Aktiviti Anti-Acanthamoeba oleh disinfektan kanta sentuh pelbagai guna terhadap sista acanthamoeba pencilan klinikal dan persekitaran

Translated title of the contribution: Anti-acanthamoeba activity of multi-purpose contact lens disinfecting solutions against acanthamoeba cysts of clinical and environmental isolates

Mohamed Kamel Abdul Ghani, Syamimi Omar, Anisah Nordin, Yusof Suboh, Noraina Ab Rahim, Wan Omar Abdullah, Norazah Ahmad

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Effective disinfection of contact lenses is of paramount importance in the prevention of Acanthamoeba (AK). Therefore, this study was undertaken to evaluate the effectiveness of the multi-purpose contact lens disinfecting solutions against Acanthamoeba cysts of four isolates comprising two clinical isolates; HS 5, HTH 73, and two environmental isolates; TTA 1 and TKA 14. Five multi-purpose contact lens disinfecting solutions; Complete®, Renu® fresh™, RevitaLens OcuTec®, Opti-Free® Express® and Solo Care Aqua® were tested on cyst of the four Acanthamoeba isolates. The soaking times were based on manufactures recommendations (4 and 6 h), 8 h and 24 h. Each mixture of the cysts and disinfecting solution was transferred onto non-nutrient agar seeded with Escherichia coli. The agar plates were examined under inverted microscope daily until day 14 to detect the presence of Acanthamoeba trophozoites. All 5 multi-purpose contact lens disinfecting solutions tested were ineffective at killing all Acanthamoeba cyst isolates tested. This indicates that most multi-purpose contact lens disinfecting solutions do not have anti-Acanthamoeba activity necessary to prevent keratitis amongst the contact lens wearers.

Original languageMalay
Pages (from-to)609-613
Number of pages5
JournalSains Malaysiana
Volume45
Issue number4
Publication statusPublished - 1 Apr 2016

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Acanthamoeba
Lens
agar
keratitis
trophozoites
disinfection
soaking
microscopes
manufacturing
Escherichia coli

ASJC Scopus subject areas

  • General

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Aktiviti Anti-Acanthamoeba oleh disinfektan kanta sentuh pelbagai guna terhadap sista acanthamoeba pencilan klinikal dan persekitaran. / Abdul Ghani, Mohamed Kamel; Omar, Syamimi; Nordin, Anisah; Suboh, Yusof; Rahim, Noraina Ab; Abdullah, Wan Omar; Ahmad, Norazah.

In: Sains Malaysiana, Vol. 45, No. 4, 01.04.2016, p. 609-613.

Research output: Contribution to journalArticle

Abdul Ghani, Mohamed Kamel ; Omar, Syamimi ; Nordin, Anisah ; Suboh, Yusof ; Rahim, Noraina Ab ; Abdullah, Wan Omar ; Ahmad, Norazah. / Aktiviti Anti-Acanthamoeba oleh disinfektan kanta sentuh pelbagai guna terhadap sista acanthamoeba pencilan klinikal dan persekitaran. In: Sains Malaysiana. 2016 ; Vol. 45, No. 4. pp. 609-613.
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