Anti-TNF-α and anti-arthritic effect of patuletin

A rare flavonoid from Tagetes patula

Almas Jabeen, Osman Mesaik Mohammed Ahmed Hassan, Shabana U. Simjee, Lubna, Samina Bano, Shaheen Faizi

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Rheumatoid arthritis (RA) poses a serious health problem as a chronic autoimmune joint disease with significant mortality and morbidity. Proinflammatory cytokines TNF-α and IL-1β, reactive oxygen species (ROS), and activated CD4+ T-cells play key roles in the progression of arthritis. The aim of the study is to evaluate the in vitro and in vivo immunomodulatory and anti-arthritic effect of flavonoid patuletin, isolated from Tagetes patula. ELISA was applied for quantification of TNF-α and IL-1β. Intracellular and extracellular ROS production from phagocytes was measured by the chemiluminescence technique. Proliferation of T-cells was observed using a liquid scintillation counter. Cytotoxicity was assessed by a MTT assay. The serological and histological analysis studies were performed using a rodent model of adjuvant-induced arthritis (AIA). Expression of p38 and NF-κB after treatment of compound was observed by western blotting. Patuletin showed potent inhibitory effects on TNF-α in vitro as well as inhibited the production of both cytokines in vivo. It also showed potent suppression of proliferation of T-cells and significantly inhibited the extracellular and intracellular ROS production. Patuletin revealed significant anti-inflammatory and anti-arthritic activities in the rodent model of adjuvant-induced arthritis (AIA). Histologically, it causes mild bone destruction compared to the arthritic control group, thus representing its anti-arthritic potential. Based on these studies, patuletin could be considered as a potential immunosuppressive and anti-arthritic lead candidate.

Original languageEnglish
Pages (from-to)232-240
Number of pages9
JournalInternational Immunopharmacology
Volume36
DOIs
Publication statusPublished - 1 Jul 2016
Externally publishedYes

Fingerprint

Tagetes
Flavonoids
Arthritis
Reactive Oxygen Species
Experimental Arthritis
T-Lymphocytes
Interleukin-1
Rodentia
Cytokines
Scintillation Counting
Joint Diseases
Immunosuppressive Agents
Phagocytes
Luminescence
Autoimmune Diseases
patuletin
Rheumatoid Arthritis
Anti-Inflammatory Agents
Western Blotting
Enzyme-Linked Immunosorbent Assay

Keywords

  • Anti TNF-α
  • Immunosuppressive
  • Patuletin
  • Rheumatoid arthritis

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Pharmacology

Cite this

Anti-TNF-α and anti-arthritic effect of patuletin : A rare flavonoid from Tagetes patula. / Jabeen, Almas; Mohammed Ahmed Hassan, Osman Mesaik; Simjee, Shabana U.; Lubna; Bano, Samina; Faizi, Shaheen.

In: International Immunopharmacology, Vol. 36, 01.07.2016, p. 232-240.

Research output: Contribution to journalArticle

Jabeen, Almas ; Mohammed Ahmed Hassan, Osman Mesaik ; Simjee, Shabana U. ; Lubna ; Bano, Samina ; Faizi, Shaheen. / Anti-TNF-α and anti-arthritic effect of patuletin : A rare flavonoid from Tagetes patula. In: International Immunopharmacology. 2016 ; Vol. 36. pp. 232-240.
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