Anti-fibrotic Actions of Roselle Extract in Rat Model of Myocardial Infarction

Shafreena Shaukat Ali, Siti Fatimah Azaharah Mohamed, Nur Hafiqah Rozalei, Yap Wei Boon, Satirah Zainalabidin

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Heart failure-associated morbidity and mortality is largely attributable to extensive and unregulated cardiac remodelling. Roselle (Hibiscus sabdariffa) calyces are enriched with natural polyphenols known for antioxidant and anti-hypertensive effects, yet its effects on early cardiac remodelling in post myocardial infarction (MI) setting are still unclear. Thus, the aim of this study was to investigate the actions of roselle extract on cardiac remodelling in rat model of MI. Male Wistar rats (200–300 g) were randomly allotted into three groups: Control, MI, and MI + Roselle. MI was induced with isoprenaline (ISO) (85 mg/kg, s.c) for two consecutive days followed by roselle treatment (100 mg/kg, orally) for 7 days. Isoprenaline administration showed changes in heart weight to body weight (HW/BW) ratio. MI was especially evident by the elevated cardiac injury marker, troponin-T, and histological observation. Upregulation of plasma levels and cardiac gene expression levels of inflammatory cytokines such as interleukin (IL)-6 and IL-10 was seen in MI rats. A relatively high percentage of fibrosis was observed in rat heart tissues with over-expression of collagen (Col)-1 and Col-3 genes following isoprenaline-induced MI. On top of that, cardiomyocyte areas were larger in heart tissues of MI rats with upregulation of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) gene expression, indicating cardiac hypertrophy. Interestingly, roselle supplementation attenuated elevation of plasma troponin-T, IL-6, IL10, and gene expression level of IL-10. Furthermore, reduction of cardiac fibrosis and hypertrophy were observed. In conclusion, roselle treatment was able to limit early cardiac remodelling in MI rat model by alleviating inflammation, fibrosis, and hypertrophy; hence, the potential application of roselle in early adjunctive treatment to prevent heart failure.

Original languageEnglish
JournalCardiovascular Toxicology
DOIs
Publication statusAccepted/In press - 1 Jan 2018

Fingerprint

Hibiscus
Rats
Myocardial Infarction
Isoproterenol
Gene expression
Interleukin-10
Troponin T
Interleukin-6
Collagen
Fibrosis
Tissue
Plasmas
Cardiomegaly
Gene Expression
Brain Natriuretic Peptide
Polyphenols
Atrial Natriuretic Factor
Up-Regulation
Heart Failure
Antihypertensive Agents

Keywords

  • Cardiac remodelling
  • Fibrosis
  • Hypertrophy
  • Inflammation
  • Isoprenaline
  • Roselle

ASJC Scopus subject areas

  • Molecular Biology
  • Toxicology
  • Cardiology and Cardiovascular Medicine

Cite this

Anti-fibrotic Actions of Roselle Extract in Rat Model of Myocardial Infarction. / Ali, Shafreena Shaukat; Mohamed, Siti Fatimah Azaharah; Rozalei, Nur Hafiqah; Boon, Yap Wei; Zainalabidin, Satirah.

In: Cardiovascular Toxicology, 01.01.2018.

Research output: Contribution to journalArticle

Ali, Shafreena Shaukat ; Mohamed, Siti Fatimah Azaharah ; Rozalei, Nur Hafiqah ; Boon, Yap Wei ; Zainalabidin, Satirah. / Anti-fibrotic Actions of Roselle Extract in Rat Model of Myocardial Infarction. In: Cardiovascular Toxicology. 2018.
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AB - Heart failure-associated morbidity and mortality is largely attributable to extensive and unregulated cardiac remodelling. Roselle (Hibiscus sabdariffa) calyces are enriched with natural polyphenols known for antioxidant and anti-hypertensive effects, yet its effects on early cardiac remodelling in post myocardial infarction (MI) setting are still unclear. Thus, the aim of this study was to investigate the actions of roselle extract on cardiac remodelling in rat model of MI. Male Wistar rats (200–300 g) were randomly allotted into three groups: Control, MI, and MI + Roselle. MI was induced with isoprenaline (ISO) (85 mg/kg, s.c) for two consecutive days followed by roselle treatment (100 mg/kg, orally) for 7 days. Isoprenaline administration showed changes in heart weight to body weight (HW/BW) ratio. MI was especially evident by the elevated cardiac injury marker, troponin-T, and histological observation. Upregulation of plasma levels and cardiac gene expression levels of inflammatory cytokines such as interleukin (IL)-6 and IL-10 was seen in MI rats. A relatively high percentage of fibrosis was observed in rat heart tissues with over-expression of collagen (Col)-1 and Col-3 genes following isoprenaline-induced MI. On top of that, cardiomyocyte areas were larger in heart tissues of MI rats with upregulation of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) gene expression, indicating cardiac hypertrophy. Interestingly, roselle supplementation attenuated elevation of plasma troponin-T, IL-6, IL10, and gene expression level of IL-10. Furthermore, reduction of cardiac fibrosis and hypertrophy were observed. In conclusion, roselle treatment was able to limit early cardiac remodelling in MI rat model by alleviating inflammation, fibrosis, and hypertrophy; hence, the potential application of roselle in early adjunctive treatment to prevent heart failure.

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