Antibacterial and DPPH free radical-scavenging activities of methanolic extracts of aaptos sp. (marine sponges)

Habsah Mohamad, Zalilawati Mat Rashid, Khozirah Shaari, Jalifah Latip, Md Nordin Hj Lajis, Abd Manaf Ali

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

This study reports on the evaluation of the antioxidant and antibacterial activities of twelve methanolic extracts (A-L) of Aaptos sp., collected from various locations of Terengganu Islands, namely Pulau Bidong, Pulau Kapas, Pulau Perhentian and Pulau Redang. The antioxidant activity of the twelve specimens was evaluated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method. The antibacterial bioassay against 5 bacteria, i.e. Bacillus subtilis (gram-positive), Escherichia coli (gram-negative), Bacillus proteus, Streptococus agalatea and Streptococus fecalis, was carried out using the disc-diffusion method. In the DPPH method, all extracts exhibited moderate and strong radical scavenging activity when compared to the standards used, i.e. quercetin and butylated hydroxyanisole (BHA) with the inhibition percentage in the range of 55-89%. In particular, specimen H exhibited the strongest radical scavenging activity with IC50 value of 0.1mg/ml. On the contrary, all the specimens showed antibacterial activity at least against one test organism. Interestingly, specimens C, G and L, which were collected from Perhentian, Bidong and Kapas Islands respectively, exhibited weak to strong activity against all bacterial strains. Beside that, specimen F (collected off Redang Island) was weakly bactericidal only against Bacillus proteus. Meanwhile, specimen G (collected off Bidong island) was primarily selected for further isolation to yield cholestan-3β-ol and aaptamine.

Original languageEnglish
Pages (from-to)43-50
Number of pages8
JournalPertanika Journal of Tropical Agricultural Science
Volume32
Issue number1
Publication statusPublished - Feb 2009

Fingerprint

Aaptos
Porifera
Proteus
Bacillus (bacteria)
extracts
butylated hydroxyanisole
Bacillus subtilis
quercetin
inhibitory concentration 50
bioassays
antioxidant activity
methodology
Escherichia coli
antioxidants
2,2-diphenyl-1-picrylhydrazyl
bacteria
organisms
testing

Keywords

  • Aaptamine
  • Aaptos sp
  • Antibacterial
  • Cholestan-3β-ol
  • DPPH free radical scavenging

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Antibacterial and DPPH free radical-scavenging activities of methanolic extracts of aaptos sp. (marine sponges). / Mohamad, Habsah; Rashid, Zalilawati Mat; Shaari, Khozirah; Latip, Jalifah; Lajis, Md Nordin Hj; Ali, Abd Manaf.

In: Pertanika Journal of Tropical Agricultural Science, Vol. 32, No. 1, 02.2009, p. 43-50.

Research output: Contribution to journalArticle

Mohamad, Habsah ; Rashid, Zalilawati Mat ; Shaari, Khozirah ; Latip, Jalifah ; Lajis, Md Nordin Hj ; Ali, Abd Manaf. / Antibacterial and DPPH free radical-scavenging activities of methanolic extracts of aaptos sp. (marine sponges). In: Pertanika Journal of Tropical Agricultural Science. 2009 ; Vol. 32, No. 1. pp. 43-50.
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AB - This study reports on the evaluation of the antioxidant and antibacterial activities of twelve methanolic extracts (A-L) of Aaptos sp., collected from various locations of Terengganu Islands, namely Pulau Bidong, Pulau Kapas, Pulau Perhentian and Pulau Redang. The antioxidant activity of the twelve specimens was evaluated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging method. The antibacterial bioassay against 5 bacteria, i.e. Bacillus subtilis (gram-positive), Escherichia coli (gram-negative), Bacillus proteus, Streptococus agalatea and Streptococus fecalis, was carried out using the disc-diffusion method. In the DPPH method, all extracts exhibited moderate and strong radical scavenging activity when compared to the standards used, i.e. quercetin and butylated hydroxyanisole (BHA) with the inhibition percentage in the range of 55-89%. In particular, specimen H exhibited the strongest radical scavenging activity with IC50 value of 0.1mg/ml. On the contrary, all the specimens showed antibacterial activity at least against one test organism. Interestingly, specimens C, G and L, which were collected from Perhentian, Bidong and Kapas Islands respectively, exhibited weak to strong activity against all bacterial strains. Beside that, specimen F (collected off Redang Island) was weakly bactericidal only against Bacillus proteus. Meanwhile, specimen G (collected off Bidong island) was primarily selected for further isolation to yield cholestan-3β-ol and aaptamine.

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