom extract had bactericidal activity against P. gingivalis. These two findings were also found to be better than the activity of CHX. C. nutans extract was found to have notable antibacterial activity against P. gingivalis and A. actinomycetamcomitans comparable to CHX 0.2{\%}.",
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AU - Aziz, Wan Nur Alwani Binti Wan Abdul

AU - Yakop, Muhammad Fahmi

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AU - Said, Shahida Mohd

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AB - The aim of this study is to futher investigate and validate the antibacterial effect of Clinacanthus nutans plant extract against periodontal pathogens namely Porphyromonas gingivalis and Aggregatibacter actinomycetamcomitans. Four samples of alcoholic extract of C. nutans leaves were used in different concentrations i.e. 100%, 50%, 10% ethanol and 100% chlorofom and Chlorhexidine 0.2% (CHX) was used as the positive control. The antibacterial activity of C.nutans extract were investigated using disc diffusion agar test for determination of the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). In this study, 50% ethanol extract and 100% chlorofom extract were found to have antibacterial activity against P.gingivalis, while only 50% ethanol crude extract was found to have acceptable antibacterial activity against A. actinomycetamcomitans (p < 0.05). The MIC and MBC tests showed that 50% ethanol extract had bacteriostatic activity against both P.gingivalis and A. actinomycetamcomitans while 100% chlorofom extract had bactericidal activity against P. gingivalis. These two findings were also found to be better than the activity of CHX. C. nutans extract was found to have notable antibacterial activity against P. gingivalis and A. actinomycetamcomitans comparable to CHX 0.2%.

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