Anti-urolithiatic terpenoid compound from Plantago major Linn. (Ekor Anjing)

A. A. Sharifa, J. Jamaludin, L. S. Kiong, L. A. Chia, Khairul Osman

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The aim of this study was to determine the inhibition effects of the terpenoid of Plantago major on calcium oxalate crystals in vitro and to compare the effects of Plantago major with clinically used drugs like zyloric and potassium citrate for the treatment of urinary stone. Modified Schneider slide gel method was used for in vitro study and crystals formed were measured by Image Analyser System (Leica) after 24 h of treatment. The active compound in the methanol extract of Plantago major was isolated by bioassay - guided fractionation & isolation method. Dimethylsulphoxide (DMSO) was used as the negative control and zyloric and potassium citrate were used as positive controls. The results showed that crude methanol extract of Plantago major contained the active compound terpenoid. Terpenoid, zyloric and potassium citrate at concentrations in the range of (100 μg/mL - 250 μg/mL) signifcantly inhibited the area of crystal formation in comparison to the negative control after 24 h (p<0.001). The Zyloric and terpenoid of Plantago major in the concentrations of (100 μg/mL-250 μg/mL) inhibited the sizes of crystals signifcantly (p<0.05). Potassium citrate was more effective, than terpenoid of Plantago major in inhibiting the size of crystals at two concentrations i.e. 100 μg/mL and 150 μg/mL respectively (p<0.05). However the IC 50 values for terpenoid of Plantago major, potassium citrate and zyloric were 250 μg/mL, 300 μg/mL and 550 μg/mL, respectively. The inhibition effect of the terpenoid of Plantago major extract on crystal size was much better than Zyloric and potassium citrate.

Original languageEnglish
Pages (from-to)33-39
Number of pages7
JournalSains Malaysiana
Volume41
Issue number1
Publication statusPublished - Jan 2012

Fingerprint

Plantago major
terpenoids
citrates
crystals
potassium
extracts
methanol
urinary calculi
calcium oxalate
isolation techniques
dimethyl sulfoxide
in vitro studies
inhibitory concentration 50
fractionation
bioassays
gels
drugs

Keywords

  • Calcium oxalate crystals
  • Inhibition effects
  • Plantago major
  • Terpenoid

ASJC Scopus subject areas

  • General

Cite this

Sharifa, A. A., Jamaludin, J., Kiong, L. S., Chia, L. A., & Osman, K. (2012). Anti-urolithiatic terpenoid compound from Plantago major Linn. (Ekor Anjing). Sains Malaysiana, 41(1), 33-39.

Anti-urolithiatic terpenoid compound from Plantago major Linn. (Ekor Anjing). / Sharifa, A. A.; Jamaludin, J.; Kiong, L. S.; Chia, L. A.; Osman, Khairul.

In: Sains Malaysiana, Vol. 41, No. 1, 01.2012, p. 33-39.

Research output: Contribution to journalArticle

Sharifa, AA, Jamaludin, J, Kiong, LS, Chia, LA & Osman, K 2012, 'Anti-urolithiatic terpenoid compound from Plantago major Linn. (Ekor Anjing)', Sains Malaysiana, vol. 41, no. 1, pp. 33-39.
Sharifa AA, Jamaludin J, Kiong LS, Chia LA, Osman K. Anti-urolithiatic terpenoid compound from Plantago major Linn. (Ekor Anjing). Sains Malaysiana. 2012 Jan;41(1):33-39.
Sharifa, A. A. ; Jamaludin, J. ; Kiong, L. S. ; Chia, L. A. ; Osman, Khairul. / Anti-urolithiatic terpenoid compound from Plantago major Linn. (Ekor Anjing). In: Sains Malaysiana. 2012 ; Vol. 41, No. 1. pp. 33-39.
@article{44f0f8909f474f22a5df157826e19b4b,
title = "Anti-urolithiatic terpenoid compound from Plantago major Linn. (Ekor Anjing)",
abstract = "The aim of this study was to determine the inhibition effects of the terpenoid of Plantago major on calcium oxalate crystals in vitro and to compare the effects of Plantago major with clinically used drugs like zyloric and potassium citrate for the treatment of urinary stone. Modified Schneider slide gel method was used for in vitro study and crystals formed were measured by Image Analyser System (Leica) after 24 h of treatment. The active compound in the methanol extract of Plantago major was isolated by bioassay - guided fractionation & isolation method. Dimethylsulphoxide (DMSO) was used as the negative control and zyloric and potassium citrate were used as positive controls. The results showed that crude methanol extract of Plantago major contained the active compound terpenoid. Terpenoid, zyloric and potassium citrate at concentrations in the range of (100 μg/mL - 250 μg/mL) signifcantly inhibited the area of crystal formation in comparison to the negative control after 24 h (p<0.001). The Zyloric and terpenoid of Plantago major in the concentrations of (100 μg/mL-250 μg/mL) inhibited the sizes of crystals signifcantly (p<0.05). Potassium citrate was more effective, than terpenoid of Plantago major in inhibiting the size of crystals at two concentrations i.e. 100 μg/mL and 150 μg/mL respectively (p<0.05). However the IC 50 values for terpenoid of Plantago major, potassium citrate and zyloric were 250 μg/mL, 300 μg/mL and 550 μg/mL, respectively. The inhibition effect of the terpenoid of Plantago major extract on crystal size was much better than Zyloric and potassium citrate.",
keywords = "Calcium oxalate crystals, Inhibition effects, Plantago major, Terpenoid",
author = "Sharifa, {A. A.} and J. Jamaludin and Kiong, {L. S.} and Chia, {L. A.} and Khairul Osman",
year = "2012",
month = "1",
language = "English",
volume = "41",
pages = "33--39",
journal = "Sains Malaysiana",
issn = "0126-6039",
publisher = "Penerbit Universiti Kebangsaan Malaysia",
number = "1",

