Anti-biofilm activity of the marine bacterium Pseudoalteromonas ruthenica KLPp3 against Serratia marcescens and Vibrio alginolyticus

Asmat Ahmad, Mohd Faiq Iqbal Sulieman, Gires Usup

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Aims: Pseudoalteromonas ruthenica KLPp3 is the marine Gram-negative strain isolated from the surface of mud crab at Pulau Perhentian Malaysia. In this work, the anti-biofilm activity of P. ruthenica supernatant was examined on Serratia marcescen and Vibrio alginolyticus. Methodology and results: The crude extract of P. ruthenica KLPp3 was obtained using ethyl acetate. The subminimum inhibitory concentration (MIC) of the crude extract was determined using the minimum inhibitory test. The sub- MIC crude extract was tested against two of the S. marcescen virulence factors, which are the swarming ability and production of prodigiosin. The crystal violet assay was used to test the anti-biofilm activity of the sub-MIC crude extract against S. marcescen and V. alginolyticus. The productions of prodigiosin were reduced by 72%. The swarming area was reduced by 56.06%. It inhibits 26.9% and 48.5% of biofilm production in S. marcescens and V. alginolyticus respectively. The crude extract was heat stable. Conclusion, significance and impact of study: Besides combating the S. marcescens virulence factor, P. ruthenica KLPp3 crude extract in sub-MIC reduces the formation of biofilm of S. marcescens and V. alginolyticus, which may find applications in biofilm inhibition and prevention.

Original languageEnglish
Pages (from-to)30-34
Number of pages5
JournalMalaysian Journal of Microbiology
Volume12
Issue number1
Publication statusPublished - 2016

Fingerprint

Pseudoalteromonas
Vibrio alginolyticus
Serratia marcescens
Biofilms
Complex Mixtures
Bacteria
Prodigiosin
Virulence Factors
Gentian Violet
Malaysia
Hot Temperature

Keywords

  • Anti-biofilm
  • Anti-microbial
  • Crude extract
  • Pseudoalteromonas ruthenica
  • Sub-MICs

ASJC Scopus subject areas

  • Infectious Diseases
  • Microbiology (medical)

Cite this

Anti-biofilm activity of the marine bacterium Pseudoalteromonas ruthenica KLPp3 against Serratia marcescens and Vibrio alginolyticus. / Ahmad, Asmat; Sulieman, Mohd Faiq Iqbal; Usup, Gires.

In: Malaysian Journal of Microbiology, Vol. 12, No. 1, 2016, p. 30-34.

Research output: Contribution to journalArticle

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abstract = "Aims: Pseudoalteromonas ruthenica KLPp3 is the marine Gram-negative strain isolated from the surface of mud crab at Pulau Perhentian Malaysia. In this work, the anti-biofilm activity of P. ruthenica supernatant was examined on Serratia marcescen and Vibrio alginolyticus. Methodology and results: The crude extract of P. ruthenica KLPp3 was obtained using ethyl acetate. The subminimum inhibitory concentration (MIC) of the crude extract was determined using the minimum inhibitory test. The sub- MIC crude extract was tested against two of the S. marcescen virulence factors, which are the swarming ability and production of prodigiosin. The crystal violet assay was used to test the anti-biofilm activity of the sub-MIC crude extract against S. marcescen and V. alginolyticus. The productions of prodigiosin were reduced by 72{\%}. The swarming area was reduced by 56.06{\%}. It inhibits 26.9{\%} and 48.5{\%} of biofilm production in S. marcescens and V. alginolyticus respectively. The crude extract was heat stable. Conclusion, significance and impact of study: Besides combating the S. marcescens virulence factor, P. ruthenica KLPp3 crude extract in sub-MIC reduces the formation of biofilm of S. marcescens and V. alginolyticus, which may find applications in biofilm inhibition and prevention.",
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AB - Aims: Pseudoalteromonas ruthenica KLPp3 is the marine Gram-negative strain isolated from the surface of mud crab at Pulau Perhentian Malaysia. In this work, the anti-biofilm activity of P. ruthenica supernatant was examined on Serratia marcescen and Vibrio alginolyticus. Methodology and results: The crude extract of P. ruthenica KLPp3 was obtained using ethyl acetate. The subminimum inhibitory concentration (MIC) of the crude extract was determined using the minimum inhibitory test. The sub- MIC crude extract was tested against two of the S. marcescen virulence factors, which are the swarming ability and production of prodigiosin. The crystal violet assay was used to test the anti-biofilm activity of the sub-MIC crude extract against S. marcescen and V. alginolyticus. The productions of prodigiosin were reduced by 72%. The swarming area was reduced by 56.06%. It inhibits 26.9% and 48.5% of biofilm production in S. marcescens and V. alginolyticus respectively. The crude extract was heat stable. Conclusion, significance and impact of study: Besides combating the S. marcescens virulence factor, P. ruthenica KLPp3 crude extract in sub-MIC reduces the formation of biofilm of S. marcescens and V. alginolyticus, which may find applications in biofilm inhibition and prevention.

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