Anti-Acanthamoeba efficacy of the multipurpose contact lens disinfecting solutions

Mohamed Kamel Abdul Ghani, Ee Chin Liew, Anisah Nordin, Yusof Suboh, Noraina Ab Rahim, Norazah Ahmad, Wan Omar Abdullah

Research output: Contribution to journalArticle

Abstract

Introduction: Disinfections of contact lens play a crucial part in eliminating Acanthamoeba keratitis. This study was therefore performed to evaluate the effectiveness of contact lens disinfecting solutions on cysts of Acanthamoeba castellani derived clinically from keratitis case based on the exposure times suggested by manufacturers, 4 hours and 8 hours. Materials and Methods: Three brands of multipurpose contact lens disinfecting solutions currently available in the market consisting of Freshlook All in OneTM, Solo Care® Plusc and Renu Multi-Plus® were tested. Suspension of cysts was exposed to the disinfecting solutions at intervals varying from 5 minutes to 8 hours and later cultured onto non-nutrient agar lawned with Escherichia coli for the growth of Acanthamoeba trophozoites. Results: All three multipurpose contact lens disinfecting solutions (Freshlook All in OneTM, Solo Care® Plusc and Renu Multi-Plus®) showed no effect on Acanthamoeba castellani cysts in time suggested by the manufacturers, 4 hours, and 8 hours. Conclusion: This study has proven that though contact lens disinfecting solutions have antimicrobial properties, not all are effective against Acanthamoeba cysts. The search for a solution which is effective against Acanthamoeba cysts would be desirable for contact lens wearers.

Original languageEnglish
Pages (from-to)512-514
Number of pages3
JournalInternational Medical Journal
Volume23
Issue number5
Publication statusPublished - 2016

Fingerprint

Contact Lens Solutions
Acanthamoeba
Cysts
Contact Lenses
Acanthamoeba Keratitis
Trophozoites
Keratitis
Disinfection
Agar
Suspensions
Escherichia coli
Growth

Keywords

  • Acanthamoeba
  • Malaysia
  • Multipurpose contact lens disinfecting solutions

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Abdul Ghani, M. K., Liew, E. C., Nordin, A., Suboh, Y., Rahim, N. A., Ahmad, N., & Abdullah, W. O. (2016). Anti-Acanthamoeba efficacy of the multipurpose contact lens disinfecting solutions. International Medical Journal, 23(5), 512-514.

Anti-Acanthamoeba efficacy of the multipurpose contact lens disinfecting solutions. / Abdul Ghani, Mohamed Kamel; Liew, Ee Chin; Nordin, Anisah; Suboh, Yusof; Rahim, Noraina Ab; Ahmad, Norazah; Abdullah, Wan Omar.

In: International Medical Journal, Vol. 23, No. 5, 2016, p. 512-514.

Research output: Contribution to journalArticle

Abdul Ghani, MK, Liew, EC, Nordin, A, Suboh, Y, Rahim, NA, Ahmad, N & Abdullah, WO 2016, 'Anti-Acanthamoeba efficacy of the multipurpose contact lens disinfecting solutions', International Medical Journal, vol. 23, no. 5, pp. 512-514.
Abdul Ghani, Mohamed Kamel ; Liew, Ee Chin ; Nordin, Anisah ; Suboh, Yusof ; Rahim, Noraina Ab ; Ahmad, Norazah ; Abdullah, Wan Omar. / Anti-Acanthamoeba efficacy of the multipurpose contact lens disinfecting solutions. In: International Medical Journal. 2016 ; Vol. 23, No. 5. pp. 512-514.
@article{44a5f46dbca1436aa71aa35e0f61a3f1,
title = "Anti-Acanthamoeba efficacy of the multipurpose contact lens disinfecting solutions",
abstract = "Introduction: Disinfections of contact lens play a crucial part in eliminating Acanthamoeba keratitis. This study was therefore performed to evaluate the effectiveness of contact lens disinfecting solutions on cysts of Acanthamoeba castellani derived clinically from keratitis case based on the exposure times suggested by manufacturers, 4 hours and 8 hours. Materials and Methods: Three brands of multipurpose contact lens disinfecting solutions currently available in the market consisting of Freshlook All in OneTM, Solo Care{\circledR} Plusc and Renu Multi-Plus{\circledR} were tested. Suspension of cysts was exposed to the disinfecting solutions at intervals varying from 5 minutes to 8 hours and later cultured onto non-nutrient agar lawned with Escherichia coli for the growth of Acanthamoeba trophozoites. Results: All three multipurpose contact lens disinfecting solutions (Freshlook All in OneTM, Solo Care{\circledR} Plusc and Renu Multi-Plus{\circledR}) showed no effect on Acanthamoeba castellani cysts in time suggested by the manufacturers, 4 hours, and 8 hours. Conclusion: This study has proven that though contact lens disinfecting solutions have antimicrobial properties, not all are effective against Acanthamoeba cysts. The search for a solution which is effective against Acanthamoeba cysts would be desirable for contact lens wearers.",
keywords = "Acanthamoeba, Malaysia, Multipurpose contact lens disinfecting solutions",
author = "{Abdul Ghani}, {Mohamed Kamel} and Liew, {Ee Chin} and Anisah Nordin and Yusof Suboh and Rahim, {Noraina Ab} and Norazah Ahmad and Abdullah, {Wan Omar}",
year = "2016",
language = "English",
volume = "23",
pages = "512--514",
journal = "International Medical Journal",
issn = "1341-2051",
publisher = "Japan International Cultural Exchange Foundation",
number = "5",