}

TY - JOUR

T1 - Anti-urolithiatic terpenoid compound from Plantago major Linn. (Ekor Anjing)

AU - Sharifa, A. A.

AU - Jamaludin, J.

AU - Kiong, L. S.

AU - Chia, L. A.

AU - Osman, Khairul

PY - 2012/1

Y1 - 2012/1

N2 - The aim of this study was to determine the inhibition effects of the terpenoid of Plantago major on calcium oxalate crystals in vitro and to compare the effects of Plantago major with clinically used drugs like zyloric and potassium citrate for the treatment of urinary stone. Modified Schneider slide gel method was used for in vitro study and crystals formed were measured by Image Analyser System (Leica) after 24 h of treatment. The active compound in the methanol extract of Plantago major was isolated by bioassay - guided fractionation & isolation method. Dimethylsulphoxide (DMSO) was used as the negative control and zyloric and potassium citrate were used as positive controls. The results showed that crude methanol extract of Plantago major contained the active compound terpenoid. Terpenoid, zyloric and potassium citrate at concentrations in the range of (100 μg/mL - 250 μg/mL) signifcantly inhibited the area of crystal formation in comparison to the negative control after 24 h (p<0.001). The Zyloric and terpenoid of Plantago major in the concentrations of (100 μg/mL-250 μg/mL) inhibited the sizes of crystals signifcantly (p<0.05). Potassium citrate was more effective, than terpenoid of Plantago major in inhibiting the size of crystals at two concentrations i.e. 100 μg/mL and 150 μg/mL respectively (p<0.05). However the IC 50 values for terpenoid of Plantago major, potassium citrate and zyloric were 250 μg/mL, 300 μg/mL and 550 μg/mL, respectively. The inhibition effect of the terpenoid of Plantago major extract on crystal size was much better than Zyloric and potassium citrate.

AB - The aim of this study was to determine the inhibition effects of the terpenoid of Plantago major on calcium oxalate crystals in vitro and to compare the effects of Plantago major with clinically used drugs like zyloric and potassium citrate for the treatment of urinary stone. Modified Schneider slide gel method was used for in vitro study and crystals formed were measured by Image Analyser System (Leica) after 24 h of treatment. The active compound in the methanol extract of Plantago major was isolated by bioassay - guided fractionation & isolation method. Dimethylsulphoxide (DMSO) was used as the negative control and zyloric and potassium citrate were used as positive controls. The results showed that crude methanol extract of Plantago major contained the active compound terpenoid. Terpenoid, zyloric and potassium citrate at concentrations in the range of (100 μg/mL - 250 μg/mL) signifcantly inhibited the area of crystal formation in comparison to the negative control after 24 h (p<0.001). The Zyloric and terpenoid of Plantago major in the concentrations of (100 μg/mL-250 μg/mL) inhibited the sizes of crystals signifcantly (p<0.05). Potassium citrate was more effective, than terpenoid of Plantago major in inhibiting the size of crystals at two concentrations i.e. 100 μg/mL and 150 μg/mL respectively (p<0.05). However the IC 50 values for terpenoid of Plantago major, potassium citrate and zyloric were 250 μg/mL, 300 μg/mL and 550 μg/mL, respectively. The inhibition effect of the terpenoid of Plantago major extract on crystal size was much better than Zyloric and potassium citrate.

KW - Calcium oxalate crystals

KW - Inhibition effects

KW - Plantago major

KW - Terpenoid

UR - http://www.scopus.com/inward/record.url?scp=84055193554&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84055193554&partnerID=8YFLogxK

M3 - Article

VL - 41

SP - 33

EP - 39

JO - Sains Malaysiana

JF - Sains Malaysiana

SN - 0126-6039

IS - 1

ER -