Antibacterial activity of biosynthesized gold nanoparticles using biomolecules from Lignosus rhinocerotis and chitosan

Haliza Katas, Chei Sin Lim, Ahmad Yasser Hamdi Nor Azlan, Fhataheya Buang, Mohd Fauzi Mh Busra

Research output: Contribution to journalArticle

Abstract

A simple, cost-effective, and environmentally friendly method is needed for synthesizing metal nanoparticles, including gold nanoparticles (AuNPs). In this study, AuNPs were synthesized with Lignosus rhinocerotis sclerotial extract (LRE) and chitosan (CS) as reducing and stabilizing agents, respectively. Different LRE concentrations from cold and hot water extraction (CWE and HWE, respectively) were used to reduce chloroauric acid (HAuCl4) to form AuNPs. Positively charged chitosan stabilized AuNPs (CS-AuNPs) mediated by LRE exhibited a surface plasmon resonance (SPR) band at 533 nm. The CS-AuNPs synthesized using CWE had a smaller particle size (49.5 ± 6.7–82.4 ± 28.0 nm) compared to that of the HWE samples (80.3 ± 23.4–125.3 ± 41.5 nm), depending on LRE concentration. FTIR results suggested protein and polysaccharides in LRE were the sources of reducing power, reducing gold ions to AuNPs. CS-AuNPs were mostly spherical with higher LRE concentrations, whereas some triangular, pentagonal, irregular, and rod shaped AuNPs were observed at lower LRE concentrations. CS-AuNPs mediated by LRE displayed effective antibacterial activity against gram-negative (Pseudomonas aeruginosa and Escherichia coli) and gram-positive bacteria (Staphylococcus aureus and Bacillus sp.). Thus, the biosynthesized AuNPs using LRE and chitosan provide opportunities for developing stable and eco-friendly nanoparticles with effective antibacterial properties.

Original languageEnglish
Pages (from-to)283-292
Number of pages10
JournalSaudi Pharmaceutical Journal
Volume27
Issue number2
DOIs
Publication statusPublished - 1 Feb 2019

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Chitosan
Gold
Nanoparticles
Metal Nanoparticles
Electric Power Supplies
Surface Plasmon Resonance
Excipients
Reducing Agents
Gram-Positive Bacteria
Fourier Transform Infrared Spectroscopy
Particle Size
Pseudomonas aeruginosa
Bacillus
Polysaccharides
Staphylococcus aureus
Ions
Escherichia coli
Costs and Cost Analysis
Water
Proteins

Keywords

  • Antimicrobial
  • Gold nanoparticles
  • Green synthesis
  • Lignosus rhinocerotis
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

Cite this

Antibacterial activity of biosynthesized gold nanoparticles using biomolecules from Lignosus rhinocerotis and chitosan. / Katas, Haliza; Lim, Chei Sin; Nor Azlan, Ahmad Yasser Hamdi; Buang, Fhataheya; Mh Busra, Mohd Fauzi.

In: Saudi Pharmaceutical Journal, Vol. 27, No. 2, 01.02.2019, p. 283-292.

Research output: Contribution to journalArticle

Katas, Haliza ; Lim, Chei Sin ; Nor Azlan, Ahmad Yasser Hamdi ; Buang, Fhataheya ; Mh Busra, Mohd Fauzi. / Antibacterial activity of biosynthesized gold nanoparticles using biomolecules from Lignosus rhinocerotis and chitosan. In: Saudi Pharmaceutical Journal. 2019 ; Vol. 27, No. 2. pp. 283-292.
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