}

TY - JOUR

T1 - Anti-Acanthamoeba efficacy of the multipurpose contact lens disinfecting solutions

AU - Abdul Ghani, Mohamed Kamel

AU - Liew, Ee Chin

AU - Nordin, Anisah

AU - Suboh, Yusof

AU - Rahim, Noraina Ab

AU - Ahmad, Norazah

AU - Abdullah, Wan Omar

PY - 2016

Y1 - 2016

N2 - Introduction: Disinfections of contact lens play a crucial part in eliminating Acanthamoeba keratitis. This study was therefore performed to evaluate the effectiveness of contact lens disinfecting solutions on cysts of Acanthamoeba castellani derived clinically from keratitis case based on the exposure times suggested by manufacturers, 4 hours and 8 hours. Materials and Methods: Three brands of multipurpose contact lens disinfecting solutions currently available in the market consisting of Freshlook All in OneTM, Solo Care® Plusc and Renu Multi-Plus® were tested. Suspension of cysts was exposed to the disinfecting solutions at intervals varying from 5 minutes to 8 hours and later cultured onto non-nutrient agar lawned with Escherichia coli for the growth of Acanthamoeba trophozoites. Results: All three multipurpose contact lens disinfecting solutions (Freshlook All in OneTM, Solo Care® Plusc and Renu Multi-Plus®) showed no effect on Acanthamoeba castellani cysts in time suggested by the manufacturers, 4 hours, and 8 hours. Conclusion: This study has proven that though contact lens disinfecting solutions have antimicrobial properties, not all are effective against Acanthamoeba cysts. The search for a solution which is effective against Acanthamoeba cysts would be desirable for contact lens wearers.

AB - Introduction: Disinfections of contact lens play a crucial part in eliminating Acanthamoeba keratitis. This study was therefore performed to evaluate the effectiveness of contact lens disinfecting solutions on cysts of Acanthamoeba castellani derived clinically from keratitis case based on the exposure times suggested by manufacturers, 4 hours and 8 hours. Materials and Methods: Three brands of multipurpose contact lens disinfecting solutions currently available in the market consisting of Freshlook All in OneTM, Solo Care® Plusc and Renu Multi-Plus® were tested. Suspension of cysts was exposed to the disinfecting solutions at intervals varying from 5 minutes to 8 hours and later cultured onto non-nutrient agar lawned with Escherichia coli for the growth of Acanthamoeba trophozoites. Results: All three multipurpose contact lens disinfecting solutions (Freshlook All in OneTM, Solo Care® Plusc and Renu Multi-Plus®) showed no effect on Acanthamoeba castellani cysts in time suggested by the manufacturers, 4 hours, and 8 hours. Conclusion: This study has proven that though contact lens disinfecting solutions have antimicrobial properties, not all are effective against Acanthamoeba cysts. The search for a solution which is effective against Acanthamoeba cysts would be desirable for contact lens wearers.

KW - Acanthamoeba

KW - Malaysia

KW - Multipurpose contact lens disinfecting solutions

UR - http://www.scopus.com/inward/record.url?scp=85025674835&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85025674835&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:85025674835

VL - 23

SP - 512

EP - 514

JO - International Medical Journal

JF - International Medical Journal

SN - 1341-2051

IS - 5

ER -