Kajian aktiviti antibakteria dan antioksidan liken Usnea rubrotincta, Ramalina dumeticola, Cladonia verticillata dan sebatian kimianya

Translated title of the contribution: Antibacterial and antioxidant activity of lichens: Usnea rubrotincta, Ramalina dumeticola, Cladonia verticillata and their chemical constituents

Saranyapiriya Gunasekaran, Vinoshene Pillai Rajan, Surash Ramanathan, Vikneswaran Murugaiyah, Mohd Wahid Samsudin, Laily B. Din

    Research output: Contribution to journalArticle

    4 Citations (Scopus)

    Abstract

    The present study was carried out to evaluate the antibacterial and antioxidant activity of extract and chemical constituents of Usnea rubrotincta, Ramalina dumeticola and Cladonia verticillata. Acetone extract of U. rubrotincta showed promising antibacterial activity against Gram positive bacteria Bacillus subtilis with the lowest Minimal Inhibitory Concentration (MIC) value of (15.63 μg/mL). Six secondary metabolites were isolated using Preparative High Performance Liquid Chromatography (PHPLC) method from the two bioactive lichens U. rubrotincta and R. dumeticola (compound 1 – 6). Among all six compounds, compound (1) exhibited strongest activity against both the tested Gram positive bacteria at 7.81 μg/mL. Compound (6) had 69.57% scavenging activity against DPPH free radical while the rest only showed below 50% scavenging activity. This is the first evaluation of antibacterial activity of lichens found in Malaysia and to our knowledge, this is the first report of antibacterial and antioxidant activity of compound (3) and (5).

    Original languageMalay
    Pages (from-to)1-13
    Number of pages13
    JournalMalaysian Journal of Analytical Sciences
    Volume20
    Issue number1
    Publication statusPublished - 2016

    Fingerprint

    Scavenging
    Bacteria
    Antioxidants
    High performance liquid chromatography
    Bacilli
    Metabolites
    Acetone
    Free Radicals
    Lichens

    ASJC Scopus subject areas

    • Analytical Chemistry

    Cite this

    Gunasekaran, S., Rajan, V. P., Ramanathan, S., Murugaiyah, V., Samsudin, M. W., & Din, L. B. (2016). Kajian aktiviti antibakteria dan antioksidan liken Usnea rubrotincta, Ramalina dumeticola, Cladonia verticillata dan sebatian kimianya. Malaysian Journal of Analytical Sciences, 20(1), 1-13.

    Kajian aktiviti antibakteria dan antioksidan liken Usnea rubrotincta, Ramalina dumeticola, Cladonia verticillata dan sebatian kimianya. / Gunasekaran, Saranyapiriya; Rajan, Vinoshene Pillai; Ramanathan, Surash; Murugaiyah, Vikneswaran; Samsudin, Mohd Wahid; Din, Laily B.

    In: Malaysian Journal of Analytical Sciences, Vol. 20, No. 1, 2016, p. 1-13.

    Research output: Contribution to journalArticle

    Gunasekaran, S, Rajan, VP, Ramanathan, S, Murugaiyah, V, Samsudin, MW & Din, LB 2016, 'Kajian aktiviti antibakteria dan antioksidan liken Usnea rubrotincta, Ramalina dumeticola, Cladonia verticillata dan sebatian kimianya', Malaysian Journal of Analytical Sciences, vol. 20, no. 1, pp. 1-13.
    Gunasekaran, Saranyapiriya ; Rajan, Vinoshene Pillai ; Ramanathan, Surash ; Murugaiyah, Vikneswaran ; Samsudin, Mohd Wahid ; Din, Laily B. / Kajian aktiviti antibakteria dan antioksidan liken Usnea rubrotincta, Ramalina dumeticola, Cladonia verticillata dan sebatian kimianya. In: Malaysian Journal of Analytical Sciences. 2016 ; Vol. 20, No. 1. pp. 1-13.
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    abstract = "The present study was carried out to evaluate the antibacterial and antioxidant activity of extract and chemical constituents of Usnea rubrotincta, Ramalina dumeticola and Cladonia verticillata. Acetone extract of U. rubrotincta showed promising antibacterial activity against Gram positive bacteria Bacillus subtilis with the lowest Minimal Inhibitory Concentration (MIC) value of (15.63 μg/mL). Six secondary metabolites were isolated using Preparative High Performance Liquid Chromatography (PHPLC) method from the two bioactive lichens U. rubrotincta and R. dumeticola (compound 1 – 6). Among all six compounds, compound (1) exhibited strongest activity against both the tested Gram positive bacteria at 7.81 μg/mL. Compound (6) had 69.57{\%} scavenging activity against DPPH free radical while the rest only showed below 50{\%} scavenging activity. This is the first evaluation of antibacterial activity of lichens found in Malaysia and to our knowledge, this is the first report of antibacterial and antioxidant activity of compound (3) and (5).",
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