Abstract
Background: The potential of secondary metabolites extracted from Streptomyces sp. to treat bacterial infections including infections with Staphylococcus aureus is previously documented. The current study showed significant antimicrobial activities associated with endophytic Streptomyces sp. isolated from medicinal plants in Peninsular Malaysia. Objectives: The current study aimed to determine anti-methicillin-resistant-Staphylococcus aureus (MRSA) activities of Streptomyces sp. isolates. Materials and Methods: Disc difusion and Minimum Inhibitory Concentration (MIC) assay were used to determine the antibacterial activity of Streptomyces sp. isolates. Optimization of fermentation parameters for the most potent anti-MRSA extract in terms of medium type, pH, aeration rate, and culture period was also carried out. Lastly, toxicity of the extract against Chang liver cells was determined employing the MTT, 2- (3, 5- diphenyltetrazol-2-ium-2-yl) -4, 5-dimethyl -1, 3 - thiazole; bromide assay. Results: The results indicated Streptomyces sp. SUK 25 isolates showed the most potent anti-MRSA activity. Disc difusion assay revealed that spread plate technique was more efficient in screening anti-MRSA activity compared to pour plate (P < 0.05). To determine anti–MRSA MIC of Streptomyces sp. SUK 25, Thronton media was used. Therefore, MIC was determined as 2.44 ± 0.01 µg/mL, and accordingly, the lowest MIC was 1.95 µg/mL based on a seven-day culture, pH7, and aeration rate of 140 rpm. The crude extract was not toxic against Chang liver cells (IC<inf>50</inf> = 43.31 ± 1.24 µg/mL). Conclusions: The Streptomyces sp. SUK 25 culturing was optimized using Thronton media, at pH 7 and aeration of 140 rpm. Further isolation and identification of bioactive compounds will develop anti-MRSA therapeutics.
| Original language | English |
|---|---|
| Article number | e16784 |
| Pages (from-to) | 1-7 |
| Number of pages | 7 |
| Journal | Jundishapur Journal of Microbiology |
| Volume | 8 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 2015 |
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Keywords
- Culture
- MRSA
- Streptomyces sp
ASJC Scopus subject areas
- Infectious Diseases
- Microbiology
- Microbiology (medical)
Cite this
Anti-methicillin resistant staphylococcus aureus activity and optimal culture condition of streptomyces sp. SUK 25. / Junaidah, Ahmad Siti; Suhaini, Sudi; Mohd. Sidek, Hasidah; Basri, Dayang Fredalina; Mohamad Zin, Noraziah.
In: Jundishapur Journal of Microbiology, Vol. 8, No. 5, e16784, 2015, p. 1-7.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Anti-methicillin resistant staphylococcus aureus activity and optimal culture condition of streptomyces sp. SUK 25
AU - Junaidah, Ahmad Siti
AU - Suhaini, Sudi
AU - Mohd. Sidek, Hasidah
AU - Basri, Dayang Fredalina
AU - Mohamad Zin, Noraziah
PY - 2015
Y1 - 2015
N2 - Background: The potential of secondary metabolites extracted from Streptomyces sp. to treat bacterial infections including infections with Staphylococcus aureus is previously documented. The current study showed significant antimicrobial activities associated with endophytic Streptomyces sp. isolated from medicinal plants in Peninsular Malaysia. Objectives: The current study aimed to determine anti-methicillin-resistant-Staphylococcus aureus (MRSA) activities of Streptomyces sp. isolates. Materials and Methods: Disc difusion and Minimum Inhibitory Concentration (MIC) assay were used to determine the antibacterial activity of Streptomyces sp. isolates. Optimization of fermentation parameters for the most potent anti-MRSA extract in terms of medium type, pH, aeration rate, and culture period was also carried out. Lastly, toxicity of the extract against Chang liver cells was determined employing the MTT, 2- (3, 5- diphenyltetrazol-2-ium-2-yl) -4, 5-dimethyl -1, 3 - thiazole; bromide assay. Results: The results indicated Streptomyces sp. SUK 25 isolates showed the most potent anti-MRSA activity. Disc difusion assay revealed that spread plate technique was more efficient in screening anti-MRSA activity compared to pour plate (P < 0.05). To determine anti–MRSA MIC of Streptomyces sp. SUK 25, Thronton media was used. Therefore, MIC was determined as 2.44 ± 0.01 µg/mL, and accordingly, the lowest MIC was 1.95 µg/mL based on a seven-day culture, pH7, and aeration rate of 140 rpm. The crude extract was not toxic against Chang liver cells (IC50 = 43.31 ± 1.24 µg/mL). Conclusions: The Streptomyces sp. SUK 25 culturing was optimized using Thronton media, at pH 7 and aeration of 140 rpm. Further isolation and identification of bioactive compounds will develop anti-MRSA therapeutics.
AB - Background: The potential of secondary metabolites extracted from Streptomyces sp. to treat bacterial infections including infections with Staphylococcus aureus is previously documented. The current study showed significant antimicrobial activities associated with endophytic Streptomyces sp. isolated from medicinal plants in Peninsular Malaysia. Objectives: The current study aimed to determine anti-methicillin-resistant-Staphylococcus aureus (MRSA) activities of Streptomyces sp. isolates. Materials and Methods: Disc difusion and Minimum Inhibitory Concentration (MIC) assay were used to determine the antibacterial activity of Streptomyces sp. isolates. Optimization of fermentation parameters for the most potent anti-MRSA extract in terms of medium type, pH, aeration rate, and culture period was also carried out. Lastly, toxicity of the extract against Chang liver cells was determined employing the MTT, 2- (3, 5- diphenyltetrazol-2-ium-2-yl) -4, 5-dimethyl -1, 3 - thiazole; bromide assay. Results: The results indicated Streptomyces sp. SUK 25 isolates showed the most potent anti-MRSA activity. Disc difusion assay revealed that spread plate technique was more efficient in screening anti-MRSA activity compared to pour plate (P < 0.05). To determine anti–MRSA MIC of Streptomyces sp. SUK 25, Thronton media was used. Therefore, MIC was determined as 2.44 ± 0.01 µg/mL, and accordingly, the lowest MIC was 1.95 µg/mL based on a seven-day culture, pH7, and aeration rate of 140 rpm. The crude extract was not toxic against Chang liver cells (IC50 = 43.31 ± 1.24 µg/mL). Conclusions: The Streptomyces sp. SUK 25 culturing was optimized using Thronton media, at pH 7 and aeration of 140 rpm. Further isolation and identification of bioactive compounds will develop anti-MRSA therapeutics.
KW - Culture
KW - MRSA
KW - Streptomyces sp
UR - http://www.scopus.com/inward/record.url?scp=84930392324&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84930392324&partnerID=8YFLogxK
U2 - 10.5812/jjm.16784
DO - 10.5812/jjm.16784
M3 - Article
AN - SCOPUS:84930392324
VL - 8
SP - 1
EP - 7
JO - Jundishapur Journal of Microbiology
JF - Jundishapur Journal of Microbiology
SN - 2008-3645
IS - 5
M1 - e16784
ER -