Anti-inflammatory property of Parkia speciosa empty pod extract in human umbilical vein endothelial cells

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Parkia speciosa Hassk, locally known as petai papan, is a common medicinal plant found in Southeast Asia. Its empty pods were reported to contain high concentrations of polyphenols, particularly quercetin. This study aimed to evaluate the anti-inflammatory properties of P. speciosa empty pod extract in human umbilical vein endothelial cells (HUVECs). The empty pods were extracted by sequential fractionation with absolute ethanol and ethyl acetate. HUVECs were divided into four groups. HUVECs were exposed to tumor necrosis factor-α (TNF-α, 10 ng/mL) in the presence (25 μg/mL) or absence of Parkia speciosa extract. Quercetin (125 μM) served as the positive control, while another group that was not exposed to TNF-α acted as the negative control. The protein expressions of the inflammatory mediators, which were NF-κB p65, iNOS, COX-2 and VCAM-1, were significantly decreased in the P. speciosa and quercetin groups exposed to TNF-α. P. speciosa extract and quercetin also significantly reduced intracellular reactive oxygen species and nitric oxide levels as well as inducible nitric oxide synthase (iNOS) activity caused by TNF-α in HUVECs. In conclusion, P. speciosa empty pod extract exhibited potential anti-inflammatory properties against TNF-α-induced inflammation, possibly by modulating the NF-κB p65 pathway. The effects were comparable to that of quercetin.

Original languageEnglish
Pages (from-to)152-158
Number of pages7
JournalJournal of Applied Pharmaceutical Science
Volume8
Issue number1
DOIs
Publication statusPublished - 1 Jan 2018

Fingerprint

Quercetin
Human Umbilical Vein Endothelial Cells
Anti-Inflammatory Agents
Nitric Oxide Synthase Type II
Southeastern Asia
Vascular Cell Adhesion Molecule-1
Polyphenols
Medicinal Plants
Reactive Oxygen Species
Nitric Oxide
Ethanol
Tumor Necrosis Factor-alpha
Inflammation
Proteins

Keywords

  • Cyclooxygenase-2
  • Inducible nitric oxide synthase
  • NF-κB p65
  • Reactive oxygen species
  • Vascular cell adhesion molecule

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Pharmacology (medical)

Cite this

@article{7518f7b05bba4906b1b50b78e49f8b11,
title = "Anti-inflammatory property of Parkia speciosa empty pod extract in human umbilical vein endothelial cells",
abstract = "Parkia speciosa Hassk, locally known as petai papan, is a common medicinal plant found in Southeast Asia. Its empty pods were reported to contain high concentrations of polyphenols, particularly quercetin. This study aimed to evaluate the anti-inflammatory properties of P. speciosa empty pod extract in human umbilical vein endothelial cells (HUVECs). The empty pods were extracted by sequential fractionation with absolute ethanol and ethyl acetate. HUVECs were divided into four groups. HUVECs were exposed to tumor necrosis factor-α (TNF-α, 10 ng/mL) in the presence (25 μg/mL) or absence of Parkia speciosa extract. Quercetin (125 μM) served as the positive control, while another group that was not exposed to TNF-α acted as the negative control. The protein expressions of the inflammatory mediators, which were NF-κB p65, iNOS, COX-2 and VCAM-1, were significantly decreased in the P. speciosa and quercetin groups exposed to TNF-α. P. speciosa extract and quercetin also significantly reduced intracellular reactive oxygen species and nitric oxide levels as well as inducible nitric oxide synthase (iNOS) activity caused by TNF-α in HUVECs. In conclusion, P. speciosa empty pod extract exhibited potential anti-inflammatory properties against TNF-α-induced inflammation, possibly by modulating the NF-κB p65 pathway. The effects were comparable to that of quercetin.",
keywords = "Cyclooxygenase-2, Inducible nitric oxide synthase, NF-κB p65, Reactive oxygen species, Vascular cell adhesion molecule",
author = "Mustafa, {Nor Hidayah} and Azizah Ugusman and Juriyati Jalil and Kamisah Yusof",
year = "2018",
month = "1",
day = "1",
doi = "10.7324/JAPS.2018.8123",
language = "English",
volume = "8",
pages = "152--158",
journal = "Journal of Applied Pharmaceutical Science",
issn = "2231-3354",
publisher = "MediPoeia",
number = "1",

