Antibacterial activity of Myristica fragrans against oral pathogens

Zaleha Shafiei, Nadia Najwa Shuhairi, Nordiyana Md Fazly Shah Yap, Carrie Anne Harry Sibungkil, Jalifah Latip

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Myristica fragrans Houtt is mostly cultivated for spices in Penang Island, Malaysia. The ethyl acetate and ethanol extracts of flesh, mace and seed of Myristica fragrans was evaluated the bactericidal potential against three Gram-positive cariogenic bacteria (Streptococcus mutans ATCC 25175, Streptococcus mitis ATCC 6249, and Streptococcus salivarius ATCC 13419) and three Gram-negative periodontopathic bacteria (Aggregatibacter actinomycetemcomitans ATCC 29522, Porphyromonas gingivalis ATCC 33277, and Fusobacterium nucleatum ATCC 25586). Antibacterial activities of the extracts was determined by twofold serial microdilution, with minimum inhibitory concentrations (MIC) ranging from 1.25 to 640 mg/mL and 0.075 to 40 mg/mL. The minimum bactericidal concentration (MBC) was obtained by subculturing method. Among all extracts tested, ethyl acetate extract of flesh has the highest significant inhibitory effects against Gram-positive and Gram-negative bacteria with mean MIC value ranging from 0.625 to 1. 25 ± 0. 00 (SD) mg/mL; P = 0. 017) and highest bactericidal effects at mean MBC value ranging from 0.625 mg/mL to 20 ± 0. 00 (SD) mg/mL. While for seed and mace of Myristica fragrans, their ethanol extracts exhibited good antibacterial activity against both groups of test pathogens compared to its ethyl acetate extracts. All of the extracts of Myristica fragrans did not show any antibacterial activities against Fusobacterium nucleatum ATCC 25586. Thus, our study showed the potential effect of ethyl acetate and ethanol extracts from flesh, seed and mace of Myristica fragrans to be new natural agent that can be incorporated in oral care products.

Original languageEnglish
Article number825362
JournalEvidence-based Complementary and Alternative Medicine
Volume2012
DOIs
Publication statusPublished - 2012

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Myristica fragrans
omega-Chloroacetophenone
Fusobacterium nucleatum
Seeds
Ethanol
Microbial Sensitivity Tests
Gram-Negative Bacteria
Streptococcus mitis
Aggregatibacter actinomycetemcomitans
Spices
Porphyromonas gingivalis
Streptococcus mutans
Malaysia
Gram-Positive Bacteria
Islands
ethyl acetate

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

Antibacterial activity of Myristica fragrans against oral pathogens. / Shafiei, Zaleha; Shuhairi, Nadia Najwa; Md Fazly Shah Yap, Nordiyana; Harry Sibungkil, Carrie Anne; Latip, Jalifah.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2012, 825362, 2012.

Research output: Contribution to journalArticle

Shafiei, Zaleha ; Shuhairi, Nadia Najwa ; Md Fazly Shah Yap, Nordiyana ; Harry Sibungkil, Carrie Anne ; Latip, Jalifah. / Antibacterial activity of Myristica fragrans against oral pathogens. In: Evidence-based Complementary and Alternative Medicine. 2012 ; Vol. 2012.
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