Anti-amoebic properties of a Malaysian marine sponge Aaptos sp. on Acanthamoeba castellanii

M. A. Nakisah, M. Y. Ida Muryany, H. Fatimah, R. Nor Fadilah, M. R. Zalilawati, S. Khamsah, M. Habsah

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Crude methanol extracts of a marine sponge, Aaptosaaptos, collected from three different localities namely Kapas, Perhentian and Redang Islands, Terengganu, Malaysia, were tested in vitro on a pathogenic Acanthamoeba castellanii (IMR isolate) to examine their anti-amoebic potential. The examination of anti-Acanthamoebic activity of the extracts was conducted in 24 well plates for 72 h at 30 °C. All extracts possessed anti-amoebic activity with their IC50 values ranging from 0. 615 to 0. 876 mg/mL. The effect of the methanol extracts on the surface morphology of A. castellanii was analysed under scanning electron microscopy. The ability of the extracts to disrupt the amoeba cell membrane was indicated by extensive cell's blebbing, changes in the surface morphology, reduced in cell size and with cystic appearance of extract-treated Acanthamoeba. Number of acanthapodia and food cup was also reduced in this Acanthamoeba. Morphological criteria of apoptosis in Acanthamoeba following treatment with the sponge's extracts was determined by acridine orange-propidium iodide staining and observed by fluorescence microscopy. By this technique, apoptotic and necrotic cells can be visualized and quantified. The genotoxic potential of the methanol extracts was performed by the alkaline comet assay. All methanol extracts used were significantly induced DNA damage compared to untreated Acanthamoeba by having high percentage of scores 1, 2, and 3 of the DNA damage. Results from cytotoxicity and genotoxicity studies carried out in the present study suggest that all methanol extracts of A. aaptos have anti-amoebic properties against A. castellanii.

Original languageEnglish
Pages (from-to)1237-1244
Number of pages8
JournalWorld Journal of Microbiology and Biotechnology
Volume28
Issue number3
DOIs
Publication statusPublished - Mar 2012

Fingerprint

Acanthamoeba castellanii
Porifera
Acanthamoeba
Methanol
DNA Damage
Acridine Orange
Amoeba
Comet Assay
Propidium
Malaysia
Blister
Complex Mixtures
Cell Size
Fluorescence Microscopy
Islands
Electron Scanning Microscopy
Inhibitory Concentration 50
Cell Membrane
Apoptosis
Staining and Labeling

Keywords

  • Aaptos aaptos
  • Acanthamoeba castellanii
  • Apoptosis
  • Comet assay
  • IC
  • Scanning electron microscopy

ASJC Scopus subject areas

  • Biotechnology
  • Physiology
  • Applied Microbiology and Biotechnology

Cite this

Anti-amoebic properties of a Malaysian marine sponge Aaptos sp. on Acanthamoeba castellanii. / Nakisah, M. A.; Ida Muryany, M. Y.; Fatimah, H.; Nor Fadilah, R.; Zalilawati, M. R.; Khamsah, S.; Habsah, M.

In: World Journal of Microbiology and Biotechnology, Vol. 28, No. 3, 03.2012, p. 1237-1244.

Research output: Contribution to journalArticle

Nakisah, M. A. ; Ida Muryany, M. Y. ; Fatimah, H. ; Nor Fadilah, R. ; Zalilawati, M. R. ; Khamsah, S. ; Habsah, M. / Anti-amoebic properties of a Malaysian marine sponge Aaptos sp. on Acanthamoeba castellanii. In: World Journal of Microbiology and Biotechnology. 2012 ; Vol. 28, No. 3. pp. 1237-1244.
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