}

TY - JOUR

T1 - Anti-inflammatory property of Parkia speciosa empty pod extract in human umbilical vein endothelial cells

AU - Mustafa, Nor Hidayah

AU - Ugusman, Azizah

AU - Jalil, Juriyati

AU - Yusof, Kamisah

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Parkia speciosa Hassk, locally known as petai papan, is a common medicinal plant found in Southeast Asia. Its empty pods were reported to contain high concentrations of polyphenols, particularly quercetin. This study aimed to evaluate the anti-inflammatory properties of P. speciosa empty pod extract in human umbilical vein endothelial cells (HUVECs). The empty pods were extracted by sequential fractionation with absolute ethanol and ethyl acetate. HUVECs were divided into four groups. HUVECs were exposed to tumor necrosis factor-α (TNF-α, 10 ng/mL) in the presence (25 μg/mL) or absence of Parkia speciosa extract. Quercetin (125 μM) served as the positive control, while another group that was not exposed to TNF-α acted as the negative control. The protein expressions of the inflammatory mediators, which were NF-κB p65, iNOS, COX-2 and VCAM-1, were significantly decreased in the P. speciosa and quercetin groups exposed to TNF-α. P. speciosa extract and quercetin also significantly reduced intracellular reactive oxygen species and nitric oxide levels as well as inducible nitric oxide synthase (iNOS) activity caused by TNF-α in HUVECs. In conclusion, P. speciosa empty pod extract exhibited potential anti-inflammatory properties against TNF-α-induced inflammation, possibly by modulating the NF-κB p65 pathway. The effects were comparable to that of quercetin.

AB - Parkia speciosa Hassk, locally known as petai papan, is a common medicinal plant found in Southeast Asia. Its empty pods were reported to contain high concentrations of polyphenols, particularly quercetin. This study aimed to evaluate the anti-inflammatory properties of P. speciosa empty pod extract in human umbilical vein endothelial cells (HUVECs). The empty pods were extracted by sequential fractionation with absolute ethanol and ethyl acetate. HUVECs were divided into four groups. HUVECs were exposed to tumor necrosis factor-α (TNF-α, 10 ng/mL) in the presence (25 μg/mL) or absence of Parkia speciosa extract. Quercetin (125 μM) served as the positive control, while another group that was not exposed to TNF-α acted as the negative control. The protein expressions of the inflammatory mediators, which were NF-κB p65, iNOS, COX-2 and VCAM-1, were significantly decreased in the P. speciosa and quercetin groups exposed to TNF-α. P. speciosa extract and quercetin also significantly reduced intracellular reactive oxygen species and nitric oxide levels as well as inducible nitric oxide synthase (iNOS) activity caused by TNF-α in HUVECs. In conclusion, P. speciosa empty pod extract exhibited potential anti-inflammatory properties against TNF-α-induced inflammation, possibly by modulating the NF-κB p65 pathway. The effects were comparable to that of quercetin.

KW - Cyclooxygenase-2

KW - Inducible nitric oxide synthase

KW - NF-κB p65

KW - Reactive oxygen species

KW - Vascular cell adhesion molecule

UR - http://www.scopus.com/inward/record.url?scp=85041359690&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85041359690&partnerID=8YFLogxK

U2 - 10.7324/JAPS.2018.8123

DO - 10.7324/JAPS.2018.8123

M3 - Article

VL - 8

SP - 152

EP - 158

JO - Journal of Applied Pharmaceutical Science

JF - Journal of Applied Pharmaceutical Science

SN - 2231-3354

IS - 1

ER -